Gly-Arg-Gly-Asp-Ser
Based on 1 Customer Validation
Gly-Arg-Gly-Asp-Ser (GRGDS) is a pentapeptide that forms the cell-binding domain of a glycoprotein, osteopontin. Gly-Arg-Gly-Asp-Ser binds to integrin receptors αvβ3 and αvβ5 with estimated IC50 of ～5 and ～6.5 μM
For research use only. We do not sell to patients.
- Purity: 99.83%
- CAS No.: 96426-21-0
- Formula: C17H30N8O9
- Molecular Weight:490.47
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
IC50: 5 μM (αvβ3), 6.5 μM (αvβ5)[2]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CCRF-CEM | IC50 |
>1000 μM
Compound: 4
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Antagonist activity at integrin alpha4beta1 receptor in human CCRF-CEM cells assessed as inhibition of cell adhesion to fibronectin after 1 hr by calcein AM staining-based fluorescence assay
Antagonist activity at integrin alpha4beta1 receptor in human CCRF-CEM cells assessed as inhibition of cell adhesion to fibronectin after 1 hr by calcein AM staining-based fluorescence assay
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10.1039/C1MD00229E |
| CHO | IC50 |
100 μM
Compound: GRGDS
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Inhibition of integrin alphaVbeta1 (unknown origin)-mediated CHO cell adhesion to fibronectin preincubated for 15 to 30 mins followed by 60 min incubation for adhesion by crystal violet staining based cell adhesion assay
Inhibition of integrin alphaVbeta1 (unknown origin)-mediated CHO cell adhesion to fibronectin preincubated for 15 to 30 mins followed by 60 min incubation for adhesion by crystal violet staining based cell adhesion assay
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[PMID: 27774126] |
Gly-Arg-Gly-Asp-Ser binds to integrin receptors αvβ3, with IC50 of 5 μM[1]. Gly-Arg-Gly-Asp-Ser-immobilized TiO2 nanotubes enhance osteoblast-like cell (MG-63) adhesion, and significantly increased cell spreading and proliferation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 96426-21-0
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Appearance Solid
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Molecular Weight 490.47
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Formula C17H30N8O9
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Color White to off-white
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Synonyms
GRGDS
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Sequence
Gly-Arg-Gly-Asp-Ser
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Sequence Shortening
GRGDS
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 50 mg/mL (101.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 25 mg/mL (50.97 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (203.89 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Bernhagen D, et al. High-Affinity RGD-Knottin Peptide as a New Tool for Rapid Evaluation of the Binding Strength of Unlabeled RGD-Peptides to αvβ3, αvβ5, and α5β1 Integrin Receptors. Anal Chem. 2017 Jun 6;89(11):5991-5997. [Content Brief]
[2]. Kim GH, et al. Evaluation of Osteoblast-Like Cell Viability and Differentiation on the Gly-Arg-Gly-Asp-Ser Peptide Immobilized Titanium Dioxide Nanotube via Chemical Grafting. J Nanosci Nanotechnol. 2016 Feb;16(2):1396-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.0389 mL | 10.1943 mL | 20.3886 mL | 50.9715 mL |
| 5 mM | 0.4078 mL | 2.0389 mL | 4.0777 mL | 10.1943 mL | |
| 10 mM | 0.2039 mL | 1.0194 mL | 2.0389 mL | 5.0972 mL | |
| 15 mM | 0.1359 mL | 0.6796 mL | 1.3592 mL | 3.3981 mL | |
| 20 mM | 0.1019 mL | 0.5097 mL | 1.0194 mL | 2.5486 mL | |
| 25 mM | 0.0816 mL | 0.4078 mL | 0.8155 mL | 2.0389 mL | |
| 30 mM | 0.0680 mL | 0.3398 mL | 0.6796 mL | 1.6991 mL | |
| 40 mM | 0.0510 mL | 0.2549 mL | 0.5097 mL | 1.2743 mL | |
| 50 mM | 0.0408 mL | 0.2039 mL | 0.4078 mL | 1.0194 mL | |
| DMSO | 60 mM | 0.0340 mL | 0.1699 mL | 0.3398 mL | 0.8495 mL |
| 80 mM | 0.0255 mL | 0.1274 mL | 0.2549 mL | 0.6371 mL | |
| 100 mM | 0.0204 mL | 0.1019 mL | 0.2039 mL | 0.5097 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Gly-Arg-Gly-Asp-Ser Related Classifications
HY-P0295 Related Classifications
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