CAN10 (WT IgG1)
Based on 1 Customer Validation
CAN10 (WT IgG1) is a monoclonal antibody targeting IL1RAP that retains the native, wild-type IgG1 Fc structure and has not undergone any engineered modifications. Unlike CAN10 (HY-P991267A), CAN10 (WT IgG1) does not contain the L234A and L235A mutations. CAN10 can be used in research concerning immune system disorders (such as systemic sclerosis).
For research use only. We do not sell to patients.
- Purity : 99.50%
- Molecular Weight:143.80 kDa
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Storage:Shipping with dry ice
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
Isotype
Human IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Human
IC50 & Target
IL1R3
In Vitro
CAN10 (WT IgG1) retains its native, wild-type IgG1 Fc structure without any engineered modifications. In contrast, the Fc region of CAN10 (HY-P991267A) incorporates L234A and L235A mutations, which are intended to reduce the antibody's binding to FcγR and C1q, thereby attenuating or eliminating its ADCC (antibody-dependent cell-mediated cytotoxicity) and CDC (complement-dependent cytotoxicity) effector functions.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Application
ELISA, FACS, Functional assay
Verified Bioactivity
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Flow cytometric analysis of 1X106 HepG2 cells labeling IL1R3 with CAN10 (HY-P991267, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 for an hour at 4℃. AF488-conjugated Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa Isotype Control (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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Appearance Liquid
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Molecular Weight 143.80 kDa
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Color Colorless to light yellow
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SMILES
[CAN10 (WT IgG1)]
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)