1. Immunology/Inflammation
  2. Toll-like Receptor (TLR)

CU-CPT-8m (Synonyms: TLR8-specific antagonist)

Cat. No.: HY-112050 Purity: 99.93%
Handling Instructions

CU-CPT-8m is a specific TLR8 antagonist, with an IC50 of 67 nM.

For research use only. We do not sell to patients.

CU-CPT-8m Chemical Structure

CU-CPT-8m Chemical Structure

CAS No. : 125079-83-6

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 132 In-stock
Estimated Time of Arrival: December 31
5 mg USD 120 In-stock
Estimated Time of Arrival: December 31
10 mg USD 190 In-stock
Estimated Time of Arrival: December 31
50 mg USD 690 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1100 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

  • Biological Activity

  • Technical Information

  • Purity & Documentation

  • References

Description

CU-CPT-8m is a specific TLR8 antagonist, with an IC50 of 67 nM.

IC50 & Target

TLR8

67 nM (IC50)

In Vitro

CU-CPT-8m is a specific TLR8 antagonist, with an IC50 of 67±10 nM and negligible cytotoxicity. The Kd value of CU-CPT-8m is determined to be 220 nM. CU-CPT-8m only reduces the proinflammatory response in the TLR8-overexpressing cells strongly supports that CU-CPT-8m directly recognizes TLR8 in cells. It is particularly notable that TLR7 signaling is not affected at concentrations up to 75 μM. TLR7 and TLR8 are closely related and share many common ligands. Treatment of 1 μM CU-CPT-8m completely abolishes the elevation of TNF-α and IL-8 mRNA levels induced by R848. CU-CPT-8m inhibits R848-induced TNF-α production in the differentiated THP-1 monocytes cells in a dose-dependent manner with an IC50 of 90±10 nM, which is in good agreement with its IC50 value determined in HEK-Blue TLR8 cells[1].

Solvent & Solubility
In Vitro: 

DMSO : 30 mg/mL (118.92 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9640 mL 19.8200 mL 39.6401 mL
5 mM 0.7928 mL 3.9640 mL 7.9280 mL
10 mM 0.3964 mL 1.9820 mL 3.9640 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.5 mg/mL (9.91 mM); Suspended solution; Need ultrasonic and warming

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (9.91 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 1.25 mg/mL (4.96 mM); Clear solution; Need ultrasonic and warming

References
Molecular Weight

252.27

Formula

C₁₄H₁₂N₄O

CAS No.

125079-83-6

SMILES

O=C(C1=C2N=CC=C(C3=CC=CC(C)=C3)N2N=C1)N

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
CU-CPT-8m
Cat. No.:
HY-112050
Quantity:

CU-CPT-8m

Cat. No.: HY-112050