Art. -Nr.: HY-N0282

Demecolcine

7 Cited Publications

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Based on 7 publication(s) in Google Scholar

Demecolcine is a potent mitotic inhibitor with an IC₅₀ value of 2.4 μM for inhibition of tubulin polymerization. Colcemid (Demecolcine) can interact with tubulin dimers to induce anti-mitotic action and inhibit microtubule growth. Colcemid (Demecolcine) can be used for inflammatory disorders and cancer research.

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Reinheit: 99.75%
CAS. Nr.: 477-30-5
Formel: C₂₁H₂₅NO₅
Molecular Weight:371.43

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Demecolcine Chemical Structure

CAS. Nr.: 477-30-5

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (7)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	EC₅₀	0.09 μM Compound: Demecolcine	Activation of rat PXR expressed in human HepG2 cells after 24 hrs by luciferase reporter gene based luminescent analysis Activation of rat PXR expressed in human HepG2 cells after 24 hrs by luciferase reporter gene based luminescent analysis	[PMID: 20966043]
HepG2	EC₅₀	12.6 μM Compound: Demecolcine	Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis	[PMID: 20966043]
KB	ED₅₀	<0.25 μg/mL Compound: Demicolcine	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 7130986]
MCF7	EC₅₀	0.6 μM Compound: 6	Cytotoxicity against human MCF7 after 3 days by SRB assay Cytotoxicity against human MCF7 after 3 days by SRB assay	[PMID: 15730238]
NCI-H460	EC₅₀	0.69 μM Compound: 6	Cytotoxicity against human H460 after 3 days by SRB assay Cytotoxicity against human H460 after 3 days by SRB assay	[PMID: 15730238]
SF-268	EC₅₀	6.2 μM Compound: 6	Cytotoxicity against human SF-268 after 3 days by SRB assay Cytotoxicity against human SF-268 after 3 days by SRB assay	[PMID: 15730238]

In Vitro

Demecolcine (0.1-0.25 μg/ml, 1 h) reduces the the hypoploidy frequencies in metaphase II complements of mouse, rat and frog spermatocytes^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Demecolcine (0.3 mg/kg for intraperitoneal injection) increases the incidence of metaphase II hypoploidy in demecolcine treated mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Mice^[3]
Dosage:

0.3 mg/kg
Administration:

Intraperitoneal injection (i.p.)
Result:

Elevated 7.8-8-fold of the hypoploid and hyperploid frequencies in metaphase II cells relative to controls.

Chemical Information

CAS. Nr. 477-30-5
Appearance Solid
Molecular Weight 371.43
Formel C₂₁H₂₅NO₅
Color White to light yellow
SMILES

O=C1C=C2[C@@H](NC)CCC3=CC(OC)=C(OC)C(OC)=C3C2=CC=C1OC
Structure Classification
- Alkaloids
- Other Alkaloids
Initial Source
- Plants
- other families
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (7)

All IP

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Journal Impact Factor

Adv Sci (Weinh)

ROS-Responsive Wedelolactone Hydrogel Promotes Intervertebral Disc Repair by Disrupting the NF-κB-LCN2 Inflammatory Feedback Loop. [Abstract]

2026 Mar;13(14):e21709. PMID: 41487054
Protein Cell

cGAS guards against chromosome end-to-end fusions during mitosis and facilitates replicative senescence. [Abstract]

2022 Jan;13(1):47-64. PMID: 34676498

Demecolcine purchased from MedChemExpress. Usage Cited in: Protein Cell. 2022 Jan;13(1):47-64. [Abstract]

ChIP analysis of TRF1, TRF2 and POT1 associating with telomeres in asynchronous (Asynchr) or mitotic U2OS cells. Cells were either asynchronous or synchronized at G1/S, released for 8 h and then treated with Colcemid (Demecolcine) for 12 h.

Demecolcine purchased from MedChemExpress. Usage Cited in: Protein Cell. 2022 Jan;13(1):47-64. [Abstract]

Co-IP assay to determine the domain of cGAS that interacts with CDK1. 293T cells transfected with GFP-tagged full-length (GFP-FL) or its domain (GFP-1, -2 or -3) were treated with Colcemid (Demecolcine: 1 μg/mL) for 16 h and subjected to immunoprecipitation with GFP-beads.
Clin Cancer Res

Amplification of extrachromosomal MYC paralogs shapes immunosuppressive tumor microenvironment in small cell lung cancer. [Abstract]

2025 Apr 29. PMID: 40299768
Cancer Lett

ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer. [Abstract]

2022 Oct 28:547:215855. PMID: 35998796
Acta Pharmacol Sin

PLN-L31A/I40A for the treatment of inherited heart disease caused by PLN-R14del mutations. [Abstract]

2026 Jan 16. PMID: 41545752
Cancer Cell Int

Machine learning identified extrachromosomal DNA-related 12 gene signatures to predict cancer immunotherapy response. [Abstract]

2025 Dec 18;25(1):436. PMID: 41413526
Oncol Lett

Diffuse large B‑cell lymphoma and monoclonal gammopathy secondary to immune thrombocytopenic purpura: A case report. [Abstract]

2023 Apr 19;25(6):237. PMID: 37153052

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 25 mg/mL (67.31 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 5 mg/mL (13.46 mM; ultrasonic and warming and heat to 60°C)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6923 mL

13.4615 mL

26.9230 mL

5 mM

0.5385 mL

2.6923 mL

5.3846 mL

10 mM

0.2692 mL

1.3461 mL

2.6923 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. T Tsuchida, et al. Colcemid-induced apoptosis of cultured human glioma: electron microscopic and confocal laser microscopic observation of cells sorted in different phases of cell cycle. Cytometry. 1998 Apr 1;31(4):295-9. [Content Brief]

[2]. Ashley M Rozario, et al. Ultra-Low Colcemid Doses Induce Microtubule Dysfunction as Revealed by Super-Resolution Microscopy. Bioexiv.

[3]. Nett RS, et al. Discovery and engineering of colchicine alkaloid biosynthesis. Nature. 2020 Aug;584(7819):148-153. doi: 10.1038/s41586-020-2546-8. Epub 2020 Jul 22. Erratum in: Nature. 2020 Jul 30. [Content Brief]

[4]. Muzaffar A, et al. Antitubulin effects of derivatives of 3-demethylthiocolchicine, methylthio ethers of natural colchicinoids, and thioketones derived from thiocolchicine. Comparison with colchicinoids. J Med Chem. 1990 Feb;33(2):567-71. [Content Brief]

[5]. Risley MS, et al. An improved method for cytogenetic analysis of meiotic aneuploidy in rodent and frog spermatocytes. Mutat Res. 1990 Dec;234(6):361-8. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	2.6923 mL	13.4615 mL	26.9230 mL	67.3074 mL
	5 mM	0.5385 mL	2.6923 mL	5.3846 mL	13.4615 mL
	10 mM	0.2692 mL	1.3461 mL	2.6923 mL	6.7307 mL
DMSO	15 mM	0.1795 mL	0.8974 mL	1.7949 mL	4.4872 mL
	20 mM	0.1346 mL	0.6731 mL	1.3461 mL	3.3654 mL
	25 mM	0.1077 mL	0.5385 mL	1.0769 mL	2.6923 mL
	30 mM	0.0897 mL	0.4487 mL	0.8974 mL	2.2436 mL
	40 mM	0.0673 mL	0.3365 mL	0.6731 mL	1.6827 mL
	50 mM	0.0538 mL	0.2692 mL	0.5385 mL	1.3461 mL
	60 mM	0.0449 mL	0.2244 mL	0.4487 mL	1.1218 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Demecolcine

7 Cited Publications

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Demecolcine

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Publications Citing Use of MedChemExpress (MCE) Demecolcine

Biologische Aktivität

Chemical Information

Publications (7)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Demecolcine Related Classifications

HY-N0282 Related Classifications