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  3. Diphenhydramine hydrochloride

Diphenhydramine hydrochloride is a first-generation histamine H1-receptor antagonist with anti-cholinergic effect. Diphenhydramine hydrochloride can across the ovine blood-brain barrier (BBB).

For research use only. We do not sell to patients.

Diphenhydramine hydrochloride Chemical Structure

Diphenhydramine hydrochloride Chemical Structure

CAS No. : 147-24-0

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10 mM * 1 mL in DMSO
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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Diphenhydramine hydrochloride:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

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Description

Diphenhydramine hydrochloride is a first-generation histamine H1-receptor antagonist with anti-cholinergic effect. Diphenhydramine hydrochloride can across the ovine blood-brain barrier (BBB)[1][2].

IC50 & Target

H1 Receptor

 

In Vitro

Diphenhydramine hydrochloride inhibits Cisplatin (HY-17394)-induced cell death in kidney proximal tubular cells (HK-2 and LLC-PK1 cells)[3].
Diphenhydramine (0.2-1 mM, 24 h) hydrochloride inhibits cell viability in CCRF-CEM and Jurkat cells[4].
Diphenhydramine (0.5 and 1 mM, 24 h) hydrochloride induces apoptosis in CCRF-CEM and Jurkat cells[4].
Diphenhydramine (0-10 μg/mL, 24 or 48 h) hydrochloride increases the expression of Bad and Bax, and decreases BCL-2 level in PANC-1 cells[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[5]

Cell Line: PANC-1 cells
Concentration: 0-10 μg/mL
Incubation Time: 24 or 48 h
Result: Increased the expression of Bad and Bax, and decreased Bcl2 level.
Decreased the expression of p-AKT (Thr308), p-AKT (Ser 473), p-mTOR (Ser 2448), p-FoxO1 (Ser 256), p-MDM2 (Ser 166), p-NF-ĸB p65 (Ser 536), and p-GSK-3 (Ser 9).
In Vivo

Diphenhydramine (20 mg/kg) hydrochloride inhibits Cisplatin (HY-17394)-induced kidney toxicity in mice[3].
Diphenhydramine (3-30 mg/kg, i.m., 5 minutes prior to a s.c. of 25 mg/kg Dichlorvos) hydrochloride reduces mortality in rats with acute, severe dichlorvos exposure[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial
Molecular Weight

291.82

Formula

C17H22ClNO

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CN(C)CCOC(C1=CC=CC=C1)C2=CC=CC=C2.Cl

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (342.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 100 mg/mL (342.68 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4268 mL 17.1338 mL 34.2677 mL
5 mM 0.6854 mL 3.4268 mL 6.8535 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.57 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.57 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 130 mg/mL (445.48 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 3.4268 mL 17.1338 mL 34.2677 mL 85.6692 mL
5 mM 0.6854 mL 3.4268 mL 6.8535 mL 17.1338 mL
10 mM 0.3427 mL 1.7134 mL 3.4268 mL 8.5669 mL
15 mM 0.2285 mL 1.1423 mL 2.2845 mL 5.7113 mL
20 mM 0.1713 mL 0.8567 mL 1.7134 mL 4.2835 mL
25 mM 0.1371 mL 0.6854 mL 1.3707 mL 3.4268 mL
30 mM 0.1142 mL 0.5711 mL 1.1423 mL 2.8556 mL
40 mM 0.0857 mL 0.4283 mL 0.8567 mL 2.1417 mL
50 mM 0.0685 mL 0.3427 mL 0.6854 mL 1.7134 mL
60 mM 0.0571 mL 0.2856 mL 0.5711 mL 1.4278 mL
80 mM 0.0428 mL 0.2142 mL 0.4283 mL 1.0709 mL
100 mM 0.0343 mL 0.1713 mL 0.3427 mL 0.8567 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Diphenhydramine hydrochloride
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