Art. -Nr.: HY-W013164

SC-58125

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SC-58125 is a potent and selective inhibitor of cyclooxygenase 2 (COX-2), with an IC₅₀ of 0.04 μM. SC-58125 exhibits antitumor activity in vitro and in vivo. SC-58125 also can inhibit edema at the inflammatory site and has analgesic effect.

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Reinheit: 99.92%
CAS. Nr.: 162054-19-5
Formel: C₁₇H₁₂F₄N₂O₂S
Molecular Weight:384.35

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

SC-58125 Chemical Structure

CAS. Nr.: 162054-19-5

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[1]

hCOX-2

0.04 μM (IC₅₀)

hCOX-1

>100 μM (IC₅₀)

Alle COX Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
Sf9	IC₅₀	>50 μM Compound: 25	Inhibitory activity against human recombinant Prostaglandin G/H synthase 1 expressed in microsomes taken from baculovirus infected Sf9 cells Inhibitory activity against human recombinant Prostaglandin G/H synthase 1 expressed in microsomes taken from baculovirus infected Sf9 cells	10.1016/0960-894X(95)00564-A
Sf9	IC₅₀	0.2 μM Compound: 25	Inhibitory activity against human recombinant Prostaglandin G/H synthase 2 expressed in microsomes taken from baculovirus infected Sf9 cells Inhibitory activity against human recombinant Prostaglandin G/H synthase 2 expressed in microsomes taken from baculovirus infected Sf9 cells	10.1016/0960-894X(95)00564-A
SW982	IC₅₀	33 nM Compound: SC-58125	Inhibition of PGE2 release from bradykinin-stimulated human SW982 cells by fluorescence assay Inhibition of PGE2 release from bradykinin-stimulated human SW982 cells by fluorescence assay	[PMID: 23581596]

In Vitro

SC-58125 (0.001-100 μM) has a high degree of selectivity for the inducible form of COX-2 (IC₅₀=1 μM) over the COX-1 (IC₅₀>100 μM)^[1].
SC-58125 (10 μM; 20-140 s) is time-dependent and is complete by 1 min, with a half-maximal inhibition at 20 s^[1].
SC-58125 (25-100 μM; 3 d) inhibits the in vitro growth of HCA-7 and LLC cells^[3].
SC-58125 (100 µM; 12 h) induces G₂ arrest in LLC cells^[3].
SC-58125 (25-100 μM; 3 d) decreases p34^cdc2 levels in HCA-7 cells^[3].
SC-58125 (100 µM; 24 or 72 h) does not induce apoptosis of HCA-7 and LLC cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[3]

Cell Line:

HCA-7 and LLC cells
Concentration:

0, 25, 50, 100 μM
Incubation Time:

3 days
Result:

Reduced the cell number and MTT activity in both cell lines in a dose-dependent manner.

Cell Cycle Analysis^[3]

Cell Line:

LLC cells
Concentration:

100 μM
Incubation Time:

12 hours
Result:

Increased in the number of cells containing 4n DNA content in a dose- and time-dependent manner.
Reduced the number of mitotic figures.

Western Blot Analysis^[3]

Cell Line:

HCA-7 cells
Concentration:

0, 25, 50, 100 μM
Incubation Time:

3 days
Result:

Resulted in a dose-dependent decrease in p34^cdc2 activity with strong inhibition, even at the lowest concentration.

In Vivo

SC-58125 (10 mg/kg; i.p. every 48 h) inhibits the growth of established colorectal cancer xenografts in mice^[3].
SC-58125 (10 mg/kg; a single i.p.) reduces tumor PGE2 levels in mice^[3].
SC-58125 (10 mg/kg; a single i.p.) does not change the tumor levels of COX-1 and COX-2 protein in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Athymic Sprague-Dawley mice are injected HCA-7 cells^[3]
Dosage:

10 mg/kg
Administration:

I.p. every 48 h; at the time of tumor implantation or 2 and 4 weeks later
Result:

Decreased the tumor growth rates significantly.

Chemical Information

CAS. Nr. 162054-19-5
Appearance Solid
Molecular Weight 384.35
Formel C₁₇H₁₂F₄N₂O₂S
Color Off-white to pink
SMILES

FC(C1=NN(C2=CC=C(S(=O)(C)=O)C=C2)C(C3=CC=C(F)C=C3)=C1)(F)F
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 250 mg/mL (650.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6018 mL

13.0090 mL

26.0180 mL

5 mM

0.5204 mL

2.6018 mL

5.2036 mL

10 mM

0.2602 mL

1.3009 mL

2.6018 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Gierse JK, et, al. A single amino acid difference between cyclooxygenase-1 (COX-1) and -2 (COX-2) reverses the selectivity of COX-2 specific inhibitors. J Biol Chem. 1996 Jun 28; 271(26): 15810-4. [Content Brief]

[2]. Seibert K, et, al. Pharmacological and biochemical demonstration of the role of cyclooxygenase 2 in inflammation and pain. Proc Natl Acad Sci U S A. 1994 Dec 6; 91(25): 12013-7. [Content Brief]

[3]. Williams CS, et, al. A cyclooxygenase-2 inhibitor (SC-58125) blocks growth of established human colon cancer xenografts. Neoplasia. Sep-Oct 2001; 3(5): 428-36. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6018 mL	13.0090 mL	26.0180 mL	65.0449 mL
	5 mM	0.5204 mL	2.6018 mL	5.2036 mL	13.0090 mL
	10 mM	0.2602 mL	1.3009 mL	2.6018 mL	6.5045 mL
	15 mM	0.1735 mL	0.8673 mL	1.7345 mL	4.3363 mL
	20 mM	0.1301 mL	0.6504 mL	1.3009 mL	3.2522 mL
	25 mM	0.1041 mL	0.5204 mL	1.0407 mL	2.6018 mL
	30 mM	0.0867 mL	0.4336 mL	0.8673 mL	2.1682 mL
	40 mM	0.0650 mL	0.3252 mL	0.6504 mL	1.6261 mL
	50 mM	0.0520 mL	0.2602 mL	0.5204 mL	1.3009 mL
	60 mM	0.0434 mL	0.2168 mL	0.4336 mL	1.0841 mL
	80 mM	0.0325 mL	0.1626 mL	0.3252 mL	0.8131 mL
	100 mM	0.0260 mL	0.1301 mL	0.2602 mL	0.6504 mL

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SC-58125

Immunology/Inflammation Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Pyroptosis Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Antidepressant Compound Library

Human Metabolite Library

Anti-Prostate Cancer Compound Library

Heterocyclic Compound Library

Highly Selective Inhibitors Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Anti-Rheumatic Arthritis Compound Library

Mass Spectrometry Human Metabolite Library

Alle COX Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

SC-58125

Immunology/Inflammation Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Pyroptosis Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Antidepressant Compound Library

Human Metabolite Library

Anti-Prostate Cancer Compound Library

Heterocyclic Compound Library

Highly Selective Inhibitors Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Anti-Rheumatic Arthritis Compound Library

Mass Spectrometry Human Metabolite Library

Alle COX Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

SC-58125 Related Classifications

HY-W013164 Related Classifications