2. Protein Tyrosine Kinase/RTK Cell Cycle/DNA Damage Neuronal Signaling
  3. DYRK CDK Tau Protein Amyloid-β
  4. AO-365/43472821

AO-365/43472821 is a selective, brain-penetrant Dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A) inhibitor (IC50 = 0.29 μM) and shows a significant inhibitory effect on (CDC-like kinase 1) CLK1 (IC50 = 0.08 μM). AO-365/43472821 could protect the human neuroblastoma cell line SH-SY5Y from Okadaic acid (HY-N6785) (OA)-induced injury. AO-365/43472821 decreased tau (pSer396)/tau and Aβ1-42 protein expression. AO-365/43472821 can be used for the study of Alzheimer's disease.

For research use only. We do not sell to patients.

AO-365/43472821

AO-365/43472821 Chemical Structure

CAS No. : 1081122-55-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

AO-365/43472821 Related Antibodies

Top Publications Citing Use of Products

View All DYRK Isoform Specific Products:

View All Isoforms
DYRK1 DYRK2 DYRK3 DYRK4 HIPK PRP4

View All CDK Isoform Specific Products:

View All Isoforms
CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85 CDK10 CDK15 CDK17 CDK18 CDK20 PITSLRE

  • Biological Activity

  • Purity & Documentation

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Description

AO-365/43472821 is a selective, brain-penetrant Dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A) inhibitor (IC50 = 0.29 μM) and shows a significant inhibitory effect on (CDC-like kinase 1) CLK1 (IC50 = 0.08 μM). AO-365/43472821 could protect the human neuroblastoma cell line SH-SY5Y from Okadaic acid (HY-N6785) (OA)-induced injury. AO-365/43472821 decreased tau (pSer396)/tau and Aβ1-42 protein expression. AO-365/43472821 can be used for the study of Alzheimer's disease[1].

IC50 & Target

CLK1

0.08 μM (IC50)

DYRK1A

0.29 μM (IC50)

In Vitro

AO-365/43472821 (Compound Y16-5) (1 μM, 48 h) significantly protects SH-SY5Y cells from OA-induced damage and improves cell survival[1].
AO-365/43472821 (1-10 μM) significantly reduces the tau (pSer 396)/tau level in SH-SY5Y cells in a concentration-dependent manner, and also reduces the expression of Aβ1-42 protein[1].
AO-365/43472821 (48 h) shows a CC50 greater than 100 μM in both SH-SY5Y and HL-7702 cells, indicating no cytotoxicity[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AO-365/43472821 Related Antibodies

Cell Viability Assay[1]

Cell Line: SH-SY5Y cells with OA (100 nM) for 24 h
Concentration: 1 μM
Incubation Time: 48 h, incubate for 24 hours, then add OA and incubate for another 24 hours
Result: Protected SH-SY5Y cells from the OA-induced injury in a statistically significant way.
Molecular Weight

412.42

Formula

C19H16N4O5S
CAS No.
Appearance

Solid

Color

Off-white to light brown

SMILES

COC1=CC=CC=C1OCC2=NN3C(COC4=CC5=C(OCO5)C=C4)=NN=C3S2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (60.62 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4247 mL 12.1236 mL 24.2471 mL
5 mM 0.4849 mL 2.4247 mL 4.8494 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4247 mL 12.1236 mL 24.2471 mL 60.6178 mL
5 mM 0.4849 mL 2.4247 mL 4.8494 mL 12.1236 mL
10 mM 0.2425 mL 1.2124 mL 2.4247 mL 6.0618 mL
15 mM 0.1616 mL 0.8082 mL 1.6165 mL 4.0412 mL
20 mM 0.1212 mL 0.6062 mL 1.2124 mL 3.0309 mL
25 mM 0.0970 mL 0.4849 mL 0.9699 mL 2.4247 mL
30 mM 0.0808 mL 0.4041 mL 0.8082 mL 2.0206 mL
40 mM 0.0606 mL 0.3031 mL 0.6062 mL 1.5154 mL
50 mM 0.0485 mL 0.2425 mL 0.4849 mL 1.2124 mL
60 mM 0.0404 mL 0.2021 mL 0.4041 mL 1.0103 mL
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AO-365/43472821 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AO-365/434728211081122-55-5DYRKCDKTau ProteinAmyloid-βDual specificity tyrosine phosphorylation regulated kinaseDual specificity tyrosine regulated kinaseCyclin dependent kinaseβ-amyloid peptideAβAbetaTriazolothiadiazolesDYRK1ADual-specificity tyrosine phosphorylation-regulated kinase 1ACLK1CDC-like kinase 1Computer-aided drug designCADDInhibitorinhibitorinhibit

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