Protosappanin B
Based on 2 publication(s) in Google Scholar
Protosappanin B is a phenolic compound extracted from Caesalpinia sappan. Anti-cancer activity. Protosappanin B induces apoptosis and causes G1 cell cycle arrest in human bladder cancer cells.
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- Pureté: 99.65%
- CAS No.: 102036-29-3
- Formule: C16H16O6
- Masse moléculaire:304.29
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Stockage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Publications Citing Use of MedChemExpress (MCE) Protosappanin B
More
Activité biologique
Apoptosis[2]
Protosappanin B (12.5, 25, 50, 100, 200 μg/mL, 48 hours) dose-dependently inhibits tumor cells, with IC50s of 21.32 μg/mL, 26.73 μg/mL, and 76.53 μg/mL for SW-480, HCT-116, and BTT cells, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:CT-116, SW-480, and BTT Cells
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Concentration:12.5, 25, 50, 100, 200 µg/mL
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Incubation Time:48 hours
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Result:Dose-dependently inhibited tumor cells after 48 hours of treatment.
Chemical Information
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CAS No. 102036-29-3
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Appearance Solid
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Masse moléculaire 304.29
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Formule C16H16O6
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Color Off-white to yellow
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SMILES
OC1=CC=C2C(OC[C@@](O)(CO)CC3=CC(O)=C(O)C=C23)=C1
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Synonyms
(-)-Protosappanin B
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (2)
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Journal Impact Factor
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Most Recent
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J Hematol Oncol
Allosteric activation of the metabolic enzyme GPD1 inhibits bladder cancer growth via the lysoPC-PAFR-TRPV2 axis. [Abstract]2022 Jul 14;15(1):93. PMID: 35836291 -
Free Radic Biol Med
Brazilin and its oxidized form "brazilein", the natural fluorescently traceable agents, toxify Plasmodium falciparum to pyknotic death via disrupting its iron homeostasis. [Abstract]2026 Feb 28:248:367-385. PMID: 41771360
Solvant et solubilité
DMSO : 50 mg/mL (164.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.84 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.84 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (279 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Yang X, et al. Antitumor Effects of Purified Protosappanin B Extracted From Lignum Sappan. Integr Cancer Ther. 2016 Mar;15(1):87-95. [Content Brief]
[2]. Yang X, et al. Protosappanin B promotes apoptosis and causes G1 cell cycle arrest in human bladder cancer cells. Sci Rep. 2019 Jan 31;9(1):1048. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2863 mL | 16.4317 mL | 32.8634 mL | 82.1585 mL |
| 5 mM | 0.6573 mL | 3.2863 mL | 6.5727 mL | 16.4317 mL | |
| 10 mM | 0.3286 mL | 1.6432 mL | 3.2863 mL | 8.2158 mL | |
| 15 mM | 0.2191 mL | 1.0954 mL | 2.1909 mL | 5.4772 mL | |
| 20 mM | 0.1643 mL | 0.8216 mL | 1.6432 mL | 4.1079 mL | |
| 25 mM | 0.1315 mL | 0.6573 mL | 1.3145 mL | 3.2863 mL | |
| 30 mM | 0.1095 mL | 0.5477 mL | 1.0954 mL | 2.7386 mL | |
| 40 mM | 0.0822 mL | 0.4108 mL | 0.8216 mL | 2.0540 mL | |
| 50 mM | 0.0657 mL | 0.3286 mL | 0.6573 mL | 1.6432 mL | |
| 60 mM | 0.0548 mL | 0.2739 mL | 0.5477 mL | 1.3693 mL | |
| 80 mM | 0.0411 mL | 0.2054 mL | 0.4108 mL | 1.0270 mL | |
| 100 mM | 0.0329 mL | 0.1643 mL | 0.3286 mL | 0.8216 mL |
Protosappanin B Related Classifications
HY-N0800 Related Classifications
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