Antitrypanosomal agent 12
Antitrypanosomal agent 12 is a C20-phenylthiourea with trypanocidal and cytotoxic activities. Antitrypanosomal agent 12 shows antitrypanosomal activity with 50% growth inhibition (GI50) values of 0.22 μM. Antitrypanosomal agent 12 induces faster cell swelling in bloodstream-form trypanosomes than Salinomycin (HY-15597).
For research use only. We do not sell to patients.
- Formula: C49H77N2NaO10S
- Molecular Weight:909.20
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Biological Activity
Description
In Vitro
Antitrypanosomal agent 12 (compound 4d) (100 mM; 60 min) displays faster cell swelling activity than native Salinomycin in T. brucei bloodstream forms[1].
Antitrypanosomal agent 12 shows selectivity on T. brucei over HL-60 cells with MIC values of 1 μM (T. brucei), and 10 μM (HL-60 cells), respectivley[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Molecular Weight 909.20
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Formula C49H77N2NaO10S
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SMILES
CC[C@H]([C@@H]1[C@@H](C)C[C@@H](C)[C@@]2(CC[C@H](NC(NC3=CC=CC=C3)=S)[C@@]4(O[C@@]([C@@H]5O[C@@H](C)[C@@](O)(CC)CC5)(C)CC4)O2)O1)C([C@H]([C@@H](O)[C@H](C)[C@]6([H])O[C@@H]([C@H](C(O[Na])=O)CC)CC[C@@H]6C)C)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Antitrypanosomal agent 12 Related Classifications
HY-149985 Related Classifications
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