Art. -Nr.: HY-W001132

Indole

3 Cited Publications

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Based on 3 publication(s) in Google Scholar

Indole is an aromatic, heterocyclic, organic compound which widely distributed in the natural environment and can be produced by a variety of bacteria. Indole regulates various aspects of bacterial physiology, including spore formation, plasmid stability, resistance to drugs, biofilm formation, and virulence as an intercellular signal molecule.

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Reinheit: 99.99%
CAS. Nr.: 120-72-9
Formel: C₈H₇N
Molecular Weight:117.15

Speicherung:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

Indole Chemical Structure

CAS. Nr.: 120-72-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Clinical Trial
Chemical Information
Publications (3)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Microbial Metabolite

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>100 μM Compound: 2	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 22386528]
HEp-2	IC₅₀	>50 μM Compound: 24	Cytotoxicity against human Hep2 cells after 72 hrs by MTT assay Cytotoxicity against human Hep2 cells after 72 hrs by MTT assay	[PMID: 27116711]
HL-60	IC₅₀	>50 μM Compound: 24	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 27116711]
MDA-MB-231	IC₅₀	>100 μM Compound: 2	Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay	[PMID: 22386528]
MRC5	IC₅₀	15.58 μM Compound: 2	Cytotoxicity against human MRC5 cells after 48 hrs by MTT assay Cytotoxicity against human MRC5 cells after 48 hrs by MTT assay	[PMID: 22386528]
NCI-H292	IC₅₀	>50 μM Compound: 24	Cytotoxicity against human NCI-H292 cells after 72 hrs by MTT assay Cytotoxicity against human NCI-H292 cells after 72 hrs by MTT assay	[PMID: 27116711]
SK-N-SH	IC₅₀	1.78 μM Compound: 2	Cytotoxicity against human SK-N-SH cells after 48 hrs by MTT assay Cytotoxicity against human SK-N-SH cells after 48 hrs by MTT assay	[PMID: 22386528]
U-251	EC₅₀	>625 μM Compound: Indole	Antiproliferative activity against human U251MG cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human U251MG cells after 72 hrs by sulforhodamine B assay	[PMID: 30568753]
U-87MG ATCC	EC₅₀	>625 μM Compound: Indole	Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay	[PMID: 30568753]

In Vitro

Indole (2 μM, 48 h) inhibits cell proliferation and cell connection damage in HCT116 and SW620 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

HCT116, SW620
Concentration:

2 μM
Incubation Time:

48 h
Result:

Inhibited cell growth and moderated cell cycle progression from G1 to S phase in CRC cell lines HCT116 and SW620.

Western Blot Analysis^[2]

Cell Line:

HCT116, SW620
Concentration:

2 μM
Incubation Time:

48 h
Result:

Increased protein expressions of occludin and claudin-3.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 120-72-9
Appearance Solid
Molecular Weight 117.15
Formel C₈H₇N
Color Off-white to pale purple
SMILES

C1(C=CC=C2)=C2NC=C1
Structure Classification
- Alkaloids
- Indole Alkaloids
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Publications (3)

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Journal Impact Factor

Imeta

Black rice diet alleviates colorectal cancer development through modulating tryptophan metabolism and activating AHR pathway. [Abstract]

2024 Jan 15;3(1):e165. PMID: 38868519
Cell Metab

Gut microbial metabolism via hippocampal indole-AhR signaling regulates emotional symptoms. [Abstract]

2026 Apr 1:S1550-4131(26)00097-5. PMID: 41928504
University of North Carolina at Chapel Hill

2025.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (853.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 3.57 mg/mL (30.47 mM; ultrasonic and warming and heat to 60°C)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

8.5361 mL

42.6803 mL

85.3606 mL

5 mM

1.7072 mL

8.5361 mL

17.0721 mL

10 mM

0.8536 mL

4.2680 mL

8.5361 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.75 mg/mL (23.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.75 mg/mL (23.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.75 mg/mL (23.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (624 KB)
- English - EN (624 KB)
- Français - FR (624 KB)
- Deutsch - DE (624 KB)
- Norwegian - NO (624 KB)
- Español - ES (624 KB)
- Swedish - SV (624 KB)
- Italian - IT (624 KB)
- Korean - KR (624 KB)
- Portuguese - PT (624 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Lee JH, et al. Indole as an intercellular signal in microbial communities. FEMS Microbiol Rev. 2010 Jul;34(4):426-44. [Content Brief]

[2]. Wang L, et al. Black rice diet alleviates colorectal cancer development through modulating tryptophan metabolism and activating AHR pathway. Imeta. 2024 Jan 15;3(1):e165. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	8.5361 mL	42.6803 mL	85.3606 mL	213.4016 mL
	5 mM	1.7072 mL	8.5361 mL	17.0721 mL	42.6803 mL
	10 mM	0.8536 mL	4.2680 mL	8.5361 mL	21.3402 mL
	15 mM	0.5691 mL	2.8454 mL	5.6907 mL	14.2268 mL
	20 mM	0.4268 mL	2.1340 mL	4.2680 mL	10.6701 mL
	25 mM	0.3414 mL	1.7072 mL	3.4144 mL	8.5361 mL
	30 mM	0.2845 mL	1.4227 mL	2.8454 mL	7.1134 mL
DMSO	40 mM	0.2134 mL	1.0670 mL	2.1340 mL	5.3350 mL
	50 mM	0.1707 mL	0.8536 mL	1.7072 mL	4.2680 mL
	60 mM	0.1423 mL	0.7113 mL	1.4227 mL	3.5567 mL
	80 mM	0.1067 mL	0.5335 mL	1.0670 mL	2.6675 mL
	100 mM	0.0854 mL	0.4268 mL	0.8536 mL	2.1340 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Indole

3 Cited Publications

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Indole

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Publications Citing Use of MedChemExpress (MCE) Indole

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (3)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Indole Related Classifications

HY-W001132 Related Classifications