2. Membrane Transporter/Ion Channel Neuronal Signaling
  3. Calcium Channel
  4. NecroX-5

NecroX-5 is a derivative of the NecroX, reduces intracellular calcium concentration, and possesses anti-inflammatory and anti-cancer activity.

For research use only. We do not sell to patients.

NecroX-5 Chemical Structure

NecroX-5 Chemical Structure

CAS No. : 1383718-29-3

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 327 In-stock
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10 mM * 1 mL in DMSO USD 327 In-stock
Solid
5 mg USD 230 In-stock
10 mg USD 330 In-stock
25 mg USD 530 In-stock
50 mg USD 750 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

NecroX-5 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE NecroX-5

WB

    NecroX-5 purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2020 Apr;81:106257.  [Abstract]

    NecroX-5 inhibits the NLRP3 inflammasome activation in macrophages. RAW264.7 cells are firstly pretreated with 20 μM NecroX-5 for 1 h and then stimulated with LPS (1 μg/mL) for 8 h followed by 5 mmol/L of ATP for 30 min. Quantification of western blot in panen D.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    NecroX-5 is a derivative of the NecroX, reduces intracellular calcium concentration, and possesses anti-inflammatory and anti-cancer activity.

    IC50 & Target

    Calcium channel[1]
    In Vitro

    NecroX-5 (10 or 40 μM) inhibits breast cancer cell migration, which is correlated with mitoROS reduction, and mediated by AKT inhibition. NecroX-5 (10 μM) decreases intracellular calcium concentration in 4T1 cells by blocking Ca2+ influx, which mediated the inhibition of cell migration, AKT downregulation and the reduction of mitochondrial ROS levels[1]. NecroX-5 (10 µM) treatment for 24 h, effectively decreases the increased TNFα, TGFβ1, pSmad2 and the expression of Dcn in LPS-stimulated H9C2 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    NecroX-5 Related Antibodies
    In Vivo

    NecroX-5 (2.5 mg/kg, everyday other day) inhibits breast cancer cell metastasis in TUBO-P2J tumour-bearing mice[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    645.81

    Formula

    C27H39N3O9S3
    CAS No.
    Appearance

    Solid

    Color

    Light brown to brown

    SMILES

    O=S1(CCN(CC2=CC3=C(NC(C4=CC=CC=C4)=C3)C(NCC5CCOCC5)=C2)CC1)=O.CS(=O)(O)=O.CS(=O)(O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : 170 mg/mL (263.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5484 mL 7.7422 mL 15.4844 mL
    5 mM 0.3097 mL 1.5484 mL 3.0969 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.83 mg/mL (4.38 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.83 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (28.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.83 mg/mL (4.38 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.83 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (28.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.5484 mL 7.7422 mL 15.4844 mL 38.7111 mL
    5 mM 0.3097 mL 1.5484 mL 3.0969 mL 7.7422 mL
    10 mM 0.1548 mL 0.7742 mL 1.5484 mL 3.8711 mL
    15 mM 0.1032 mL 0.5161 mL 1.0323 mL 2.5807 mL
    20 mM 0.0774 mL 0.3871 mL 0.7742 mL 1.9356 mL
    25 mM 0.0619 mL 0.3097 mL 0.6194 mL 1.5484 mL
    30 mM 0.0516 mL 0.2581 mL 0.5161 mL 1.2904 mL
    40 mM 0.0387 mL 0.1936 mL 0.3871 mL 0.9678 mL
    50 mM 0.0310 mL 0.1548 mL 0.3097 mL 0.7742 mL
    60 mM 0.0258 mL 0.1290 mL 0.2581 mL 0.6452 mL
    80 mM 0.0194 mL 0.0968 mL 0.1936 mL 0.4839 mL
    100 mM 0.0155 mL 0.0774 mL 0.1548 mL 0.3871 mL
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    NecroX-5 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    NecroX-51383718-29-3NecroX5NecroX 5Calcium ChannelCa2+ channelsCa channelsInhibitorinhibitorinhibit

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