1. Membrane Transporter/Ion Channel Neuronal Signaling
  2. Calcium Channel
  3. Trimethadione

Trimethadione  (Synonyms: 3,5,5,-Trimethyloxazolidine-2,4-dione)

Cat. No.: HY-A0092 Purity: 98.77%
Handling Instructions Technical Support

Trimethadione (3,5,5,-Trimethyloxazolidine-2,4-dione) is an oxazolidinedione anticonvulsant agent widely used against absences seizures. Trimethadione also is a T-type calcium channel blocker which has antihyperalgesic effects.

For research use only. We do not sell to patients.

Trimethadione Chemical Structure

Trimethadione Chemical Structure

CAS No. : 127-48-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Trimethadione:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Trimethadione (3,5,5,-Trimethyloxazolidine-2,4-dione) is an oxazolidinedione anticonvulsant agent widely used against absences seizures. Trimethadione also is a T-type calcium channel blocker which has antihyperalgesic effects[1][2].

IC50 & Target

T-type calcium channel

 

In Vivo

Trimethadione (100-600 mg/kg, ip, single dose) ameliorates Capsaicin (HY-10448)-induced mechanical hyperalgesia in rats, exhibits anticonvulsant effect in rat pentylenetetrazol-induced epilepsy model[2].
Trimethadione (200 mg/kg/day, po, in drinking water for 2-3 weeks) prevents and reduces the permanent hearing loss (PTS) induced by noise, ameliorates the temporary hearing loss (TTS) in C57BL/6J mouse models[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Capsaicin (HY-10448)-induced rats mechanical hyperalgesia model[2]
Dosage: 100-600 mg/kg
Administration: ip, single dose
Result: Reversed capsaicin-induced mechanical hyperalgesia.
Animal Model: C57BL/6J mouse models[3]
Dosage: 200 mg/kg/day
Administration: po, in drinking water for 2-3 weeks
Result: Reduced noise-induced hearing loss.
Molecular Weight

143.14

Synonyms

3,5,5,-Trimethyloxazolidine-2,4-dione

Formula

C6H9NO3

CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C(N1C)OC(C)(C)C1=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (698.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.9861 mL 34.9303 mL 69.8607 mL
5 mM 1.3972 mL 6.9861 mL 13.9721 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (17.47 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (17.47 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.77%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.9861 mL 34.9303 mL 69.8607 mL 174.6517 mL
5 mM 1.3972 mL 6.9861 mL 13.9721 mL 34.9303 mL
10 mM 0.6986 mL 3.4930 mL 6.9861 mL 17.4652 mL
15 mM 0.4657 mL 2.3287 mL 4.6574 mL 11.6434 mL
20 mM 0.3493 mL 1.7465 mL 3.4930 mL 8.7326 mL
25 mM 0.2794 mL 1.3972 mL 2.7944 mL 6.9861 mL
30 mM 0.2329 mL 1.1643 mL 2.3287 mL 5.8217 mL
40 mM 0.1747 mL 0.8733 mL 1.7465 mL 4.3663 mL
50 mM 0.1397 mL 0.6986 mL 1.3972 mL 3.4930 mL
60 mM 0.1164 mL 0.5822 mL 1.1643 mL 2.9109 mL
80 mM 0.0873 mL 0.4366 mL 0.8733 mL 2.1831 mL
100 mM 0.0699 mL 0.3493 mL 0.6986 mL 1.7465 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Trimethadione
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