1. Protein Tyrosine Kinase/RTK
    JAK/STAT Signaling
  2. EGFR
  3. RG14620

RG14620 (Synonyms: Tyrphostin RG14620)

Cat. No.: HY-101426 Purity: 99.85%
Handling Instructions

RG14620 is an EGFR inhibitor with an IC50 of 3 μM.

For research use only. We do not sell to patients.

RG14620 Chemical Structure

RG14620 Chemical Structure

CAS No. : 136831-49-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 61 In-stock
Estimated Time of Arrival: December 31
5 mg USD 55 In-stock
Estimated Time of Arrival: December 31
10 mg USD 80 In-stock
Estimated Time of Arrival: December 31
50 mg USD 300 In-stock
Estimated Time of Arrival: December 31
100 mg USD 500 In-stock
Estimated Time of Arrival: December 31
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500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

RG14620 is an EGFR inhibitor with an IC50 of 3 μM.

IC50 & Target[1]

EGFR

3 μM (IC50, Cell Assay)

In Vitro

RG14620 inhibits colony formation (IC50=3 μM) and DNA synthesis (IC50=1 μM) by HER 14 cells, which are stimulated by 50 ng/mL EGF, in a dose-dependent manner. RG14620 also suppresses colony formation(IC50=4 μM) and DNA synthesis (IC50=1.25 μM) by EGF-stimulated MH-85 cells in a dose-dependent manner. The growth-inhibitory effect of RG14620 irreversible[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

RG14620, at a dose of 200 g/mouse/day inhibits H-85 tumor growth in nude mice. Mice show less cachexia and hypercalcemia, eat more food, and are more active than untreated MH-85 tumor-bearing animals[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

275.13

Formula

C₁₄H₈Cl₂N₂

CAS No.

136831-49-7

SMILES

N#C/C(C1=CC=CN=C1)=C/C2=CC(Cl)=CC(Cl)=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (121.14 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6346 mL 18.1732 mL 36.3465 mL
5 mM 0.7269 mL 3.6346 mL 7.2693 mL
10 mM 0.3635 mL 1.8173 mL 3.6346 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.5 mg/mL (9.09 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (9.09 mM); Suspended solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (9.09 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[2]

MH-85 cells and HER 14 cells are plated in complete medium, either αMEM or DMEM, respectively, supplemented with 10% FCS. After overnight culture, the culture medium is switched to αMEM supplemented with 0.2% PCS and 50 ng/mL EGF (MH-85) or DMEM supplemented with 0.5% PCS and 50 ng/mL EGF (HER14). The cells are cultured in this medium in the presence or absence of increasing concentrations of RG-13022 or RG-14620 for 10 days. At the end of culture, the cells are fixed with 4% (v/v) formaldehyde in calcium-magnesium-free phosphate-buffered saline for 15 min at room temperature and stained with hematoxylin. Numbers of colonies including more than 20 cells in each well are counted under the microscope[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice: RG14620 in 0.1 mL 100% DMSO is injected i.p. twice a day from 1day after MH-85 tumor inoculation. Control animals are given the same vehicle[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

RG14620Tyrphostin RG14620RG 14620RG-14620Tyrphostin RG 14620Tyrphostin RG-14620EGFREpidermal growth factor receptorErbB-1HER1Inhibitorinhibitorinhibit

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RG14620
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HY-101426
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