Dibucaine
Based on 4 publication(s) in Google Scholar
Dibucaine (Cinchocaine) is a sodium channel inhibitor. Dibucaine is a potent SChE inhibitor.
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- Reinheit: 99.81%
- CAS. Nr.: 85-79-0
- Formel: C20H29N3O2
- Molecular Weight:343.46
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Dibucaine
More
Biologische Aktivität
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Cell Line
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Type | Value | Description | References |
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| A 172 | CC50 |
>30 μM
Compound: 1
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Cytotoxicity against human A172 cells after 3 days by neutral red dye-based photometric method
Cytotoxicity against human A172 cells after 3 days by neutral red dye-based photometric method
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[PMID: 30912944] |
| A549 | CC50 |
37.5 μM
Compound: 1
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Cytotoxicity against human A549 cells after 3 days by neutral red dye-based photometric method
Cytotoxicity against human A549 cells after 3 days by neutral red dye-based photometric method
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[PMID: 30912944] |
| HeLa | CC50 |
36 μM
Compound: 1
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Cytotoxicity against human HeLa cells after 3 days by neutral red dye-based photometric method
Cytotoxicity against human HeLa cells after 3 days by neutral red dye-based photometric method
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[PMID: 30912944] |
| Hepatocyte | IC50 |
470 μM
Compound: 18 Dibucaine
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Inhibition of apamin-sensitive SKCa channel of guinea-pig hepatocytes
Inhibition of apamin-sensitive SKCa channel of guinea-pig hepatocytes
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[PMID: 15225721] |
| RD | CC50 |
>97.049 μM
Compound: 6d
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Cytotoxicity against human RD cells infected with Enterovirus A71 assessed as reduction in cell viability measured by CCK-8 assay
Cytotoxicity against human RD cells infected with Enterovirus A71 assessed as reduction in cell viability measured by CCK-8 assay
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[PMID: 32619885] |
| RD | CC50 |
56.6 μM
Compound: 1
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Cytotoxicity against human RD cells after 3 days by neutral red dye-based photometric method
Cytotoxicity against human RD cells after 3 days by neutral red dye-based photometric method
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[PMID: 30912944] |
| SH-SY5Y | CC50 |
>30 μM
Compound: 1
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Cytotoxicity against human SH-SY5Y cells after 3 days by neutral red dye-based photometric method
Cytotoxicity against human SH-SY5Y cells after 3 days by neutral red dye-based photometric method
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[PMID: 30912944] |
Dibucaine (Cinchocaine) reduces the degradation of BSA-gold complex in the reservosomes, which was not caused either by an inhibition of the whole proteolytic activity of the parasite or by a reduction on the expression levels of cruzipain[1]. Dibucaine, a quaternary ammonium compound, inhibited SChE to a minimum within 2 min in a reversible manner. The inhibition was very potent. It had an IC(50) of 5.3 microM with BuTch or 3.8 microM with AcTch. The inhibition was competitive with respect to BuTch with a K(i) of 1.3 microM and a linear-mixed type (competitive/noncompetitive) with respect to AcTch with inhibition constants, K(i) and K(I) of 0.66 and 2.5 microM, respectively. Dibucaine possesses a butoxy side chain that is similar to the butryl group of BuTch and longer by an ethylene group from AcTch[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 85-79-0
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Appearance Solid
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Molecular Weight 343.46
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Formel C20H29N3O2
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Color White to yellow
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SMILES
O=C(C1=CC(OCCCC)=NC2=CC=CC=C12)NCCN(CC)CC
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Synonyms
Cinchocaine
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (4)
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Journal Impact Factor
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Most Recent
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Biomed Pharmacother
Novel treatment and insight for irradiation-induced injuries: Dibucaine ameliorates irradiation-induced testicular injury by inhibiting fatty acid oxidation in primary Leydig cells. [Abstract]2023 Aug:164:114903. PMID: 37224756 -
Stem Cell Res Ther
Local anesthetics impair the growth and self-renewal of glioblastoma stem cells by inhibiting ZDHHC15-mediated GP130 palmitoylation. [Abstract]2021 Feb 4;12(1):107. PMID: 33541421 -
PLoS Biol
The scale of zebrafish pectoral fin buds is determined by intercellular K+ levels and consequent Ca2+-mediated signaling via retinoic acid regulation of Rcan2 and Kcnk5b. [Abstract]2024 Mar 25;22(3):e3002565. PMID: 38527087 -
Platelets
2022 Apr 3;33(3):381-389. PMID: 33979555
Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (291.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.28 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (270 KB)
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SDS (554 KB)
- English - EN (554 KB)
- Français - FR (554 KB)
- Deutsch - DE (554 KB)
- Norwegian - NO (554 KB)
- Español - ES (554 KB)
- Swedish - SV (554 KB)
- Italian - IT (554 KB)
- Portuguese - PT (554 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Souto-Padron, T., A.P. Lima, and O. Ribeiro Rde, Effects of dibucaine on the endocytic/exocytic pathways in Trypanosoma cruzi. Parasitol Res, 2006. 99(4): p. 317-20. [Content Brief]
[2]. Elamin, B., Dibucaine inhibition of serum cholinesterase. J Biochem Mol Biol, 2003. 36(2): p. 149-53. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9115 mL | 14.5577 mL | 29.1155 mL | 72.7887 mL |
| 5 mM | 0.5823 mL | 2.9115 mL | 5.8231 mL | 14.5577 mL | |
| 10 mM | 0.2912 mL | 1.4558 mL | 2.9115 mL | 7.2789 mL | |
| 15 mM | 0.1941 mL | 0.9705 mL | 1.9410 mL | 4.8526 mL | |
| 20 mM | 0.1456 mL | 0.7279 mL | 1.4558 mL | 3.6394 mL | |
| 25 mM | 0.1165 mL | 0.5823 mL | 1.1646 mL | 2.9115 mL | |
| 30 mM | 0.0971 mL | 0.4853 mL | 0.9705 mL | 2.4263 mL | |
| 40 mM | 0.0728 mL | 0.3639 mL | 0.7279 mL | 1.8197 mL | |
| 50 mM | 0.0582 mL | 0.2912 mL | 0.5823 mL | 1.4558 mL | |
| 60 mM | 0.0485 mL | 0.2426 mL | 0.4853 mL | 1.2131 mL | |
| 80 mM | 0.0364 mL | 0.1820 mL | 0.3639 mL | 0.9099 mL | |
| 100 mM | 0.0291 mL | 0.1456 mL | 0.2912 mL | 0.7279 mL |
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