Cefotiam
Cefotiam (SCE-963) is a parenteral cephalosporin antibiotic. Cefotiam has broad-spectrum activity against Gram-positive and Gram-negative bacteria.
For research use only. We do not sell to patients.
- CAS No.: 61622-34-2
- Formula: C18H23N9O4S3
- Molecular Weight:525.63
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
IC50 & Target
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β-lactam |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| Oocyte | IC50 |
718 μM
Compound: Cefotiam
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TP_TRANSPORTER: inhibition of PAH uptake in Xenopus laevis oocytes
TP_TRANSPORTER: inhibition of PAH uptake in Xenopus laevis oocytes
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[PMID: 15618660] |
In Vitro
Cefotiam (SCE-963; 0-6.25μg/mL; 8 h) has antibacterial activity with a MIC value of 1.56 μg/mL for P. mirabilis IFO 3849[1].
Cefotiam (0-6.25μg/mL; 8 h) has highly active against both Staph. aureus (27 strains, MIC values of 0.5-1 μg/mL) and Staph. albus (8 strains, MICs 0.25-0.5 μg/mL). All 29 strains of haemolytic streptococci, 9 strains of pneumococci and 6 strains of Streptococcus viridans with the MIC values of in the range 0.06-4 μg/mL[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female CF1/b mice with P. mirabilis xenograft[1]
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Dosage:12.5, 25, 50, 100, 200, 400 and 800 mg/kg
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Administration:Subcutaneous injection; twice a day, for 5 days
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Result:Reduced or complete eradicated bacteria in the bladder walls and kidneys of mice sacrificed the day after treatment was terminated.
Chemical Information
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CAS No. 61622-34-2
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Molecular Weight 525.63
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Formula C18H23N9O4S3
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SMILES
O=C(C(N12)=C(CSC3=NN=NN3CCN(C)C)CS[C@]2([H])[C@H](NC(CC4=CSC(N)=N4)=O)C1=O)O
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Synonyms
SCE-963; Cefotiam Dihydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
Purity & Documentation
References
[1]. Iwahi T, et, al. Comparative activities of cefotiam and cefazolin against urinary tract infections with Proteus mirabilis in mice. Antimicrob Agents Chemother. 1980 Aug;18(2):257-63. [Content Brief]
[2]. Watt B, et, al. In-vitro activity of cefotiam against bacteria of clinical interest. J Antimicrob Chemother. 1982 Nov;10(5):391-5. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Cefotiam Related Classifications
HY-B0734 Related Classifications
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