Art. -Nr.: HY-13963

ZCL278

4 Cited Publications

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Based on 4 publication(s) in Google Scholar

ZCL278 is a selective Cdc42 inhibitor with K_ds of 6.4 μM in fluorescence titration and 11.4 μM in surface plasmon resonance (SPR) measurement. ZCL278 is able to disrupt the Cdc42-ITSN interaction as well as GTP/GDP binding. ZCL278 has inhibitory effects on various enveloped viruses, but is ineffective against non-enveloped viruses. ZCL278 can be used for the studies of arsenic neurotoxicity and HER2-positive gastric cancer (GC).

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Reinheit: 98.49%
CAS. Nr.: 587841-73-4
Formel: C₂₁H₁₉BrClN₅O₄S₂
Molecular Weight:584.89

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

ZCL278 Chemical Structure

CAS. Nr.: 587841-73-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Kd: 11.4 μM (Cdc42)^[1]

Alle Ras Isoform-spezifische Produkte anzeigen :

In Vitro

ZCL278 (5-50 μM, 24 h) impedes wound healing without disruption of cell viability in PC-3 cells^[1].
ZCL278 (50 μM, 5-15 min) exhibits a time-dependent inhibition of Cdc42 activity in PC-3 cells^[1].
ZCL278 (50 μM, 0-10 min) inhibits mice primary neurons branchingand growthconemotility^[1].
ZCL278 (0.01-1000 μM, 0-18 h) does not interfere with Junin virus (JUNV) attachment to the cell surface or virus particle internalization into host cells, it prevents the release of JUNV ribonucleoprotein cores into the cytosol and decreases pH-mediated viral fusion with host membranes in Vero, SUM159, SVG-A, or A549 cells^[2].
ZCL278 (50 μM, 6-24 h) significantly inhibits VSV, LCMV and DV2 infections, but has no effect on non-enveloped virus PV1 in Vero or Huh7 cells^[2].
ZCL278 (20 μM, 25 h) improves the cytotoxicity of NaAsO₂ in rat astrocytes^[3].
ZCL278 reduces cholesterol levels in the plasma membrane and reverses collateral resistance between Trastuzumab (HY-P9907) and chemotherapy in TC cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Migration Assay ^[1]

Cell Line:

PC-3 cells
Concentration:

5 and 50 μM
Incubation Time:

24 h
Result:

Dose-dependently inhibited cell migration (50 μM inhibits by 92%), did not affect cell viability, and eliminated the interference of toxicity.

Western Blot Analysis^[1]

Cell Line:

PC-3 cells
Concentration:

50 μM
Incubation Time:

5, 10, 15 min
Result:

Inhibited endogenous Rac/Cdc42 activities.
Inhibited Cdc42 activity.

In Vivo

ZCL278 (1-100 μg/kg, i.p., once daily for 4 days) inhibits JUNV replication in a mouse model, and no toxicity is detected^[2].
ZCL278 (50 mg/kg, i.p., every four days for 33 days) effectively reduces cholesterol enrichment in the plasma membrane and reverses collateral resistance between Trastuzumab and chemotherapy in ^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

JUNV infection model established in C57BL/6 mice ^[2]
Dosage:

1, 10 and 100 μg/kg
Administration:

Intraperitoneal injection (i.p.), once daily for 4 days
Result:

Reduced the viral load in the spleen by more than 33 times.
Did not affect the weight of mice.

Animal Model:

Trastuzumab-resistant model established in female nude mice aged 3–4 weeks^[4]
Dosage:

50 mg/kg
Administration:

Intraperitoneal injection (i.p.), every four days for 33 days
Result:

Remarkably suppressed tumor growth, while PTX/DTX alone failed to exert anti-tumor efficacy.
Markedly decreased Cdc42-GTP activity and mem brane cholesterol contents.

Chemical Information

CAS. Nr. 587841-73-4
Appearance Solid
Molecular Weight 584.89
Formel C₂₁H₁₉BrClN₅O₄S₂
Color White to light yellow
SMILES

O=C(NC(NC1=CC=C(S(=O)(NC2=NC(C)=CC(C)=N2)=O)C=C1)=S)COC3=CC=C(Br)C=C3Cl
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (4)

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Journal Impact Factor

Nature

Submicrometre sampling of living cells by macrophages. [Abstract]

2026 Jun;654(8118):495-503. PMID: 42056510
Nat Immunol

Migrating immune cells globally coordinate protrusive forces. [Abstract]

2025 Aug;26(8):1258-1266. PMID: 40664976
Cell Mol Immunol

Spatial distribution of IL4 controls iNKT cell-DC crosstalk in tumors. [Abstract]

2020 May;17(5):496-506. PMID: 31160756
FASEB J

L-lactate promotes intestinal epithelial cell migration to inhibit colitis. [Abstract]

2021 Apr;35(4):e21554. PMID: 33742715

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (85.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7097 mL

8.5486 mL

17.0972 mL

5 mM

0.3419 mL

1.7097 mL

3.4194 mL

10 mM

0.1710 mL

0.8549 mL

1.7097 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.27 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.27 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (597 KB)
- English - EN (597 KB)
- Français - FR (597 KB)
- Deutsch - DE (597 KB)
- Norwegian - NO (597 KB)
- Español - ES (597 KB)
- Swedish - SV (597 KB)
- Italian - IT (597 KB)
- Korean - KR (597 KB)
- Portuguese - PT (597 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Friesland A, et al. Small molecule targeting Cdc42-intersectin interaction disrupts Golgi organization and suppresses cell motility. Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1261-6. [Content Brief]

[2]. Chou YY, et al. Identification and Characterization of a Novel Broad-Spectrum Virus Entry Inhibitor. J Virol. 2016 Apr 14;90(9):4494-510. [Content Brief]

[3]. An Y, Liu T, Liu X, Zhao L, Wang J. Rac1 and Cdc42 Play Important Roles in Arsenic Neurotoxicity in Primary Cultured Rat Cerebellar Astrocytes. Biol Trace Elem Res. 2016 Mar;170(1):173-82. [Content Brief]

[4]. Liang B, et al. Cdc42-driven endosomal cholesterol transport promotes collateral resistance in HER2-positive gastric cancer. Cancer Lett. 2024 Apr 10;587:216702. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.7097 mL	8.5486 mL	17.0972 mL	42.7431 mL
	5 mM	0.3419 mL	1.7097 mL	3.4194 mL	8.5486 mL
	10 mM	0.1710 mL	0.8549 mL	1.7097 mL	4.2743 mL
	15 mM	0.1140 mL	0.5699 mL	1.1398 mL	2.8495 mL
	20 mM	0.0855 mL	0.4274 mL	0.8549 mL	2.1372 mL
	25 mM	0.0684 mL	0.3419 mL	0.6839 mL	1.7097 mL
	30 mM	0.0570 mL	0.2850 mL	0.5699 mL	1.4248 mL
	40 mM	0.0427 mL	0.2137 mL	0.4274 mL	1.0686 mL
	50 mM	0.0342 mL	0.1710 mL	0.3419 mL	0.8549 mL
	60 mM	0.0285 mL	0.1425 mL	0.2850 mL	0.7124 mL
	80 mM	0.0214 mL	0.1069 mL	0.2137 mL	0.5343 mL

ZCL278

4 Cited Publications

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ZCL278

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Anti-Colorectal Cancer Compound Library

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Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Anti-Hematopathy Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Protein Kinase Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) ZCL278

Alle Ras Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (4)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

ZCL278 Related Classifications

HY-13963 Related Classifications