Actithiazic acid 

Actithiazic acid is a thiazolidinone antibiotic that targets biotin synthase. Actithiazic acid interferes with essential bacterial metabolism by inhibiting the final step of biotin synthesis (conversion of desthiobiotin to biotin, IC₅₀ = 0.45 μM). Actithiazic acid can be used in studies related to mycobacterial infections^[1][2].

Actithiazic acid (0.45-3.9 μM; 20 min) inhibits the enzymatic conversion of desthiobiotin to biotin in resting cell suspensions of Escherichia coli K-12 strain ABD313-136, with an IC₅₀ of 0.45 μM^[1].
Actithiazic acid (1 μg/mL) induces derepression of the biotin A operon in Escherichia coli K-12 strain Y10-1, with the maximum enhancement of 7,8-diaminopelargonic acid aminotransferase and desthiobiotin synthetase activities observed at the concentration of 1 μg/mL^[1].
Actithiazic acid promotes the excretion of biotin-like substances in growth cultures of Escherichia coli and various other microorganisms^[1].
Combination of actithiazic acid (10-50 μg/mL; 18 h) with Cephalexin (HY-B0200) exerts moderate synergistic effects against *Escherichia coli* 9632 in nutrient broth^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

217.29

C₉H₁₅NO₃S

539-35-5

O=C(CCCCCC1SCC(N1)=O)O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Eisenberg MA, et al. Mode of action of the biotin antimetabolites actithiazic acid and alpha-methyldethiobiotin. Antimicrob Agents Chemother. 1982;21(1):5-10.

  [2]. Nadkarni SR, et al. In vitro synergy of actithiazic acid, an antimetabolite of biotin, combined with cephalexin. The Journal of Antibiotics. 1983;36(11):1567-8.