Trimethadione
Based on 1 Customer Validation
Trimethadione (3,5,5,-Trimethyloxazolidine-2,4-dione) is an oxazolidinedione anticonvulsant agent widely used against absences seizures. Trimethadione also is a T-type calcium channel blocker which has antihyperalgesic effects.
For research use only. We do not sell to patients.
- Purity: 99.59%
- CAS No.: 127-48-0
- Formula: C6H9NO3
- Molecular Weight:143.14
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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All Calcium Channel Isoforms
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Biological Activity
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T-type calcium channel |
Trimethadione (200 mg/kg/day, po, in drinking water for 2-3 weeks) prevents and reduces the permanent hearing loss (PTS) induced by noise, ameliorates the temporary hearing loss (TTS) in C57BL/6J mouse models[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Capsaicin (HY-10448)-induced rats mechanical hyperalgesia model[2]
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Dosage:100-600 mg/kg
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Administration:ip, single dose
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Result:Reversed capsaicin-induced mechanical hyperalgesia.
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Animal Model:C57BL/6J mouse models[3]
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Dosage:200 mg/kg/day
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Administration:po, in drinking water for 2-3 weeks
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Result:Reduced noise-induced hearing loss.
Chemical Information
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CAS No. 127-48-0
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Appearance Solid
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Molecular Weight 143.14
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Formula C6H9NO3
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Color White to light yellow
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SMILES
O=C(N1C)OC(C)(C)C1=O
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Synonyms
3,5,5,-Trimethyloxazolidine-2,4-dione
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 100 mg/mL (698.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (17.47 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (17.47 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (557 KB)
- English - EN (557 KB)
- Français - FR (557 KB)
- Deutsch - DE (557 KB)
- Norwegian - NO (557 KB)
- Español - ES (557 KB)
- Swedish - SV (557 KB)
- Italian - IT (557 KB)
- Korean - KR (557 KB)
- Portuguese - PT (557 KB)
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Handling Instructions (2659 KB)
References
[1]. Pastore V, et, al. Synthesis and anticonvulsant activity of bioisosteres of trimethadione, N-derivative-1,2,3-oxathiazolidine-4-one-2,2-dioxides from α-hydroxyamides. Bioorg Med Chem. 2013 Feb 15;21(4):841-6. [Content Brief]
[2]. Barton ME, et, al. The antihyperalgesic effects of the T-type calcium channel blockers ethosuximide, trimethadione, and mibefradil. Eur J Pharmacol. 2005 Oct 3;521(1-3):79-85. [Content Brief]
[3]. Shen H, et al., Prophylactic and therapeutic functions of T-type calcium blockers against noise-induced hearing loss. Hear Res. 2007 Apr;226(1-2):52-60. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 6.9861 mL | 34.9303 mL | 69.8607 mL | 174.6517 mL |
| 5 mM | 1.3972 mL | 6.9861 mL | 13.9721 mL | 34.9303 mL | |
| 10 mM | 0.6986 mL | 3.4930 mL | 6.9861 mL | 17.4652 mL | |
| 15 mM | 0.4657 mL | 2.3287 mL | 4.6574 mL | 11.6434 mL | |
| 20 mM | 0.3493 mL | 1.7465 mL | 3.4930 mL | 8.7326 mL | |
| 25 mM | 0.2794 mL | 1.3972 mL | 2.7944 mL | 6.9861 mL | |
| 30 mM | 0.2329 mL | 1.1643 mL | 2.3287 mL | 5.8217 mL | |
| 40 mM | 0.1747 mL | 0.8733 mL | 1.7465 mL | 4.3663 mL | |
| 50 mM | 0.1397 mL | 0.6986 mL | 1.3972 mL | 3.4930 mL | |
| 60 mM | 0.1164 mL | 0.5822 mL | 1.1643 mL | 2.9109 mL | |
| 80 mM | 0.0873 mL | 0.4366 mL | 0.8733 mL | 2.1831 mL | |
| 100 mM | 0.0699 mL | 0.3493 mL | 0.6986 mL | 1.7465 mL |
Trimethadione Related Classifications
HY-A0092 Related Classifications
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