Bafisontamab

[(G1 VK-CK-VH-CH1-h-CH2-CH3_L-kappa)_VH-CH1]-dimer

Immobilized Bafisontamab can bind Human EGFR Protein (ECD, His Tag). The EC₅₀for this effect is 3.76 ng/mL.

Immobilized Bafisontamab can bind Human c-MET/HGFR Protein (ECD, His Tag). The EC₅₀ for this effect is 3.28 ng/mL.

Flow Cytometry analysis of Hela cells labelling EGFR (red) with Bafisontamab (HY-P99455). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Flow cytometric analysis of 1X10⁶ HT-29 cells labeling MET with Bafisontamab (HY-P99455, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 for an hour at 4℃. AF488-conjugated Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa Isotype Control (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).