Art. -Nr.: HY-19528

SAH

(Synonyms: SAH (S-Adenosylhomocysteine))

20 Cited Publications

Kundenbewertung

Based on 20 publication(s) in Google Scholar

SAH (S-Adenosylhomocysteine) is an amino acid derivative and a modulartor in several metabolic pathways. It is an intermediate in the synthesis of cysteine and adenosine. SAH is an inhibitor for METTL3-METTL14 heterodimer complex (METTL3-14) with an IC₅₀ of 0.9 µM.

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Reinheit: 99.90%
CAS. Nr.: 979-92-0
Formel: C₁₄H₂₀N₆O₅S
Molecular Weight:384.41

Speicherung:

-20°C, protect from light

* In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

SAH Chemical Structure

CAS. Nr.: 979-92-0

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (20)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

SAH (S-Adenosylhomocysteine) is an amino acid derivative and a modulartor in several metabolic pathways. It is an intermediate in the synthesis of cysteine and adenosine^[1]. SAH is an inhibitor for METTL3-METTL14 heterodimer complex (METTL3-14) with an IC₅₀ of 0.9 µM^[2].

IC₅₀ & Target

Human Endogenous Metabolite

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	0.14 μM Compound: SAH	Inhibition of DOTL1 (unknown origin) using [3H]SAM and HeLa oligo nucleosomes as substrates incubated for 1 hr Inhibition of DOTL1 (unknown origin) using [3H]SAM and HeLa oligo nucleosomes as substrates incubated for 1 hr	[PMID: 35525212]
Sf9	IC₅₀	>50 μM Compound: SAH	Inhibition of human full length DNMT3A expressed in Sf9 cells Inhibition of human full length DNMT3A expressed in Sf9 cells	[PMID: 25893041]
Sf9	IC₅₀	>50 μM Compound: SAH	Inhibition of human full length DNMT3B expressed in Sf9 cells Inhibition of human full length DNMT3B expressed in Sf9 cells	[PMID: 25893041]
Sf9	IC₅₀	>50 μM Compound: SAH	Inhibition of human full length PRMT5 expressed in Sf9 cells Inhibition of human full length PRMT5 expressed in Sf9 cells	[PMID: 25893041]
Sf9	IC₅₀	>50 μM Compound: SAH	Inhibition of human full length PRMT7 expressed in Sf9 cells Inhibition of human full length PRMT7 expressed in Sf9 cells	[PMID: 25893041]
Sf9	IC₅₀	1.9 μM Compound: 1, SAH	Inhibition of N-terminally FLAG-tagged EZH2 Y641N mutant in EZH2/SUZ12/EED/RbAp48 complex (unknown origin) expressed in baculovirus infected in SF9 cells assessed as inhibition of methylation of nucleosomes at H3K27 by scintillation counting in presence o Inhibition of N-terminally FLAG-tagged EZH2 Y641N mutant in EZH2/SUZ12/EED/RbAp48 complex (unknown origin) expressed in baculovirus infected in SF9 cells assessed as inhibition of methylation of nucleosomes at H3K27 by scintillation counting in presence o	[PMID: 25746813]
Sf9	IC₅₀	11 μM Compound: 1, SAH	Inhibition of N-terminally FLAG-tagged wild type EZH2 in EZH2/SUZ12/EED/RbAp48 complex (unknown origin) expressed in baculovirus infected in SF9 cells assessed as inhibition of methylation of nucleosomes at H3K27 by scintillation counting in presence of [ Inhibition of N-terminally FLAG-tagged wild type EZH2 in EZH2/SUZ12/EED/RbAp48 complex (unknown origin) expressed in baculovirus infected in SF9 cells assessed as inhibition of methylation of nucleosomes at H3K27 by scintillation counting in presence of [	[PMID: 25746813]
Sf9	IC₅₀	4 μM Compound: SAH	Inhibition of human recombinant DNMT1 expressed in Sf9 cells assessed as incorporation of [3H]S-adenosyl methionine into hemimethylated oligonucleotide substrate after 3 hrs by scintillation counting Inhibition of human recombinant DNMT1 expressed in Sf9 cells assessed as incorporation of [3H]S-adenosyl methionine into hemimethylated oligonucleotide substrate after 3 hrs by scintillation counting	[PMID: 21958292]

In Vitro

SAH (10^-7-10^-5 M) activates norepinephrine (NE) and serotonin (5-HT) in vitro uptake in synaptosomal preparations from rat brain, but does not affect dopamine (DA) uptake^[1].
SAH (0.1-10 μM) shows strong inhibitory effects on METTL3-14 activity with an IC₅₀ value of 0.9 μM in Kinetic parameter determination of the METTL3-14 complex^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

SAH (administered i.v., i.p. or p.o. in 1-10 mg/kg) is sleep-inductive and anticonvulsant with rabbit, rat and cat^[1].
SAH (intraperitoneal injection; 7 mg/kg; 1 h prior to sacrifice) induces in vitro uptake increase for NE and 5-HT, significant only in brain stem and midbrain, but does not alter DA uptake^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 979-92-0
Appearance Solid
Molecular Weight 384.41
Formel C₁₄H₂₀N₆O₅S
Color White to off-white
SMILES

NC1=NC=NC2=C1N=CN2[C@@H]3O[C@H](CSCC[C@H](N)C(O)=O)[C@@H](O)[C@H]3O
Synonyms

SAH (S-Adenosylhomocysteine)
Structure Classification
- Amino acids
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, protect from light

* In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

Publications (20)

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Journal Impact Factor

Cell Res

The AHCY-adenosine complex rewires mRNA methylation to enhance fatty acid biosynthesis and tumorigenesis. [Abstract]

2026 Feb;36(2):152-172. PMID: 41549122
Adv Sci (Weinh)

Methionine Metabolism Dictates PCSK9 Expression and Antitumor Potency of PD-1 Blockade in MSS Colorectal Cancer. [Abstract]

2025 Mar 24:e2501623. PMID: 40125618

SAH purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Mar 24:e2501623. [Abstract]

Immunoblotting analysis of PCSK9 in SW480 (upper panel) and SW620 (lower panel) cells with methionine deprivation for 6 h and then SAM E) or SAH F) supplementation for 24h.
Redox Biol

Plin4 exacerbates cadmium-decreased testosterone level via inducing ferroptosis in testicular Leydig cells. [Abstract]

2024 Aug 17:76:103312. PMID: 39173539

SAH purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Aug 17:76:103312. [Abstract]

SAH (10 μM) intervention markedly alleviated environmental stress-induced elevation of PLIN4 levels and accumulation of lipid droplets.
J Hazard Mater

Heat stress enhances the expression of METTL3 to mediate N6-methyladenosine modification of SOS2 and NLRP3 inflammasome activation in boar Sertoli cells. [Abstract]

2025 Jan 28:488:137432. PMID: 39884044
Cancer Lett

The Actin Histidine methyltransferase SETD3 is a CHD1 lysine di-methyltransferase. [Abstract]

2025 Oct 2:218073. PMID: 41045985
Proc Natl Acad Sci U S A

A PHF19-YTHDC1 condensate switches EZH2-mediated gene suppression to activation for prostate cancer progression. [Abstract]

2025 Oct 28;122(43):e2510386122. PMID: 41129231
Dev Cell

CircRNA-encoded RIPK1-98 protein drives lung adenocarcinoma progression. [Abstract]

2026 Mar 12:S1534-5807(26)00079-1. PMID: 41825439
J Crohns Colitis

Intermittent ketogenic diet promotes Treg differentiation and alleviates Crohn's disease via the β-hydroxybutyrate driven AHCY-DNMT1-Foxp3 axis. [Abstract]

2026 Feb 5;20(2):jjag003. PMID: 41527294
Apoptosis

p53 m6A modulation sensitizes hepatocellular carcinoma to apatinib through apoptosis. [Abstract]

2022 Jun;27(5-6):426-440. PMID: 35503144
Mol Med

Epigenetic regulatory mechanism of ADAMTS12 expression in osteoarthritis. [Abstract]

2023 Jul 3;29(1):86. PMID: 37400752

SAH purchased from MedChemExpress. Usage Cited in: Mol Med. 2023 Jul 3;29(1):86. [Abstract]

The results of HE, PAS, and safranin O-fast green revealed that the cartilage damage and fibrosis induced by ACL-T were mitigated because of METTL3 inhibitor SAH (10 mg/kg).
Ecotoxicol Environ Saf

Bisphenol A disrupts circadian locomotor rhythms via m6A-dependent nr1d1 destabilization in zebrafish larvae. [Abstract]

2026 Mar 15:313:119887. PMID: 41797106
Commun Biol

METTL3 obstructs vascular smooth muscle cells osteogenic reprogramming by methylating Runx2 in chronic kidney disease. [Abstract]

2025 Apr 8;8(1):582. PMID: 40200050

SAH purchased from MedChemExpress. Usage Cited in: Commun Biol. 2025 Apr 8;8(1):582. [Abstract]

METTL3 was effectively decreased in the VSMCs of SAH (S-Adenosylhomocysteine, 5 mg/kg, twice a week) treated CKD rats, accompanied with the elevation of Runx2 at the same localization.
Int J Mol Sci

Melatonin Alleviates Lipopolysaccharide-Induced Abnormal Pregnancy through MTNR1B Regulation of m6A. [Abstract]

2024 Jan 5;25(2):733. PMID: 38255808
Eur J Pharmacol

Single-cell sequencing reveals that specnuezhenide protects against osteoporosis via activation of METTL3 in LEPR+ BMSCs. [Abstract]

2024 Aug 21:981:176908. PMID: 39154827
J Chromatogr A

Quantification of five intracellular and extracellular methionine pathway intermediates using stable isotope dilution UHPLC-MS/MS. [Abstract]

2025 May 10:1755:466036. PMID: 40398135
Chem Res Toxicol

RNA Methylation and Transcriptome Analysis Reveal Key Regulatory Pathways Related to Cadmium-Induced Liver Damage. [Abstract]

2025 Mar 26. PMID: 40135526
Cell Signal

METTL14-mediated m6A modification of TRPA1 promotes acute visceral pain induced by uterine cervical dilation by promoting NR2B phosphorylation. [Abstract]

2025 Jan 16:127:111610. PMID: 39826676
Bone

Mettl3 inhibits the apoptosis and autophagy of chondrocytes in inflammation through mediating Bcl2 stability via Ythdf1-mediated m6A modification. [Abstract]

2022 Jan;154:116182. PMID: 34530171
Oncol Lett

RNA m6A methyltransferase METTL3 promotes colorectal cancer cell proliferation and invasion by regulating Snail expression. [Abstract]

2021 Oct;22(4):711. PMID: 34457066
bioRxiv

2023 Apr 19.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (260.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6014 mL

13.0069 mL

26.0139 mL

5 mM

0.5203 mL

2.6014 mL

5.2028 mL

10 mM

0.2601 mL

1.3007 mL

2.6014 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. DE LA HABA G, et al. The enzymatic synthesis of S-adenosyl-L-homocysteine from adenosine and homocysteine. J Biol Chem. 1959 Mar;234(3):603-8. [Content Brief]

[2]. Li F, et al. A Radioactivity-Based Assay for Screening Human m6A-RNA Methyltransferase, METTL3-METTL14 Complex, and Demethylase ALKBH5. Biomol Screen. 2016 Mar;21(3):290-7. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6014 mL	13.0069 mL	26.0139 mL	65.0347 mL
	5 mM	0.5203 mL	2.6014 mL	5.2028 mL	13.0069 mL
	10 mM	0.2601 mL	1.3007 mL	2.6014 mL	6.5035 mL
	15 mM	0.1734 mL	0.8671 mL	1.7343 mL	4.3356 mL
	20 mM	0.1301 mL	0.6503 mL	1.3007 mL	3.2517 mL
	25 mM	0.1041 mL	0.5203 mL	1.0406 mL	2.6014 mL
	30 mM	0.0867 mL	0.4336 mL	0.8671 mL	2.1678 mL
	40 mM	0.0650 mL	0.3252 mL	0.6503 mL	1.6259 mL
	50 mM	0.0520 mL	0.2601 mL	0.5203 mL	1.3007 mL
	60 mM	0.0434 mL	0.2168 mL	0.4336 mL	1.0839 mL
	80 mM	0.0325 mL	0.1626 mL	0.3252 mL	0.8129 mL
	100 mM	0.0260 mL	0.1301 mL	0.2601 mL	0.6503 mL

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SAH

Metabolism/Protease Compound Library

Natural Product Library

Human Endogenous Metabolite Compound Library

Nucleotide Compound Library

Gut Microbial Metabolite Library

Microbial Metabolite Library

Food-Sourced Compound Library

Human Metabolite Library

Heterocyclic Compound Library

Animal-Sourced Natural Product Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Endogenous Metabolite Library

Mass Spectrometry Human Metabolite Library

Mouse Metabolite Compound Library

Amino Acid Metabolite Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) SAH

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (20)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

SAH

Metabolism/Protease Compound Library

Natural Product Library

Human Endogenous Metabolite Compound Library

Nucleotide Compound Library

Gut Microbial Metabolite Library

Microbial Metabolite Library

Food-Sourced Compound Library

Human Metabolite Library

Heterocyclic Compound Library

Animal-Sourced Natural Product Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Endogenous Metabolite Library

Mass Spectrometry Human Metabolite Library

Mouse Metabolite Compound Library

Amino Acid Metabolite Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) SAH

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (20)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

SAH Related Classifications

HY-19528 Related Classifications