1. Stem Cell/Wnt
  2. Wnt
    β-catenin
  3. KY1220

KY1220 

Cat. No.: HY-102028 Purity: >98.0%
Handling Instructions

KY1220 is a compound that destabilizes both β-catenin and Ras, via targeting the Wnt/β-catenin pathway; with an IC50 of 2.1 μM in HEK293 reporter cells.

For research use only. We do not sell to patients.

KY1220 Chemical Structure

KY1220 Chemical Structure

CAS No. : 292168-79-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 138 In-stock
Estimated Time of Arrival: December 31
2 mg USD 80 In-stock
Estimated Time of Arrival: December 31
5 mg USD 150 In-stock
Estimated Time of Arrival: December 31
10 mg USD 250 In-stock
Estimated Time of Arrival: December 31
25 mg USD 550 In-stock
Estimated Time of Arrival: December 31
50 mg USD 940 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1600 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products

    KY1220 purchased from MCE. Usage Cited in: Cancer Lett. 2018 Sep 28;432:121-131.

    The capability of ASPP2 in regulating epithelial–mesenchymal transition (EMT) is completely abolished by specific Wnt/β-catenin inhibitor KY1220.

    KY1220 purchased from MCE. Usage Cited in: Life Sci. 2018 Nov 1;212:129-137.

    KY1220 reverses the increasing expression of β-Catenin after upregulating Cry1 (P<0.05).
    • Biological Activity

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    Description

    KY1220 is a compound that destabilizes both β-catenin and Ras, via targeting the Wnt/β-catenin pathway; with an IC50 of 2.1 μM in HEK293 reporter cells.

    IC50 & Target

    IC50: 2.1 μM (HEK293 reporter cells)[1]

    In Vitro

    KY1220 shows an IC50 of 2.1 μM in HEK293 reporter cells. KY1220 dose dependently decreases Wnt3a-CM-induced TOPflash reporter activation and mRNA expression of Wnt target genes CCND1 and MYC in HEK293 cells. In HEK293 cells, both β-catenin and panRas protein levels are similarly reduced in a dose-dependent manner after treatment with KY1220, whereas the mRNA levels of CTNNB1 (which encodes β-catenin), NRAS, KRAS and HRAS remain unchanged. K-Ras, which has a critical role in progression of CRCs, is also destabilized by KY1220 via polyubiquitin-dependent proteasomal degradation. KY1220 accelerates the degradation rates of both β-catenin and Ras in SW480 cell lines. Ras destabilization by KY1220 consequently inhibits the activities of both ERK and Akt, which are downstream effectors of Ras in SW480 cells harboring a KRAS mutation. The proliferation and transformation of the HCT15, SW480, D-WT and D-MT CRC cells are efficiently inhibited after treatment with KY1220[1].

    Molecular Weight

    314.32

    Formula

    C₁₄H₁₀N₄O₃S

    CAS No.

    292168-79-7

    SMILES

    O=C(/C(N1)=C/C2=CC=CN2C3=CC=C([N+]([O-])=O)C=C3)NC1=S

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (318.15 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1815 mL 15.9074 mL 31.8147 mL
    5 mM 0.6363 mL 3.1815 mL 6.3629 mL
    10 mM 0.3181 mL 1.5907 mL 3.1815 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Cell Assay
    [1]

    HCT15 or SW480 cells are treated with 25 μM KY1220 or KYA1797K or with control (DMSO) for 72 h. Cell proliferation is measured using the MTT assay. The absorbance of the formazan product is determined at 590 nm every 24 h[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Keywords:

    KY1220KY 1220KY-1220Wntβ-cateninBeta cateninInhibitorinhibitorinhibit

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    Product name:
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    Cat. No.:
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