Anagyrine
Based on 1 Customer Validation
Anagyrine ((-)-Anagyrine) is a quinolizidine alkaloid that has been found in Lupinus albus. Anagyrine binds to muscarinic and nicotinic acetylcholine receptors with IC50 values of 132 and 2096 µM respectively. Anagyrine is a potent and effective desensitizer of nAChR, and Anagyrine can directly, without metabolism, desensitize nAChR.
For research use only. We do not sell to patients.
- Purity: 98.15%
- CAS No.: 486-89-5
- Formula: C15H20N2O
- Molecular Weight:244.33
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Anagyrine acts as a partial agonist in both cell lines with EC50 values of 4.2 and 231 μM in SH-SY5Y and TE-671 cells, respectively. Anagyrine is a desensitizer of nAChR with DC50 values of 6.9 and 139 μM in SH-SY5Y and TE-671 cells, respectively[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 486-89-5
-
Appearance Oil
-
Molecular Weight 244.33
-
Formula C15H20N2O
-
Color Light brown to brown
-
SMILES
O=C1N2C([C@]3([H])C[C@]([C@]4([H])N(CCCC4)C3)([H])C2)=CC=C1
-
Synonyms
(-)-Anagyrine; Monolupine; Rhombinine
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
H2O : 25 mg/mL (102.32 mM; Need ultrasonic)
DMSO : 25 mg/mL (102.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (8.19 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% Saline
Solubility: ≥ 2 mg/mL (8.19 mM); Clear solution
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
-
Data Sheet (277 KB)
-
SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
-
Handling Instructions (2659 KB)
References
[1]. Schmeller T, et al. Binding of quinolizidine alkaloids to nicotinic and muscarinic acetylcholine receptors. J Nat Prod. 1994 Sep;57(9):1316-9. [Content Brief]
[3]. Green BT, et al. Anagyrine desensitization of peripheral nicotinic acetylcholine receptors. A potential biomarker of quinolizidine alkaloid teratogenesis in cattle. Res Vet Sci. 2017 Dec;115:195-200. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 4.0928 mL | 20.4641 mL | 40.9283 mL | 102.3206 mL |
| 5 mM | 0.8186 mL | 4.0928 mL | 8.1857 mL | 20.4641 mL | |
| 10 mM | 0.4093 mL | 2.0464 mL | 4.0928 mL | 10.2321 mL | |
| 15 mM | 0.2729 mL | 1.3643 mL | 2.7286 mL | 6.8214 mL | |
| 20 mM | 0.2046 mL | 1.0232 mL | 2.0464 mL | 5.1160 mL | |
| 25 mM | 0.1637 mL | 0.8186 mL | 1.6371 mL | 4.0928 mL | |
| 30 mM | 0.1364 mL | 0.6821 mL | 1.3643 mL | 3.4107 mL | |
| 40 mM | 0.1023 mL | 0.5116 mL | 1.0232 mL | 2.5580 mL | |
| 50 mM | 0.0819 mL | 0.4093 mL | 0.8186 mL | 2.0464 mL | |
| 60 mM | 0.0682 mL | 0.3411 mL | 0.6821 mL | 1.7053 mL | |
| 80 mM | 0.0512 mL | 0.2558 mL | 0.5116 mL | 1.2790 mL | |
| 100 mM | 0.0409 mL | 0.2046 mL | 0.4093 mL | 1.0232 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Anagyrine Related Classifications
HY-121027 Related Classifications
Powered by Bioz