2. Anti-infection MAPK/ERK Pathway Stem Cell/Wnt GPCR/G Protein
  3. Antibiotic Raf ERK Ras MEK Bacterial
  4. Cefotetan

セフォテタン  (Synonyms: Cefotetan)

製品番号: HY-N6670 純度: 98.52%
COA 取扱説明書 Technical Support

Cefotetan is a binding agent that targets human Raf1 kinase inhibitor protein (hRKIP). Cefotetan binds to hRKIP, reduces the binding space between hRKIP and Raf1 kinase, relieves hRKIP's inhibition of the Ras/Raf1/MEK/ERK signaling pathway, and enhances ERK phosphorylation. Cefotetan can be used to study diseases associated with dysregulated Ras/Raf1/MEK/ERK signaling pathways. Cefotetan is also a broad-spectrum antibacterial agent that disrupts cell wall synthesis by binding to bacterial penicillin-binding proteins (PBPs). It is used to study bacterial infections such as bone, skin, urinary tract, and lower respiratory tract.

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Cefotetan

セフォテタン 構造式

CAS 番号 : 69712-56-7

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 63 在庫あり
Solution
10 mM * 1 mL in DMSO USD 63 在庫あり
Solid
5 mg $50 在庫あり
10 mg $75 在庫あり
25 mg $115 在庫あり
50 mg $170 在庫あり
100 mg $225 在庫あり
200 mg $340 在庫あり
500 mg $675 在庫あり
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Based on 0 publication(s) in Google Scholar

Other Forms of Cefotetan:

セフォテタン 関連抗体

Top Publications Citing Use of Products

Antibiotic アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

Raf アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
B-Raf C-Raf Raf

ERK アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
ERK ERK1 ERK2 ERK5 ERK8

Ras アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
K-Ras H-Ras N-Ras Ras

MEK アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
MEK1 MEK2 MEK5 MEK MAP2K4/MEK4 MAP2K7/MEK7

  • 生物活性

  • 純度とドキュメンテーション

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製品説明

Cefotetan is a binding agent that targets human Raf1 kinase inhibitor protein (hRKIP). Cefotetan binds to hRKIP, reduces the binding space between hRKIP and Raf1 kinase, relieves hRKIP's inhibition of the Ras/Raf1/MEK/ERK signaling pathway, and enhances ERK phosphorylation. Cefotetan can be used to study diseases associated with dysregulated Ras/Raf1/MEK/ERK signaling pathways. Cefotetan is also a broad-spectrum antibacterial agent that disrupts cell wall synthesis by binding to bacterial penicillin-binding proteins (PBPs). It is used to study bacterial infections such as bone, skin, urinary tract, and lower respiratory tract[1][2][3].

IC50 & Target

β-lactam

 

Cellular Effect
Cell Line Type Value Description References
HEK293 CC50
>32 μg/mL
Compound: Cefotetan
Cytotoxicity against HEK293 cells incubated for 20 hrs by resazurin dye based assay
Cytotoxicity against HEK293 cells incubated for 20 hrs by resazurin dye based assay
[PMID: 38112278]
体外実験

Cefotetan (20-80 μM; 30 min) significantly increases HEK293T and HeLa cells ERK phosphorylation levels, and this effect is more pronounced in cells overexpressing hRKIP[1].
Cefotetan is active against both Gram-positive bacteria (such as Staphylococci and Streptococci) and Gram-negative bacteria (such as Enterobacter and Haemophilus influenzae), and is highly stable against plasmid- and chromosome-mediated β-lactamases. 0.5 mg/L Cefotetan inhibits 75% of Enterobacter, 4 mg/L Cefotetan inhibits 90% of Enterobacter, and the MIC of Cefotetan against Neisseria gonorrhoeae is 0.06-2 mg/L[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

セフォテタン 関連抗体

Cell Viability Assay[1]

Cell Line: HEK293T cells, HeLa cells, hRKIP-transferred HEK293T cells, hRKIP-transferred HeLa cells
Concentration: 0 μM, 20 μM, 40 μM, 80 μM
Incubation Time: 30 min
Result: Enhanced the phosphorylation level of ERK dose-dependently.
In hRKIP-transferred HEK293T cells, increased the phosphorylation level of ERK by approximately 300% compared with the control at 80 μM; in hRKIP-transferred HeLa cells, increased the phosphorylation level of ERK by approximately 250% under the same concentration.
体内実験

Cefotetan (20, 50, 100 mg/kg; i.v.; single dose) shows that plasma drug concentrations increased with dose in animal models such as mice, rats, dogs and monkeys, with the highest concentration in the kidneys. The urinary excretion rate is 50%-74%, and the biliary excretion rate in rats could reach 48%[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mice (ICR strain, male, 6-7 weeks old), rats (SD strain, male, 6-9 weeks old), rabbits (white rabbits, male, 3.0-3.5 kg), dogs (female beagle dogs, 9.0-14.0 kg), monkeys (female rhesus monkeys, 4.8-5.6 kg)[3]
Dosage: 20 mg/kg
Administration: Intravenous injection, single dose
Result: Mice: 5 minutes after intravenous injection, the plasma concentration reached 52.3 μg/ml, with the highest renal concentration (58.5 μg/g), 24-hour urinary excretion rate of 67.2%, fecal excretion rate of 30.3%; after subcutaneous injection, the fecal excretion rate was 30.3%, and no active metabolites were detected in urine.
Rats: Plasma concentration increased with increasing dose (20 mg/kg group reached 66.5 μg/ml in 5 minutes), with the highest kidney concentration (100 mg/kg group reached 596 μg/g in 5 minutes), 24-hour urine excretion rate of 51.9%, bile excretion rate of 48.1%, and fecal excretion rate of 37.7%.
Rabbit: Plasma concentration of 83 μg/ml in 5 minutes, 74% in 24-hour urine excretion rate, 5.1% in bile excretion rate, and only 0.3% in feces.
Dog: Plasma concentration of 105 μg/ml in 20 mg/kg group in 5 minutes, with the highest kidney concentration (60.3 μg/g in 30 minutes), 52.8% in 24-hour urine excretion rate, 17.4% in bile excretion rate, and 13.3% in feces.
Monkey: 5-minute plasma concentration 129 μg/ml, 24-hour urine excretion rate 59.7%, bile excretion rate not clearly detected, fecal excretion rate 0.3%, plasma protein binding rate 87%.
臨床実験
分子量

575.62

分子式

C17H17N7O8S4
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

O=C(C(N12)=C(CSC3=NN=NN3C)CS[C@]2([H])[C@](OC)(NC(C(S/4)SC4=C(C(O)=O)/C(N)=O)=O)C1=O)O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 175 mg/mL (304.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7373 mL 8.6863 mL 17.3726 mL
5 mM 0.3475 mL 1.7373 mL 3.4745 mL
10 mM 0.1737 mL 0.8686 mL 1.7373 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

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体積 （開始）

V1

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C2

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体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (2.17 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (2.17 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.75%

COA (252 KB) HNMR (212 KB) LCMS (119 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7373 mL 8.6863 mL 17.3726 mL 43.4314 mL
5 mM 0.3475 mL 1.7373 mL 3.4745 mL 8.6863 mL
10 mM 0.1737 mL 0.8686 mL 1.7373 mL 4.3431 mL
15 mM 0.1158 mL 0.5791 mL 1.1582 mL 2.8954 mL
20 mM 0.0869 mL 0.4343 mL 0.8686 mL 2.1716 mL
25 mM 0.0695 mL 0.3475 mL 0.6949 mL 1.7373 mL
30 mM 0.0579 mL 0.2895 mL 0.5791 mL 1.4477 mL
40 mM 0.0434 mL 0.2172 mL 0.4343 mL 1.0858 mL
50 mM 0.0347 mL 0.1737 mL 0.3475 mL 0.8686 mL
60 mM 0.0290 mL 0.1448 mL 0.2895 mL 0.7239 mL
80 mM 0.0217 mL 0.1086 mL 0.2172 mL 0.5429 mL
100 mM 0.0174 mL 0.0869 mL 0.1737 mL 0.4343 mL
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Cefotetan Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cefotetan69712-56-7AntibioticRafERKRasMEKBacterialRaf kinasesExtracellular signal regulated kinasesMitogen-activated protein kinase kinaseMAPKKMAP2KInhibitorinhibitorinhibit

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