Dictamine
Based on 1 Customer Validation
Dictamnine (Dictamine) exhibits cytotoxicity to human cervical and colon cancer cells and also has antibacterial and antifungal activities.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.16%
- CAS. Nr.: 484-29-7
- Formel: C12H9NO2
- Molecular Weight:199.21
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CAL-27 | IC50 |
19.7 μg/mL
Compound: Dict
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Antiproliferative activity against human CAL-27 cells assessed as cell viability measured after 72 hrs by MTT assay
Antiproliferative activity against human CAL-27 cells assessed as cell viability measured after 72 hrs by MTT assay
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[PMID: 34463097] |
| HepG2 | IC50 |
55.2 μM
Compound: 13
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Growth inhibition of human HepG2 cells after 48 hrs by WST-8 based CCK8 assay
Growth inhibition of human HepG2 cells after 48 hrs by WST-8 based CCK8 assay
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[PMID: 22093759] |
| Neutrophil | IC50 |
>50 μM
Compound: 17
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Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced elastase release
Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced elastase release
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[PMID: 18211005] |
| Neutrophil | IC50 |
>50 μM
Compound: 17
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Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced superoxide anion generation
Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced superoxide anion generation
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[PMID: 18211005] |
| SCC-9 | IC50 |
27.8 μg/mL
Compound: Dict
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Antiproliferative activity against human SCC-9 cells assessed as cell viability measured after 72 hrs by MTT assay
Antiproliferative activity against human SCC-9 cells assessed as cell viability measured after 72 hrs by MTT assay
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[PMID: 34463097] |
Dictamine shows antimicrobial activity against Saccharomyces cerevisiae (MIC: 64 μg/mL)[1].
Dictamine (2 μg/mL, 24 h) reduces ROS and mitochondrial ROS, and reduces IL-1β, TNF-α expression in HaCaT inflammation model[2].
Dictamine (0-100 μM, 12 h) inhibits cell proliferation, migration and invasion, and induces apoptosis in HCT116 cells, by downregulating HIF-1α and Slug. I[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HCT116 cells
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Concentration:0-100 μM
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Incubation Time:12 h
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Result:Inhibited HIF-1α (synthesis but not degradation) and Slug protein accumulation under hypoxia.
Dictamine (50 and 100 mg/kg, p.o., three times a week) inhibits tumor growth in a HCT116 xenograft tumor model[3].
Dictamine (1 and 5 mg/kg, 200 μL, applied to the skin) ameliorates chronic itch in 2,4-dinitrofluorobenzene-induced atopic dermatitis mice[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:HCT116 xenograft tumor model[3]
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Dosage:50 and 100 mg/kg
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Administration:p.o., three times a week
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Result:Inhibited tumor growth.
Reduced HIF-1α and Slug protein level in the tumor tissue.
Chemical Information
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CAS. Nr. 484-29-7
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Appearance Solid
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Molecular Weight 199.21
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Formel C12H9NO2
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Color White to yellow
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SMILES
COC1=C2C(OC=C2)=NC3=CC=CC=C13
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Synonyms
Dictamnine; Dectamine
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Lösungsmittel & Löslichkeit
DMSO : 50 mg/mL (250.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.55 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.55 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Guo N, et al. Global gene expression profile of Saccharomyces cerevisiae induced by dictamnine. Yeast. 2008 Sep;25(9):631-41. [Content Brief]
[2]. An FF, et al. Dihydroartemisinine enhances dictamnine-induced apoptosis via a caspase dependent pathway in human lung adenocarcinoma A549 cells. Asian Pac J Cancer Prev. 2013;14(10):5895-900. [Content Brief]
[3]. Lin CY, et al. Dictamnine delivered by PLGA nanocarriers ameliorated inflammation in an oxazolone-induced dermatitis mouse model. J Control Release. 2021 Jan 10;329:731-742. [Content Brief]
[4]. Wang JY, et al. Dictamnine promotes apoptosis and inhibits epithelial-mesenchymal transition, migration, invasion and proliferation by downregulating the HIF-1α and Slug signaling pathways. Chem Biol Interact. 2018 Dec 25;296:134-144. [Content Brief]
[5]. Yang N, et al. Dictamnine ameliorates chronic itch in DNFB-induced atopic dermatitis mice via inhibiting MrgprA3. Biochem Pharmacol. 2023 Feb;208:115368. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.0198 mL | 25.0991 mL | 50.1983 mL | 125.4957 mL |
| 5 mM | 1.0040 mL | 5.0198 mL | 10.0397 mL | 25.0991 mL | |
| 10 mM | 0.5020 mL | 2.5099 mL | 5.0198 mL | 12.5496 mL | |
| 15 mM | 0.3347 mL | 1.6733 mL | 3.3466 mL | 8.3664 mL | |
| 20 mM | 0.2510 mL | 1.2550 mL | 2.5099 mL | 6.2748 mL | |
| 25 mM | 0.2008 mL | 1.0040 mL | 2.0079 mL | 5.0198 mL | |
| 30 mM | 0.1673 mL | 0.8366 mL | 1.6733 mL | 4.1832 mL | |
| 40 mM | 0.1255 mL | 0.6275 mL | 1.2550 mL | 3.1374 mL | |
| 50 mM | 0.1004 mL | 0.5020 mL | 1.0040 mL | 2.5099 mL | |
| 60 mM | 0.0837 mL | 0.4183 mL | 0.8366 mL | 2.0916 mL | |
| 80 mM | 0.0627 mL | 0.3137 mL | 0.6275 mL | 1.5687 mL | |
| 100 mM | 0.0502 mL | 0.2510 mL | 0.5020 mL | 1.2550 mL |
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