Buthiobate
Buthiobate (S-1358) is a fungicide. Buthiobate is a cytochrome P-450 inhibitor with an IC50 of 0.4 μM. Buthiobate binds to the heme iron of cytochrome P-450 (low-spin state) via its pyridine group, thereby blocking the initial hydroxylation of the 14α-methyl group. Buthiobate is used in research on powdery mildew in agricultural and horticultural crops.
For research use only. We do not sell to patients.
- CAS No.: 51308-54-4
- Formula: C21H28N2S2
- Molecular Weight:372.59
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Buthiobate (0.4-10 μM; 30 min) potently inhibits lanosterol 14α-demethylase activity in rat liver microsomes with an IC50 of 0.4 μM, and achieves near-complete inhibition at 10 μM[1].
Buthiobate (10-6-10-4 M; 3 h) potently inhibits C-14 demethylation in ergosterol biosynthesis in cell-free homogenates of Saccharomyces cerevisiae, with the strongest effect observed at 10-4 M, resulting in accumulation of lanosterol and reduced formation of downstream sterols[2].
Buthiobate (various concentrations; 36 μM) binds to three distinct low-spin cytochrome P-450 species in rat liver microsomes with KD values ranging from 0.38 to 13.2 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 51308-54-4
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Molecular Weight 372.59
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Formula C21H28N2S2
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SMILES
CCCCS/C(SCC1=CC=C(C=C1)C(C)(C)C)=N\C2=CC=CN=C2
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Synonyms
S-1358
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Buthiobate Related Classifications
HY-118558 Related Classifications
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