ATG7-IN-3
Based on 1 Customer Validation
ATG7-IN-3 (Compound 18) is a potent ATG7 inhibitor with an IC50 of 0.048 μM. ATG7-IN-3 inhibits Autophagy. ATG7-IN-3 inhibits the formation of LC3B puncta. ATG7-IN-3 can be used in the researches of glioma and colon cancer.
For research use only. We do not sell to patients.
- Purity: 98.17%
- CAS No.: 2226229-76-9
- Formula: C11H16N6O5S2
- Molecular Weight:376.41
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
IC50: 0.048 ± 0.022 μM (ATG7), 0.280 ± 0.051 μM (Thioester), 0.938 ± 0.231 μM (LC3B)[1]
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| NCI-H1650 | EC50 |
10.4 μM
Compound: 18
|
Antiproliferative activity against human H1650 cells assessed as reduction in cell viability after 72 hrs by cell titer glow assay
Antiproliferative activity against human H1650 cells assessed as reduction in cell viability after 72 hrs by cell titer glow assay
|
[PMID: 32912429] |
| SK-OV-3 | EC50 |
>20 μM
Compound: 18
|
Induction of autophagy in human SKOV3 cells assessed as accumulation of NRB1 by immunofluorescence assay
Induction of autophagy in human SKOV3 cells assessed as accumulation of NRB1 by immunofluorescence assay
|
[PMID: 32912429] |
| SK-OV-3 | EC50 |
3 μM
Compound: 18
|
Induction of autophagy in human SKOV3 cells assessed as accumulation of p62 by immunofluorescence assay
Induction of autophagy in human SKOV3 cells assessed as accumulation of p62 by immunofluorescence assay
|
[PMID: 32912429] |
ATG7-IN-3 inhibits ATG7 with an IC50 value of 0.048 μM[1].
ATG7-IN-3 inhibits endogenous LC3B puncta formation in H4 cells (glioma cells) with an IC50 of 0.938 μM[1].
ATG7-IN-3 induces p62 accumulation in SKOV-3 cells (p62 EC50 of 3.0 μM)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 2226229-76-9
-
Appearance Solid
-
Molecular Weight 376.41
-
Formula C11H16N6O5S2
-
Color White to off-white
-
SMILES
CSC1=NN([C@]2([H])O[C@@H]([C@H](C2)O)COS(N)(=O)=O)C3=NC=NC(N)=C31
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (265.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.64 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.64 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (276 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6567 mL | 13.2834 mL | 26.5668 mL | 66.4169 mL |
| 5 mM | 0.5313 mL | 2.6567 mL | 5.3134 mL | 13.2834 mL | |
| 10 mM | 0.2657 mL | 1.3283 mL | 2.6567 mL | 6.6417 mL | |
| 15 mM | 0.1771 mL | 0.8856 mL | 1.7711 mL | 4.4278 mL | |
| 20 mM | 0.1328 mL | 0.6642 mL | 1.3283 mL | 3.3208 mL | |
| 25 mM | 0.1063 mL | 0.5313 mL | 1.0627 mL | 2.6567 mL | |
| 30 mM | 0.0886 mL | 0.4428 mL | 0.8856 mL | 2.2139 mL | |
| 40 mM | 0.0664 mL | 0.3321 mL | 0.6642 mL | 1.6604 mL | |
| 50 mM | 0.0531 mL | 0.2657 mL | 0.5313 mL | 1.3283 mL | |
| 60 mM | 0.0443 mL | 0.2214 mL | 0.4428 mL | 1.1069 mL | |
| 80 mM | 0.0332 mL | 0.1660 mL | 0.3321 mL | 0.8302 mL | |
| 100 mM | 0.0266 mL | 0.1328 mL | 0.2657 mL | 0.6642 mL |
ATG7-IN-3 Related Classifications
HY-146131 Related Classifications
Powered by Bioz