ATC12
Based on 1 Customer Validation
ATC12 is a Aurora-A kinase inhibitor. ATC12 binds to Aurora-A and competes with TPX2 for binding to disrupt the Aurora-A/TPX2 interaction. ATC12 inhibits cancer cell proliferation, induces apoptosis and cellular senescence. ATC12 can be used in the research of breast cancer.
For research use only. We do not sell to patients.
- Purity : 99.26%
- CAS No.: 384860-40-6
- Formula: C28H29NO3
- Molecular Weight:427.53
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All Aurora Kinase Isoforms
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Biological Activity
Description
In Vitro
ATC12 (1.56-100 μM; 10 min) disrupts the interaction between purified Aurora-AKDCM and the TPX21-43 peptide in vitro, with an IC50 of 25.6 μM[1].
ATC12 (10 μM; 24 h) reduces the formation of the Aurora-A/TPX2 complex on the mitotic spindle and the level of pT288 Aurora-A at spindle poles in U2OS osteosarcoma cells[1].
ATC12 (10 μM; 24 h) disrupts the formation of Aurora-A/TPX2 complexes in mitotic and interphase nuclei of MCF7 and MDA-MB-231 breast cancer cells, and reduces the nuclear level of Aurora-A[1].
ATC12 (30-50 μM; 0-48 h) inhibits the proliferation of MCF7 breast cancer cells, prolongs the mitotic process, reduces the cell growth rate, and induces apoptotic cell death[1].
ATC12 (30-50 μM; 0-72 h) inhibits the proliferation of MDA-MB-231 breast cancer cells, prolongs mitotic duration, reduces cell growth rate and induces cellular senescence without triggering cell death[1].
ATC12 (10-100 μM; 5 days) reduces the viability of patient-derived organoids BCO-21, BCO-46 and BCO-61 from triple-negative breast cancer (TNBC) in a dose-dependent manner[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:breast cancer MCF7 and MDA-MB-231 cells
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Concentration:10, 30, 50 μM
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Incubation Time:72 h
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Result:Decreased MCF7 cell viability in a dose-dependent manner, with significant reductions at 30 μM and 50 μM.
Caused a small but significant decrease in MDA-MB-231 cell viability only at 50 μM.
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Breast Cancer Modeling
- Senescence-associated β-galactosidase staining
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- Apoptosis Solutions
- MTT Cell Proliferation Assay
Chemical Information
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CAS No. 384860-40-6
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Appearance Solid
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Molecular Weight 427.53
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Formula C28H29NO3
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SMILES
CC(C)(C1=CC(C2=CC(C(O)=O)=C3C4=CC=CC=C4C=CC3=N2)=CC(C(C)(C)C)=C1O)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (116.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3390 mL | 11.6951 mL | 23.3902 mL | 58.4754 mL |
| 5 mM | 0.4678 mL | 2.3390 mL | 4.6780 mL | 11.6951 mL | |
| 10 mM | 0.2339 mL | 1.1695 mL | 2.3390 mL | 5.8475 mL | |
| 15 mM | 0.1559 mL | 0.7797 mL | 1.5593 mL | 3.8984 mL | |
| 20 mM | 0.1170 mL | 0.5848 mL | 1.1695 mL | 2.9238 mL | |
| 25 mM | 0.0936 mL | 0.4678 mL | 0.9356 mL | 2.3390 mL | |
| 30 mM | 0.0780 mL | 0.3898 mL | 0.7797 mL | 1.9492 mL | |
| 40 mM | 0.0585 mL | 0.2924 mL | 0.5848 mL | 1.4619 mL | |
| 50 mM | 0.0468 mL | 0.2339 mL | 0.4678 mL | 1.1695 mL | |
| 60 mM | 0.0390 mL | 0.1949 mL | 0.3898 mL | 0.9746 mL | |
| 80 mM | 0.0292 mL | 0.1462 mL | 0.2924 mL | 0.7309 mL | |
| 100 mM | 0.0234 mL | 0.1170 mL | 0.2339 mL | 0.5848 mL |
ATC12 Related Classifications
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