KY1220
Based on 3 publication(s) in Google Scholar
KY1220 is a compound that destabilizes both β-catenin and Ras, via targeting the Wnt/β-catenin pathway, with an IC50 of 2.1 μM in HEK293 reporter cells.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 292168-79-7
- Formula: C14H10N4O3S
- Molecular Weight:314.32
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) KY1220
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Customer Validation & Images
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Biological Activity
IC50: 2.1 μM (HEK293 reporter cells)[1]
KY1220 shows an IC50 of 2.1 μM in HEK293 reporter cells. KY1220 dose dependently decreases Wnt3a-CM-induced TOPflash reporter activation and mRNA expression of Wnt target genes CCND1 and MYC in HEK293 cells. In HEK293 cells, both β-catenin and panRas protein levels are similarly reduced in a dose-dependent manner after treatment with KY1220, whereas the mRNA levels of CTNNB1 (which encodes β-catenin), NRAS, KRAS and HRAS remain unchanged. K-Ras, which has a critical role in progression of CRCs, is also destabilized by KY1220 via polyubiquitin-dependent proteasomal degradation. KY1220 accelerates the degradation rates of both β-catenin and Ras in SW480 cell lines. Ras destabilization by KY1220 consequently inhibits the activities of both ERK and Akt, which are downstream effectors of Ras in SW480 cells harboring a KRAS mutation. The proliferation and transformation of the HCT15, SW480, D-WT and D-MT CRC cells are efficiently inhibited after treatment with KY1220[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 292168-79-7
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Appearance Solid
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Molecular Weight 314.32
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Formula C14H10N4O3S
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Color Light yellow to yellow
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SMILES
O=C(/C(N1)=C/C2=CC=CN2C3=CC=C([N+]([O-])=O)C=C3)NC1=S
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (3)
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Journal Impact Factor
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Most Recent
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Cancer Lett
HDAC1-induced epigenetic silencing of ASPP2 promotes cell motility, tumour growth and drug resistance in renal cell carcinoma. [Abstract]2018 Sep 28:432:121-131. PMID: 29890207
KY1220 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2018 Sep 28:432:121-131. [Abstract]
The capability of ASPP2 in regulating epithelial–mesenchymal transition (EMT) is completely abolished by specific Wnt/β-catenin inhibitor KY1220.
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Life Sci
Cryptochrome 1 promotes osteogenic differentiation of human osteoblastic cells via Wnt/β-Catenin signaling. [Abstract]2018 Nov 1:212:129-137. PMID: 30290183
KY1220 purchased from MedChemExpress. Usage Cited in: Life Sci. 2018 Nov 1:212:129-137. [Abstract]
KY1220 reverses the increasing expression of β-Catenin after upregulating Cry1 (P<0.05).
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Biomed Res Int
Meta-Analysis of Mechanism of Influence of CRY2 on the Differentiation of Mouse Osteoblast through the Regulation of Wnt/ Β-Catenin Signaling Pathway. [Abstract]2022 Aug 21:2022:3727165. PMID: 36046448
Solvent & Solubility
DMSO : 50 mg/mL (159.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
HCT15 or SW480 cells are treated with 25 μM KY1220 or KYA1797K or with control (DMSO) for 72 h. Cell proliferation is measured using the MTT assay. The absorbance of the formazan product is determined at 590 nm every 24 h[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1815 mL | 15.9074 mL | 31.8147 mL | 79.5368 mL |
| 5 mM | 0.6363 mL | 3.1815 mL | 6.3629 mL | 15.9074 mL | |
| 10 mM | 0.3181 mL | 1.5907 mL | 3.1815 mL | 7.9537 mL | |
| 15 mM | 0.2121 mL | 1.0605 mL | 2.1210 mL | 5.3025 mL | |
| 20 mM | 0.1591 mL | 0.7954 mL | 1.5907 mL | 3.9768 mL | |
| 25 mM | 0.1273 mL | 0.6363 mL | 1.2726 mL | 3.1815 mL | |
| 30 mM | 0.1060 mL | 0.5302 mL | 1.0605 mL | 2.6512 mL | |
| 40 mM | 0.0795 mL | 0.3977 mL | 0.7954 mL | 1.9884 mL | |
| 50 mM | 0.0636 mL | 0.3181 mL | 0.6363 mL | 1.5907 mL | |
| 60 mM | 0.0530 mL | 0.2651 mL | 0.5302 mL | 1.3256 mL | |
| 80 mM | 0.0398 mL | 0.1988 mL | 0.3977 mL | 0.9942 mL | |
| 100 mM | 0.0318 mL | 0.1591 mL | 0.3181 mL | 0.7954 mL |
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