1. Epigenetics
  2. DNA Methyltransferase
  3. DC-05

DC-05 

Cat. No.: HY-12746 Purity: 99.15%
Handling Instructions

DC-05 is a DNA methyltransferase 1 (DNMT1) inhibitor, with an IC50 and a Kd of 10.3 μM and 1.09 μM, respectively.

For research use only. We do not sell to patients.

DC-05 Chemical Structure

DC-05 Chemical Structure

CAS No. : 890643-16-0

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 462 In-stock
Estimated Time of Arrival: December 31
5 mg USD 420 In-stock
Estimated Time of Arrival: December 31
10 mg USD 600 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1800 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Description

DC-05 is a DNA methyltransferase 1 (DNMT1) inhibitor, with an IC50 and a Kd of 10.3 μM and 1.09 μM, respectively.

IC50 & Target[1]

DNMT1

1.09 μM (Kd)

DNMT1

10.3 μM (IC50)

PRMT1

37.1 μM (IC50)

In Vitro

DC-05 is a DNA methyltransferase 1 (DNMT1) inhibitor, with an IC50 and a Kd of 10.3 μM and 1.09 μM, respectively. DC-05 shows less potent activities against DNMT3A, DNMT3B, G9a, SUV39H1, MLL1, SET7/9, and PRMT1 (IC50: >200, >200, >150, >150, >150, >150, 37.1 μM). DC-05 (1.25, 2.5, 5, and 10 μM) potently inhibits the proliferation of HCT116 (human colon cancer) and Capan-1 (human pancreatic adenocarcinoma cells) after treatment for 24, 48, and 72 h[1].

Molecular Weight

383.49

Formula

C₂₅H₂₅N₃O

CAS No.

890643-16-0

SMILES

OC(CNCCC1=CNC2=C1C=CC=C2)CN(C3=CC=CC=C34)C5=C4C=CC=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (260.76 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6076 mL 13.0381 mL 26.0763 mL
5 mM 0.5215 mL 2.6076 mL 5.2153 mL
10 mM 0.2608 mL 1.3038 mL 2.6076 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.52 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Kinase Assay
[1]

To measure the effects of DC-05 on mouse DNMT1 activity, 200 nM purified DNMT1 is incubated with 200 μM of DC-05 and S-adenosylmethionine (AdoMet) in the DNMT assay buffer in the assay plate at 37°C for 2 h. Next, every sample is incubated with the capture and detection antibody, followed by incubation with developer solution for 10 min at room temperature. The absorbance is measured at 450 nm using a microplate reader. S-Adenosylhomocysteine (AdoHcy) is used as a positive control[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

DC-05DC05DC 05DNA MethyltransferaseDNMTsDNA MTasesInhibitorinhibitorinhibit

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