MST-312
Based on 2 publication(s) in Google Scholar
MST-312 is a telomerase inhibitor. MST-312 is a chemically modified derivative of green tea epigallocatechin gallate (EGCG). MST-312 can be used for the research of cancer, such as multiple myeloma (MM).
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté: 98.62%
- CAS No.: 368449-04-1
- Formule: C20H16N2O6
- Masse moléculaire:380.35
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) MST-312
More
Activité biologique
telomeras[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa | IC50 |
12.1 μM
Compound: 5, MST-312
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Inhibition of telomerase in human HeLa cells using 5'-AAT CCG TCG AGC AGA GTT-3' as substrate incubated for 15 mins prior to extension reaction by telomeric repeat amplification protocol
Inhibition of telomerase in human HeLa cells using 5'-AAT CCG TCG AGC AGA GTT-3' as substrate incubated for 15 mins prior to extension reaction by telomeric repeat amplification protocol
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[PMID: 22413845] |
| HeLa | IC50 |
12.8 μM
Compound: 5, MST-312
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Inhibition of telomerase in human HeLa cells using 5'-AAT CCG TCG AGC AGA GTT-3' as substrate incubated for 15 mins prior to extension reaction followed by compound washout by spin-telomeric repeat amplification protocol
Inhibition of telomerase in human HeLa cells using 5'-AAT CCG TCG AGC AGA GTT-3' as substrate incubated for 15 mins prior to extension reaction followed by compound washout by spin-telomeric repeat amplification protocol
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[PMID: 22413845] |
| U-937 | IC50 |
0.67 μM
Compound: 5, MST-312
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Inhibition of telomerase in human U937 cells by telomeric repeat amplification protocol
Inhibition of telomerase in human U937 cells by telomeric repeat amplification protocol
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[PMID: 22413845] |
| U-937 | IC50 |
0.67 μM
Compound: MST-312
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Inhibition of human telomerase isolated from human U937 cellular extracts by SYBR Green staining-based TRAP assay
Inhibition of human telomerase isolated from human U937 cellular extracts by SYBR Green staining-based TRAP assay
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[PMID: 24053596] |
| U-937 | IC50 |
0.67 μM
Compound: MST-312
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Inhibition of telomerase extracted from human U937 cells by TRAP assay
Inhibition of telomerase extracted from human U937 cells by TRAP assay
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10.1039/C0MD00241K |
| U-937 | IC50 |
0.67 μM
Compound: 24
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Antiproliferative activity against human U-937 cells measured upto 72 hrs
Antiproliferative activity against human U-937 cells measured upto 72 hrs
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[PMID: 37776575] |
| U-937 | IC50 |
1.7 μM
Compound: 24
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Antiproliferative activity against human U-937 cells assessed as cell growth inhibition measured after 72 hrs
Antiproliferative activity against human U-937 cells assessed as cell growth inhibition measured after 72 hrs
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[PMID: 37776575] |
MST-312 (2~8 μM; 0~72 hours; U-266 cells) reduces cellular viability in a dose dependent and time-dependent manner[1].
MST-312 (2~8 μM; 48 hours; U-266 cells) induces cell apoptosis in a dose-dependent manner[1].
MST-312 (2 μM; 48 hours; U-266 cells) up-regulates the pro-apoptotic gene Bax and down-regulates the anti-apoptotic gene Bcl-2 and suppresses the expression of c-Myc and hTERT genes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:U-266 cells
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Concentration:2~8 μM
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Incubation Time:0~72 hours
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Result:The viability of U-266 cells was substantially decreased in a dose dependent and time-dependent manner, in response to exposure to MST-312.
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Cell Line:U-266 cells
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Concentration:2~8 μM
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Incubation Time:48 hours
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Result:Induced cell apoptosis in a dose-dependent manner.
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Cell Line:U-266 cells
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Concentration:2 μM
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Incubation Time:48 hours
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Result:Up-regulated the pro-apoptotic gene Bax and down-regulated the anti-apoptotic gene Bcl-2 and suppressed the expression of c-Myc and hTERT genes.
Chemical Information
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CAS No. 368449-04-1
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Appearance Solid
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Masse moléculaire 380.35
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Formule C20H16N2O6
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Color White to off-white
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SMILES
O=C(NC1=CC=CC(NC(C2=CC=CC(O)=C2O)=O)=C1)C3=CC=CC(O)=C3O
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Synonyms
Telomerase Inhibitor IX
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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J Exp Med
Inhibition of hTERT/telomerase/telomere mediates therapeutic efficacy of osimertinib in EGFR mutant lung cancer. [Abstract]2024 Nov 4;221(11):e20240435. PMID: 39297884 -
Biomed Pharmacother
Azvudine exhibits potent differentiation-inducing effect by targeting the TERT/p21 axis in acute myeloid leukemia. [Abstract]2025 May 27:188:118215. PMID: 40435838
Solvant et solubilité
DMSO : 100 mg/mL (262.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.57 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.57 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (274 KB)
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SDS (458 KB)
- English - EN (458 KB)
- Français - FR (458 KB)
- Deutsch - DE (458 KB)
- Norwegian - NO (458 KB)
- Español - ES (458 KB)
- Swedish - SV (458 KB)
- Italian - IT (458 KB)
- Korean - KR (458 KB)
- Portuguese - PT (458 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6292 mL | 13.1458 mL | 26.2916 mL | 65.7289 mL |
| 5 mM | 0.5258 mL | 2.6292 mL | 5.2583 mL | 13.1458 mL | |
| 10 mM | 0.2629 mL | 1.3146 mL | 2.6292 mL | 6.5729 mL | |
| 15 mM | 0.1753 mL | 0.8764 mL | 1.7528 mL | 4.3819 mL | |
| 20 mM | 0.1315 mL | 0.6573 mL | 1.3146 mL | 3.2864 mL | |
| 25 mM | 0.1052 mL | 0.5258 mL | 1.0517 mL | 2.6292 mL | |
| 30 mM | 0.0876 mL | 0.4382 mL | 0.8764 mL | 2.1910 mL | |
| 40 mM | 0.0657 mL | 0.3286 mL | 0.6573 mL | 1.6432 mL | |
| 50 mM | 0.0526 mL | 0.2629 mL | 0.5258 mL | 1.3146 mL | |
| 60 mM | 0.0438 mL | 0.2191 mL | 0.4382 mL | 1.0955 mL | |
| 80 mM | 0.0329 mL | 0.1643 mL | 0.3286 mL | 0.8216 mL | |
| 100 mM | 0.0263 mL | 0.1315 mL | 0.2629 mL | 0.6573 mL |
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