Art. -Nr.: HY-W004500

All-trans-retinal

3 Cited Publications

Kundenbewertung

Based on 3 publication(s) in Google Scholar

All-trans-retinal is an vitamin A metabolite in the retina, and is produced following photo-isomerization of the visual chromophore 11-cis-Retinal. All-trans-retinal is cleared from photoreceptors by ATP-binding cassette transporter (ABCA4) and all-trans-retinol dehydrogenase (RDH). All-trans-retinal induces Bax activation via DNA damage to mediate retinal cell apoptosis.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 99.56%
CAS. Nr.: 116-31-4
Formel: C₂₀H₂₈O
Molecular Weight:284.44

Speicherung:

-20°C, protect from light, stored under nitrogen

* The compound is unstable in solutions, freshly prepared is recommended.

All-trans-retinal Chemical Structure

CAS. Nr.: 116-31-4

Größe

Preis

Verfügbarkeit

Menge

Amount: USD 0.00

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (3)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Human Endogenous Metabolite

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

In Vitro

All-trans-retinal (10-30 μM; 16 h) displays a dose-dependent toxicity which included the morphological disruption of ARPE-19 cells and a decrease in their viability is observed to be time-dependent measured by LDH assay^[1].
All-trans-retinal (10-30 μM; 30 mim) increases the phosphorylation of p53 at Ser 46 and 8-OHdG production^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

ARPE-19 cells
Concentration:

10 μM, 20 μM, 30 μM
Incubation Time:

16 h
Result:

Displayed a dose-dependent toxicity which included the morphological disruption of ARPE-19 cells.

Western Blot Analysis^[1]

Cell Line:

ARPE-19 cells
Concentration:

10 μM, 30 μM
Incubation Time:

30 min
Result:

The phosphorylation of p53 at Ser 46 were detected prior to Bax activation in ARPE-19 cells.

Chemical Information

CAS. Nr. 116-31-4
Appearance Solid
Molecular Weight 284.44
Formel C₂₀H₂₈O
Color Light yellow to yellow
SMILES

CC(/C=C/C=C(/C=C/C1=C(CCCC(C)1C)C)C)=C\C=O
Structure Classification
- Terpenoids
- Diterpenoids
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, protect from light, stored under nitrogen

* The compound is unstable in solutions, freshly prepared is recommended.

Publications (3)

All Cell Proliferation/Viability AssayBio/Physico-chemical Assay

Sort by:

Journal Impact Factor

Cell Res

Light sensing enhances thermotolerance and competitive fitness via serotonergic signaling in an eyeless organism. [Abstract]

2026 Apr;36(4):286-299. PMID: 41764323
Nat Commun

Suppression of ferroptosis by vitamin A or radical-trapping antioxidants is essential for neuronal development. [Abstract]

2024 Sep 1;15(1):7611. PMID: 39218970

All-trans-retinal purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Sep 1;15(1):7611. [Abstract]

CellTiter-Glo assay of HT-1080 cells co-treated with RSL3 and ATRA, Retinol or Retinal (n = 3 biologically independent replicates) – All-trans-retinal (Retinal) and Retinol at concentrations > 3 µM were toxic.

All-trans-retinal purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Sep 1;15(1):7611. [Abstract]

C11-BODIPY cell-free assay of the radical initiator AAPH co-treated with ATRA, Retinol or All-trans-retinal (Retinal). The results showed that Retinal significantly reduced the C11-BODIPY signal induced by AAPH in the cell-free system, indicating its antioxidant activity in free radical scavenging.
J Biol Chem

A cell-based assay for retinaldehyde dehydrogenase activity: Retinoid quantification as an alternative to current fluorescence-based approaches. [Abstract]

2026 Jan 29;302(3):111211. PMID: 41617028

All-trans-retinal purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 29;302(3):111211. [Abstract]

mutuDC1940 cells were treated for 20 h with 1 μM 9cRA, then assayed for RALDH activity in the suspension cell forma, utilising the indicated All-trans-retinal (RAL, 2.5-10 μM) concentrations for 20 min.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (351.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.5157 mL

17.5784 mL

35.1568 mL

5 mM

0.7031 mL

3.5157 mL

7.0314 mL

10 mM

0.3516 mL

1.7578 mL

3.5157 mL

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (7.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.08 mg/mL (7.31 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (7.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (557 KB)
- English - EN (557 KB)
- Français - FR (557 KB)
- Deutsch - DE (557 KB)
- Norwegian - NO (557 KB)
- Español - ES (557 KB)
- Swedish - SV (557 KB)
- Italian - IT (557 KB)
- Korean - KR (557 KB)
- Portuguese - PT (557 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Sawada O, et al. All-trans-retinal induces Bax activation via DNA damage to mediate retinal cell apoptosis. Exp Eye Res. 2014 Jun;123:27-36. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.5157 mL	17.5784 mL	35.1568 mL	87.8920 mL
	5 mM	0.7031 mL	3.5157 mL	7.0314 mL	17.5784 mL
	10 mM	0.3516 mL	1.7578 mL	3.5157 mL	8.7892 mL
	15 mM	0.2344 mL	1.1719 mL	2.3438 mL	5.8595 mL
	20 mM	0.1758 mL	0.8789 mL	1.7578 mL	4.3946 mL
	25 mM	0.1406 mL	0.7031 mL	1.4063 mL	3.5157 mL
	30 mM	0.1172 mL	0.5859 mL	1.1719 mL	2.9297 mL
	40 mM	0.0879 mL	0.4395 mL	0.8789 mL	2.1973 mL
	50 mM	0.0703 mL	0.3516 mL	0.7031 mL	1.7578 mL
	60 mM	0.0586 mL	0.2930 mL	0.5859 mL	1.4649 mL
	80 mM	0.0439 mL	0.2197 mL	0.4395 mL	1.0986 mL
	100 mM	0.0352 mL	0.1758 mL	0.3516 mL	0.8789 mL

Keywords

All-trans-retinal

3 Cited Publications

100 mg Angebot einholen

Subscribe to the

MedChemExpress

Newsletter

Produktname:
Art. -Nr.:
Menge:
MCE Japan Authorized Agent:

Produktname:
CAS. Nr.:
Species:
Reinheit:
Menge:

All-trans-retinal

MCE Bioactive Compound Library

Natural Product Library Plus

Cosmetic Ingredient Compound Library

Publications Citing Use of MedChemExpress (MCE) All-trans-retinal

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (3)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

All-trans-retinal

MCE Bioactive Compound Library

Natural Product Library Plus

Cosmetic Ingredient Compound Library

Publications Citing Use of MedChemExpress (MCE) All-trans-retinal

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (3)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

All-trans-retinal Related Classifications

HY-W004500 Related Classifications