BNS-22
Based on 1 Customer Validation
BNS-22 is a DNA topoisomerase II (TOP2) catalytic inhibitor with the IC50 values of 2.8 μM and 0.42 μM for human TOP2α and TOP2β, respectively. BNS-22 induces abnormal division and has anti-proliferative activity.
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- Reinheit : 99.50%
- CAS. Nr.: 1151668-24-4
- Formel: C24H25NO5
- Molecular Weight:407.46
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
Beschreibung
IC50 & Target
[1]|
topoisomerase II alpha 2.8 μM (IC50, in human) |
topoisomerase II beta 0.42 μM (IC50, in human) |
In Vitro
BNS-22 (0-30 μM, 24-48 h) can inhibit cell growth and influence on cell cycle progression of the human cervical epidermoid carcinoma cell line HeLa in a dose-dependent manner[1].
BNS-22 (0-30 μM, 0-6 h) does not induce DNA damage and has an antagonistic effect on TOP2 toxin-mediated DNA damage[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HeLa cell lines
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Concentration:0-30 μM
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Incubation Time:24 h, 48 h
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Result:Inhibited cell growth with the IC50 value of 4.9 μM after 24 hours and 1.0 μM after 48 hours.
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Cell Line:HeLa cell lines
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Concentration:0-30 μM
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Incubation Time:24 h
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Result:Increased number of cells in G2/M phase.
Disrupted mitotic spindle formation and induced polyploid cell formation at 3 μM.
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Cell Line:HeLa cell lines
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Concentration:0-30 μM
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Incubation Time:0, 30 min, 1 h, 2 h, 4 h, 6 h
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Result:Decreased etoposide-induced γ-H2AX expression and decreased total cellular TOP2β levels, but not TOP2α levels.
Chemical Information
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CAS. Nr. 1151668-24-4
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Appearance Solid
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Molecular Weight 407.46
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Formel C24H25NO5
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Color White to off-white
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SMILES
O=C1C=C(CCC)C2=C(OC)C=C(OC)C(C(N3CCCC4=C3C=CC=C4)=O)=C2O1
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 50 mg/mL (122.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Protokoll
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Reinheit & Dokumentation
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Data Sheet (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.4542 mL | 12.2711 mL | 24.5423 mL | 61.3557 mL |
| 5 mM | 0.4908 mL | 2.4542 mL | 4.9085 mL | 12.2711 mL | |
| 10 mM | 0.2454 mL | 1.2271 mL | 2.4542 mL | 6.1356 mL | |
| 15 mM | 0.1636 mL | 0.8181 mL | 1.6362 mL | 4.0904 mL | |
| 20 mM | 0.1227 mL | 0.6136 mL | 1.2271 mL | 3.0678 mL | |
| 25 mM | 0.0982 mL | 0.4908 mL | 0.9817 mL | 2.4542 mL | |
| 30 mM | 0.0818 mL | 0.4090 mL | 0.8181 mL | 2.0452 mL | |
| 40 mM | 0.0614 mL | 0.3068 mL | 0.6136 mL | 1.5339 mL | |
| 50 mM | 0.0491 mL | 0.2454 mL | 0.4908 mL | 1.2271 mL | |
| 60 mM | 0.0409 mL | 0.2045 mL | 0.4090 mL | 1.0226 mL | |
| 80 mM | 0.0307 mL | 0.1534 mL | 0.3068 mL | 0.7669 mL | |
| 100 mM | 0.0245 mL | 0.1227 mL | 0.2454 mL | 0.6136 mL |
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