Neoruscogenin
Based on 1 publication(s) in Google Scholar
Neoruscogenin, a member of the steroidal sapogenin family, is a high-affinity agonist of the nuclear receptor RORα (NR1F1) (EC50 = 0.11 µM).
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- Reinheit : 99.21%
- CAS. Nr.: 17676-33-4
- Formel: C27H40O4
- Molecular Weight:428.60
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Speicherung:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Neoruscogenin
More
Biologische Aktivität
Beschreibung
In Vitro
Neoruscogenin (0.1-10 μM) increases the cell viability, but has no effect on cell viability at 50 μM, and decreases the cell viability at 100 μM[2].
Neoruscogenin (0.1 μM, 5 days) promotes C2C12 myoblast differentiation[2].
Neoruscogenin (0.1 μM, 5 days) activates Akt/mTOR/rpS6 signaling pathway in C2C12 myoblasts, and inhibits the Atrogin-1 and MuRF-1 mRNA levels[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:C2C12 myoblasts
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Concentration:0.1 μM
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Incubation Time:5 days
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Result:Increased protein expression of MyHC and MyOG at 0.1 μM, but did not work as well as 0.1 μM at 5 and 10 μM
In Vivo
Neoruscogenin (1 mg/kg,i.p. every 2 days for 8 times) induces skeletal muscle hypertrophy in mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:male ICR mice[2]
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Dosage:1 mg/kg
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Administration:i.p.
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Result:Showd the larger size of TA muscles, and increasaed the weight of Gas muscles.
Showed visible myofibers hypertrophy (H&E staining).
Chemical Information
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CAS. Nr. 17676-33-4
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Appearance Solid
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Molecular Weight 428.60
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Formel C27H40O4
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Color White to off-white
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SMILES
C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]3(OC4)CCC4=C)([H])C[C@@]1([H])[C@@](CC=C(C[C@@H](O)C5)[C@@]6([C@@H]5O)C)([H])[C@]6([H])CC2
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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J Biol Chem
PPARγ and C/EBPα enable adipocyte differentiation upon inhibition of histone methyltransferase PRC2 in malignant tumors. [Abstract]2024 Oct;300(10):107765. PMID: 39276936
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 50 mg/mL (116.66 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.56 mg/mL (1.31 mM); Clear solution
This protocol yields a clear solution of ≥ 0.56 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.6 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.56 mg/mL (1.31 mM); Clear solution
This protocol yields a clear solution of ≥ 0.56 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.6 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (277 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Helleboid S, et al. The identification of naturally occurring neoruscogenin as a bioavailable, potent, and high-affinity agonist of the nuclear receptor RORα (NR1F1).J Biomol Screen. 2014 Mar;19(3):399-406. [Content Brief]
[2]. Zhang D, et al. Novel Pro-myogenic Factor Neoruscogenin Induces Muscle Fiber Hypertrophy by Inhibiting MSTN Maturation and Activating the Akt/mTOR Pathway. J Agric Food Chem. 2023 Jan 11;71(1):499-511. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3332 mL | 11.6659 mL | 23.3318 mL | 58.3294 mL |
| 5 mM | 0.4666 mL | 2.3332 mL | 4.6664 mL | 11.6659 mL | |
| 10 mM | 0.2333 mL | 1.1666 mL | 2.3332 mL | 5.8329 mL | |
| 15 mM | 0.1555 mL | 0.7777 mL | 1.5555 mL | 3.8886 mL | |
| 20 mM | 0.1167 mL | 0.5833 mL | 1.1666 mL | 2.9165 mL | |
| 25 mM | 0.0933 mL | 0.4666 mL | 0.9333 mL | 2.3332 mL | |
| 30 mM | 0.0778 mL | 0.3889 mL | 0.7777 mL | 1.9443 mL | |
| 40 mM | 0.0583 mL | 0.2916 mL | 0.5833 mL | 1.4582 mL | |
| 50 mM | 0.0467 mL | 0.2333 mL | 0.4666 mL | 1.1666 mL | |
| 60 mM | 0.0389 mL | 0.1944 mL | 0.3889 mL | 0.9722 mL | |
| 80 mM | 0.0292 mL | 0.1458 mL | 0.2916 mL | 0.7291 mL | |
| 100 mM | 0.0233 mL | 0.1167 mL | 0.2333 mL | 0.5833 mL |
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