Cat. No.: HY-B0647

Butylphthalide

(Synonyms: 3-n-Butylphthalide; 3-Butylphthalide)

11 Cited Publications

Customer Review

Based on 11 publication(s) in Google Scholar

Butylphthalide (3-n-Butylphthalide) is an active molecule against cerebral ischemia. It was originally isolated from celery species and has been shown to be effective in stroke animal models.

For research use only. We do not sell to patients.

Purity: 99.98%
CAS No.: 6066-49-5
Formula: C₁₂H₁₄O₂
Molecular Weight:190.24

Storage:

Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

Butylphthalide Chemical Structure

CAS No.: 6066-49-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vivo
- Clinical Trial
Chemical Information
Publications (11)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

Butylphthalide (3-n-Butylphthalide) is an active molecule against cerebral ischemia. It was originally isolated from celery species and has been shown to be effective in stroke animal models.

All p38 MAPK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Platelet	IC₅₀	>1 mM Compound: NBP	Inhibition of adenosine diphosphate-induced platelet aggregation in rabbit platelet rich plasma by Born's turbidimetric method Inhibition of adenosine diphosphate-induced platelet aggregation in rabbit platelet rich plasma by Born's turbidimetric method	[PMID: 22827516]
Platelet	IC₅₀	0.48 mM Compound: NBP	Inhibition of arachidonic acid-induced platelet aggregation in rabbit platelet rich plasma by Born's turbidimetric method Inhibition of arachidonic acid-induced platelet aggregation in rabbit platelet rich plasma by Born's turbidimetric method	[PMID: 22827516]
Platelet	IC₅₀	0.74 mM Compound: NBP	Antiplatelet activity in rabbit platelet-rich plasma assessed as inhibition of ADP-induced platelet aggregation preincubated for 5 mins followed by addition of ADP measured within 5 mins by Borns turbidimetric method Antiplatelet activity in rabbit platelet-rich plasma assessed as inhibition of ADP-induced platelet aggregation preincubated for 5 mins followed by addition of ADP measured within 5 mins by Borns turbidimetric method	[PMID: 27031213]

In Vivo

Butylphthalide (160mg/kg, i.g) improves learning and memory deficits in SAMP8 mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Old male SAMP8 mice and SAMR1 mice^[3]
Dosage:

40 mg/kg, 80 mg/kg, 160 mg/kg
Administration:

Orally daily, 60 days
Result:

Shorted escape latency time on day 7.
Improved SAMP8 mice learning deficits.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 6066-49-5
Appearance Liquid (Density: 1.0527 g/cm³)
Molecular Weight 190.24
Formula C₁₂H₁₄O₂
Color Colorless to light yellow
SMILES

O=C1OC(CCCC)C2=C1C=CC=C2
Synonyms

3-n-Butylphthalide; 3-Butylphthalide
Structure Classification
- Others
Initial Source
- Plants
- Umbelliferae
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (11)

All Histological Imaging/StainingIFCell Migration/Invasion AssayApoptosis AnalysisWB

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Journal Impact Factor

J Adv Res

Butylphthalide enhances neurorestoration following ischemic stroke by restructuring microvasculature through vimentin modulation. [Abstract]

2025 Dec 7:S2090-1232(25)00964-6. PMID: 41365434
J Adv Res

Targeting MAD2B as a strategy for ischemic stroke therapy. [Abstract]

2024 Jul 5:S2090-1232(24)00269-8. PMID: 38972542
Phytomedicine

Huang-Qi-Long-Dan Granule alleviates ischemic stroke injury by regulating the crosstalk between Nrf2 and NF-κB signaling. [Abstract]

2026 Jun:155:158069. PMID: 41861685
Front Pharmacol

Unveiling the potential of Butylphthalide: inhibiting osteoclastogenesis and preventing bone loss. [Abstract]

2024 Feb 23:15:1347241. PMID: 38464734
World J Diabetes

Dl-3-n-butylphthalide ameliorates diabetic foot ulcer by inhibiting apoptosis and promoting angiogenesis. [Abstract]

2025 Apr 15;16(4):101916. PMID: 40236845

Butylphthalide purchased from MedChemExpress. Usage Cited in: World J Diabetes. 2025 Apr 15;16(4):101916. [Abstract]

The HE and Masson’s trichrome staining results showed that the Butylphthalide (NBP) (10 μM) group significantly increased neovascularization and fibroblast proliferation relative to the model group.

Butylphthalide purchased from MedChemExpress. Usage Cited in: World J Diabetes. 2025 Apr 15;16(4):101916. [Abstract]

Immunofluorescence results demonstrated higher expressions of Ki67 and CD31 in the Butylphthalide (NBP) (10 μM) group than in the model group.

Butylphthalide purchased from MedChemExpress. Usage Cited in: World J Diabetes. 2025 Apr 15;16(4):101916. [Abstract]

Butylphthalide (NBP) (15 μM) treatment significantly enhanced the proliferation of HUVEC and HDF cells under HG conditions.

Butylphthalide purchased from MedChemExpress. Usage Cited in: World J Diabetes. 2025 Apr 15;16(4):101916. [Abstract]

The flow cytometry detection results showed that, compared to the HG group, the apoptosis rate in the Butylphthalide (NBP) (15 μM) + HG group was markedly reduced in HUVEC, HaCaT, and HDF cells.

Butylphthalide purchased from MedChemExpress. Usage Cited in: World J Diabetes. 2025 Apr 15;16(4):101916. [Abstract]

Butylphthalide (NBP) (15 μM) activated the AGE-RAGE signaling pathway and modulated the biological functions of HUVEC, HaCaT, and HDF cells, to treat DFU by Western blot.
Front Aging Neurosci

Dl-3- n-Butylphthalide Exerts Dopaminergic Neuroprotection Through Inhibition of Neuroinflammation. [Abstract]

2019 Feb 28:11:44. PMID: 30873019

Butylphthalide purchased from MedChemExpress. Usage Cited in: Front Aging Neurosci. 2019 Feb 28:11:44. [Abstract]

The protein level of TNF-α, IL-1β, iNOS and COX2 is analyzed by Western Blot with or without the treatment of Butylphthalide (NBP) and LPS.
Mol Neurobiol

3-n-Butylphthalide Protects SH-SY5Y Cells from Ferroptosis by Inhibiting ACSL4-Mediated Lipid Peroxidation. [Abstract]

2026 Jan 16;63(1):369. PMID: 41545774
Food Sci Nutr

Traditional Chinese Herbal Formula XuMingZhuSan Alleviates Neuronal Ferroptosis in Rats After Stroke by Activating the NRF2/GPX4/SLC7A11 Pathway. [Abstract]

2026 Mar 10;14(3):e71495. PMID: 41822239
Chem Biol Drug Des

Dl-3-n-Butylphthalide Inhibits Autophagy to Alleviate the Neuronal Apoptosis After Spinal Cord Injury by Elevating YAP via Inactivating the Hippo Signaling Pathway. [Abstract]

2025 Oct;106(4):e70180. PMID: 41104600
Front Biosci (Landmark Ed)

Ginkgolide B Promotes Angiogenesis After Oxygen-Glucose Deprivation by Regulating AKT1 in bEnd.3 Cells. [Abstract]

2025 Dec 15;30(12):46724. PMID: 41504064
Oxid Med Cell Longev

DL-3-N-Butylphthalide Promotes Cartilage Extracellular Matrix Synthesis and Inhibits Osteoarthritis Development by Regulating FoxO3a. [Abstract]

2022 Jul 20:2022:9468040. PMID: 35910845

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (1314.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.2565 mL

26.2823 mL

52.5646 mL

5 mM

1.0513 mL

5.2565 mL

10.5129 mL

10 mM

0.5256 mL

2.6282 mL

5.2565 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (10.93 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (10.93 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (10.93 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline

Solubility: 13.33 mg/mL (70.07 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Cui LY, et al. Ninety-day administration of dl-3-n-butylphthalide for acute ischemic stroke: a randomized, double-blind trial. Chin Med J (Engl). 2013;126(18):3405-10. [Content Brief]

[2]. Zhao W, et al. 3-N-butylphthalide improves neuronal morphology after chronic cerebral ischemia. Neural Regen Res. 2014 Apr 1;9(7):719-26. [Content Brief]

[3]. Wang X, et al. Design, synthesis and biological evaluation of hydrogen sulfide releasing derivatives of 3-n-butylphthalide as potential antiplatelet and antithrombotic agents. Org Biomol Chem. 2014 Aug 21;12(31):5995-6004. [Content Brief]

[4]. Wang F, et al. Improvement of cognitive deficits in SAMP8 mice by 3-n-butylphthalide. Neurol Res. 2014 Mar;36(3):224-33. [Content Brief]

[5]. Chen XQ, Qiu K, Liu H, He Q, Bai JH, Lu W. Application and prospects of butylphthalide for the treatment of neurologic diseases. Chin Med J (Engl). 2019;132(12):1467-1477. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.2565 mL	26.2823 mL	52.5646 mL	131.4116 mL
	5 mM	1.0513 mL	5.2565 mL	10.5129 mL	26.2823 mL
	10 mM	0.5256 mL	2.6282 mL	5.2565 mL	13.1412 mL
	15 mM	0.3504 mL	1.7522 mL	3.5043 mL	8.7608 mL
	20 mM	0.2628 mL	1.3141 mL	2.6282 mL	6.5706 mL
	25 mM	0.2103 mL	1.0513 mL	2.1026 mL	5.2565 mL
	30 mM	0.1752 mL	0.8761 mL	1.7522 mL	4.3804 mL
	40 mM	0.1314 mL	0.6571 mL	1.3141 mL	3.2853 mL
	50 mM	0.1051 mL	0.5256 mL	1.0513 mL	2.6282 mL
	60 mM	0.0876 mL	0.4380 mL	0.8761 mL	2.1902 mL
	80 mM	0.0657 mL	0.3285 mL	0.6571 mL	1.6426 mL
	100 mM	0.0526 mL	0.2628 mL	0.5256 mL	1.3141 mL

Keywords

Butylphthalide

11 Cited Publications

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Butylphthalide

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Publications Citing Use of MedChemExpress (MCE) Butylphthalide

All p38 MAPK Isoforms

Biological Activity

Chemical Information

Publications (11)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Butylphthalide Related Classifications

Cat. No.: HY-B0647