2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. TLR7 agonist 1

TLR7 agonist 1 is a selective TLR7 agonist with an LEC value of 90 nM. TLR7 agonist 1 activates downstream immune regulation and shows no activity against TLR8. TLR7 agonist 1 induces endogenous IFN-α production in mice.

For research use only. We do not sell to patients.

TLR7 agonist 1

TLR7 agonist 1 Chemical Structure

CAS No. : 2178156-33-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

TLR7 agonist 1 Related Antibodies

Top Publications Citing Use of Products

View All Toll-like Receptor (TLR) Isoform Specific Products:

View All Isoforms
TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

TLR7 agonist 1 is a selective TLR7 agonist with an LEC value of 90 nM. TLR7 agonist 1 activates downstream immune regulation and shows no activity against TLR8. TLR7 agonist 1 induces endogenous IFN-α production in mice[1].

IC50 & Target[1]

TLR7

 

Cellular Effect
Cell Line Type Value Description References
HEK293 CC50
> 25 μM
Compound: 48
Cytotoxic activity against HEK293 cells assessed as reduction in cell viability incubated for 6 hrs
Cytotoxic activity against HEK293 cells assessed as reduction in cell viability incubated for 6 hrs
[PMID: 29366653]
In Vitro

TLR7 agonist 1 (compound 48) (0.09 μM) is a highly potent, TLR7-selective agonist (SI >200 over TLR8) in HEK-293 cells transiently transfected with human TLR7, with an hTLR7 LEC of 0.09 μM[1].
TLR7 agonist 1 (0.14 μM) induces IFN-α in hPBMCs with an LEC50 of 0.14 μM[1].
TLR7 agonist 1 (>25 μM) has low cytotoxicity in HEK-293 cells[1].
TLR7 agonist 1 (1 μM; 15 min) exhibits moderate metabolic stability in human liver microsomes[1].
TLR7 agonist 1 (10 μM) shows no CYP inhibition up to 10 μM in human liver microsomes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

TLR7 agonist 1 Related Antibodies
Parmacokinetics
Species Dose Route Tmax Cmax AUC0-last
Rat[1] 5 mg/kg p.o. 1 h 142 ng/mL 365 ng·h/mL
In Vivo

TLR7 agonist 1 (compound 48) (5 mg/kg; p.o.; single dose) induces a peak plasma mIFN-α level of 2800 pg/mL at 4 hours in healthy C57BL/6 mice, with higher liver exposure than systemic exposure[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

363.46

Formula

C21H25N5O
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

NC1=NC2=C(C(C(N[C@@H](C)C3=CC=CC=C3)=O)=CC=C2)C(NCCCC)=N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (275.13 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7513 mL 13.7567 mL 27.5133 mL
5 mM 0.5503 mL 2.7513 mL 5.5027 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7513 mL 13.7567 mL 27.5133 mL 68.7834 mL
5 mM 0.5503 mL 2.7513 mL 5.5027 mL 13.7567 mL
10 mM 0.2751 mL 1.3757 mL 2.7513 mL 6.8783 mL
15 mM 0.1834 mL 0.9171 mL 1.8342 mL 4.5856 mL
20 mM 0.1376 mL 0.6878 mL 1.3757 mL 3.4392 mL
25 mM 0.1101 mL 0.5503 mL 1.1005 mL 2.7513 mL
30 mM 0.0917 mL 0.4586 mL 0.9171 mL 2.2928 mL
40 mM 0.0688 mL 0.3439 mL 0.6878 mL 1.7196 mL
50 mM 0.0550 mL 0.2751 mL 0.5503 mL 1.3757 mL
60 mM 0.0459 mL 0.2293 mL 0.4586 mL 1.1464 mL
80 mM 0.0344 mL 0.1720 mL 0.3439 mL 0.8598 mL
100 mM 0.0275 mL 0.1376 mL 0.2751 mL 0.6878 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

TLR7 agonist 1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TLR7 agonist 12178156-33-5Toll-like Receptor (TLR)hPBMCsC57BL/6 micemonkey TLR7miceTLR8Toll-like receptor 7interferon-alphahuman TLR7HEK-293 cellshuman liver microsomesInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
TLR7 agonist 1
Cat. No.:
HY-111358
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (251 KB) Français - FR (251 KB) Deutsch - DE (251 KB) Norwegian - NO (251 KB) Español - ES (251 KB) Swedish - SV (251 KB) Italian - IT (251 KB) Korean - KR (251 KB) Portuguese - PT (251 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.