Acetazolamide sodium
Based on 16 publication(s) in Google Scholar
Acetazolamide sodium is a carbonic anhydrase (CA) IX inhibitor with an IC50 of 30 nM for hCA IX. Acetazolamide sodium has diuretic, antihypertensive and anti-gonococcal activities.
For research use only. We do not sell to patients.
- Purity : 99.86%
- CAS No.: 1424-27-7
- Formula: C4H6N4NaO3S2
- Molecular Weight:245.24
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-B0553
MethazolamideCarbonic Anhydrase Inhibitor -
HY-B0782
AcetazolamideCA IX Inhibitor -
HY-13513
U-104CA IX/XII Inhibitor -
HY-B0483
TioxoloneCarbonic Anhydrase Inhibitor -
HY-B0782R
Acetazolamide (Standard) -
HY-B0588
BrinzolamideCarbonic Anhydrase II Inhibitor -
HY-B0109A
Dorzolamide hydrochlorideCarbonic Anhydrase II Inhibitor -
HY-101087
BenzenesulphonamideCA Inhibitor -
HY-B0397
DichlorphenamideCarbonic Anhydrase Inhibitor -
HY-13650
IndisulamCarbonic Anhydrase Inhibitor/RBM39 Molecular Glue Degrader -
HY-B0122
TopiramateAntiepileptic Agent -
HY-B0483R
Tioxolone (Standard)Carbonic Anhydrase Inhibitor
Publications Citing Use of MedChemExpress (MCE) Acetazolamide sodium
More- Cell Rep Med. 2026 Jul 13:102920.
- ACS Environ Au. 2025 Aug 5;5(6):573-582. [Abstract]
- Antioxidants (Basel). 2026 Apr 22;15(5):525. [Abstract]
- Antioxidants (Basel). 2024 Apr 17;13(4):473. [Abstract]
- Cell Rep. 2023 May 17;42(5):112529. [Abstract]
- Anal Chem. 2025 Jun 3;97(21):11099-11109. [Abstract]
- Anal Chem. 2020 Jun 2;92(11):7657-7665. [Abstract]
- Plant Cell Environ. 2025 Sep;48(9):6704-6721. [Abstract]
- Int Immunopharmacol. 2025 Jun 17:158:114845. [Abstract]
- FASEB J. 2026 Jun 15;40(11):e71960. [Abstract]
- J Biol Chem. 2025 Mar;301(3):108303. [Abstract]
- J Pharm Biomed Anal. 2021 Feb 20:195:113870. [Abstract]
- Biochem Biophys Res Commun. 2025 Sep 19:785:152676. [Abstract]
- bioRxiv. 2023 Sep 8.
- Research Square Print. December 16th, 2022.
- Elife. 2018 Feb 2:7:e33432. [Abstract]
Customer Validation & Images
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WB
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
Biological Activity
Description
IC50 & Target
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CA IX |
In Vitro
Acetazolamide also inhibits hCA II with an IC50 of 130 nM[1].
Acetazolamide (Ace) is a small heteroaromatic sulfonamide that binds to various carbonic anhydrases with high affinity, acting as a carbonic anhydrase (CA) inhibitor[2].
Compared with the control group, the high Acetazolamide concentration (AceH, 50 nM), Cisplatin (Cis; 1 μg/mL) and Cis combined with the low Acetazolamide concentration (AceL, 10 nM) treatments significantly reduces viability of Hep-2 cells[2].
Treatment with the Acetazolamide/Cis combination significantly increases the expression levels of P53, as both AceL+Cis and AceH+Cis treatments result in significantly increased P53 protein expression levels compared with the control group. The Ace/Cis combination treatment significantly reduces the bcl-2/bax expression ratio, and increases the expression of caspase-3 protein, compared with the control group. AceL, AceH, Cis and AceL+Cis treatments significantly reduce the bcl-2/bax ratio compared with the control group[2].
Combined Ace and Cis treatment effectively promotes apoptosis in Hep-2 cells[2].
Combined treatment with Ace/Cis markedly decreases the expression of AQP1 mRNA in Hep-2 cells. Both AceH and AceL+Cis treatments decrease the expression of aquaporin-1 (AQP1) mRNA in Hep-2 cells compared with the control group[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
Acetazolamide (40 mg/kg) and/or MS-275 treatment reduce expression of HIF1-α and CAIX in NB SH-SY5Y xenograft[3].
Acetazolamide (40 mg/kg), MS-275 and Acetazolamide+MS-275 reduce expression of mitotic and proliferative markers in NB SH-SY5Y xenografts[3]. Acetazolamide (50 mg/kg; PO, for 3 days) significantly reduces the gonococcal load in the vagina of infected mice by 90%[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1424-27-7
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Appearance Solid
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Molecular Weight 245.24
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Formula C4H6N4NaO3S2
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Color White to off-white
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SMILES
CC(NC1=NN=C(S(=O)(N)=O)S1)=O.[Na]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (16)
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Journal Impact Factor
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Most Recent
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ACS Environ Au
Machine Learning-Assisted Recognition of Environmental Sulfur-Containing Chemicals in Nontargeted Mass Spectrometry Analysis of Inadequate Mass Resolution. [Abstract]2025 Aug 5;5(6):573-582. PMID: 41277996 -
Antioxidants (Basel)
Thioamide Compound H0802 Enhances Hypoxia Tolerance by Mimicking Hypoxia-Adaptive Reprogramming of Glucose and Oxygen Metabolism. [Abstract]2026 Apr 22;15(5):525. PMID: 42193148 -
Antioxidants (Basel)
Redox Regulation of Phosphatase and Tensin Homolog by Bicarbonate and Hydrogen Peroxide: Implication of Peroxymonocarbonate in Cell Signaling. [Abstract]2024 Apr 17;13(4):473. PMID: 38671920
Acetazolamide sodium purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2024 Apr 17;13(4):473. [Abstract]
Effect of Acetazolamide, a CA inhibitor, on oxidation of PTEN by H2O2. HepG2 cells were pre-incubated in HCO3−-free media, supplemented with 0, 0.01, 0.1, 1, and 10 µM Acetazolamide, at 37 °C with 0.1% CO2 for 4 h. Then, they were changed to stimulation media with same components plus 0.5 mM H2O2. After 10 min, the reaction was terminated by NEM-containing lysis buffer. Western blot data with PTEN and β-actin antibodies are shown Acetazolamide considerably impeded PTEN oxidation in a dose-dependent manner.
Acetazolamide sodium purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2024 Apr 17;13(4):473. [Abstract]
Effect of Acetazolamide, a CA inhibitor, on oxidation of PTEN by H2O2. HepG2 cells were pre-incubated in HCO3−-free media, supplemented with 0, 0.01, 0.1, 1, and 10 µM Acetazolamide, at 37 °C with 0.1% CO2 for 4 h. Then, they were changed to stimulation media with same components plus 0.5 mM H2O2. After 10 min, the reaction was terminated by NEM-containing lysis buffer. Western blot data with PTEN and β-actin antibodies are shown. Stars are considered as significances.
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Cell Rep
Architecture of androgen receptor pathways amplifying glucagon-like peptide-1 insulinotropic action in male pancreatic β cells. [Abstract]2023 May 17;42(5):112529. PMID: 37200193
Acetazolamide sodium purchased from MedChemExpress. Usage Cited in: Cell Rep. 2023 May 17;42(5):112529. [Abstract]
GSIS was measured via static incubation in male human islets (n = 3–7 donors, 10 IEQ/condition measured in triplicate) cultured for 24 h with vehicle or DHT (10 nM) and then for 40 min in the presence or absence of a carbonic anhydrase inhibitor Acetazolamide (50 μM).
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Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576 -
Anal Chem
Untargeted Defining Protein-Metabolites Interaction Based on Label-Free Kinetic Size Exclusion Chromatography-Mass Spectrometry. [Abstract]2020 Jun 2;92(11):7657-7665. PMID: 32384235 -
Plant Cell Environ
Gα Protein Mediates High-Concentration CO2-Induced Stomata Closure Through Interaction With Carbonic Anhydrase to Promote Guard Cell Hydrogen Peroxide Production in Arabidopsis. [Abstract]2025 Sep;48(9):6704-6721. PMID: 40421746 -
Int Immunopharmacol
Acetazolamide as an aquaporin 1 inhibitor mitigates rheumatoid arthritis by reducing angiogenesis via the modulation of the FAK-PI3K/Akt signaling pathway. [Abstract]2025 Jun 17:158:114845. PMID: 40359885 -
FASEB J
1-Naphthol Drives Liver Steatosis and Fibrosis via the CA3-CD36 Axis Independent of Obesity. [Abstract]2026 Jun 15;40(11):e71960. PMID: 42201771 -
J Biol Chem
Aquaporin 5 deficiency suppresses fatty acid oxidation and delays liver regeneration through the transcription factor PPAR. [Abstract]2025 Mar;301(3):108303. PMID: 39947476 -
J Pharm Biomed Anal
Screening method of mildronate and over 300 doping agents by reversed-phase liquid chromatography-high resolution mass spectrometry. [Abstract]2021 Feb 20:195:113870. PMID: 33453569 -
Biochem Biophys Res Commun
Glucose transports in the ileum: mechanism, regulation and physiological role of ileal glucose absorption. [Abstract]2025 Sep 19:785:152676. PMID: 41005286 -
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Elife
Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility. [Abstract]2018 Feb 2:7:e33432. PMID: 29393851
Acetazolamide sodium purchased from MedChemExpress. Usage Cited in: Elife. 2018 Feb 2:7:e33432. [Abstract]
Intracellular pH (pHi) of the ligated efferent ductules with or without Acetazolamide(500 μM) from WT mice and Adgrg2-/Y mice.
Solvent & Solubility
In Vitro:
H2O : ≥ 100 mg/mL (407.76 mM)
DMSO : 100 mg/mL (407.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
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Data Sheet (287 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Bayat Mokhtari R, et al. Acetazolamide potentiates the anti-tumor potential of HDACi, MS-275, in neuroblastoma. BMC Cancer. 2017 Feb 24;17(1):156. [Content Brief]
[2]. Hou Z, et al. Dual-tail approach to discovery of novel carbonic anhydrase IX inhibitors by simultaneously matching the hydrophobic and hydrophilic halves of the active site. Eur J Med Chem. 2017 May 26;132:1-10. [Content Brief]
[3]. Gao H, et al. Combined treatment with acetazolamide and cisplatin enhances chemosensitivity in laryngeal carcinoma Hep-2 cells. Oncol Lett. 2018 Jun;15(6):9299-9306. [Content Brief]
[4]. Kassamali R, et al. Acetazolamide: a forgotten diuretic agent. Cardiol Rev. 2011 Nov-Dec;19(6):276-8. [Content Brief]
[5]. Jabeen E, et al. Interaction of antihypertensive acetazolamide with nonsteroidal anti-inflammatory drugs. J Photochem Photobiol B. 2013 Aug 5;125:155-63. [Content Brief]
[6]. Abutaleb NS, et al. In vivo efficacy of acetazolamide in a mouse model of Neisseria gonorrhoeae infection. Microb Pathog. 2022 Mar;164:105454. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 4.0776 mL | 20.3882 mL | 40.7764 mL | 101.9410 mL |
| 5 mM | 0.8155 mL | 4.0776 mL | 8.1553 mL | 20.3882 mL | |
| 10 mM | 0.4078 mL | 2.0388 mL | 4.0776 mL | 10.1941 mL | |
| 15 mM | 0.2718 mL | 1.3592 mL | 2.7184 mL | 6.7961 mL | |
| 20 mM | 0.2039 mL | 1.0194 mL | 2.0388 mL | 5.0970 mL | |
| 25 mM | 0.1631 mL | 0.8155 mL | 1.6311 mL | 4.0776 mL | |
| 30 mM | 0.1359 mL | 0.6796 mL | 1.3592 mL | 3.3980 mL | |
| 40 mM | 0.1019 mL | 0.5097 mL | 1.0194 mL | 2.5485 mL | |
| 50 mM | 0.0816 mL | 0.4078 mL | 0.8155 mL | 2.0388 mL | |
| 60 mM | 0.0680 mL | 0.3398 mL | 0.6796 mL | 1.6990 mL | |
| 80 mM | 0.0510 mL | 0.2549 mL | 0.5097 mL | 1.2743 mL | |
| 100 mM | 0.0408 mL | 0.2039 mL | 0.4078 mL | 1.0194 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Acetazolamide sodium Related Classifications
HY-B0782A Related Classifications
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