2. Induced Disease Models Products Apoptosis Immunology/Inflammation
  3. Immunology and Inflammatory Disease Models TNF Receptor Interleukin Related
  4. Gastrointestinal Inflammation and Ulcer Models Hapten
  5. Inflammatory Bowel Disease (IBD) Models
  6. Oxazolone

Oxazolone is a haptenizing agent that induces acute or chronic inflammation of the large intestine and is used to construct models of colitis. Oxazolone can cause Th1/Th2-dependent colitis with weight loss and diarrhea. Oxazolone-induced inflammation can be mitigated by neutralizing anti-IL-4 or anti-TNF-α antibodies or decoy IL-13R2-α-FC proteins.

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Oxazolone Chemical Structure

Oxazolone Chemical Structure

CAS No. : 15646-46-5

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Oxazolone Related Antibodies

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Description

Oxazolone is a haptenizing agent that induces acute or chronic inflammation of the large intestine and is used to construct models of colitis. Oxazolone can cause Th1/Th2-dependent colitis with weight loss and diarrhea. Oxazolone-induced inflammation can be mitigated by neutralizing anti-IL-4 or anti-TNF-α antibodies or decoy IL-13R2-α-FC proteins[1].

IC50 & Target[1]

IL-4

 

IL-13

 

TNFR1

 

In Vivo

Colitis induction[1]
1.Prepare procedure
Preparation of sensitization solution (I):
Mix acetone and olive oil in a 4:1 v/v ratio by vortexing. Dissolve a 60 mg oxazolone in 2 mL of this solution to obtain a 3 % (w/v) oxazolone sensitization solution. Mix the solution by carefully vortexing.
Preparation of challenge solution (II):
Dissolve 20 mg Oxazolone in 2 mL of 50 % ethanol to obtain a 1 % (w/v) solution. Mix the solution by careful vortexing. The oxazolone powder should be completely dissolved before use.

2. Experimental procedure
Sensitization pretreatment:
The electric scissors scraped an area of about 2 cmx2 cm on the skin of the mice's backs. Be careful to avoid open wounds.
Apply 150 μL of sensitizing solution (working solution 1) to the exposed skin of mice. Be careful to use an Oxazolone-free sensitizer as a control. Induction treatment:
Anesthetized mice: intraperitoneally injected ketamine/thiazide solution (80 μL/10 g), or inhaled with isoflurane anesthesia system.
The catheter was inserted into the colon of the mice (about 3-4 cm deep at the proximal end of the anus). The other end of the catheter is connected to a 1 mL syringe.
About 100 μL of oxazolone stimulation solution is injected into the colonic cavity through a catheter for 10 to 30 seconds. After dosing, hold the mouse in a vertical position (head down) for 60 seconds.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

217.22

Formula

C12H11NO3
CAS No.
Appearance

Solid

Color

Gray to brown

SMILES

O=C(OC(C1=CC=CC=C1)=N/2)C2=C/OCC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (460.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.6036 mL 23.0181 mL 46.0363 mL
5 mM 0.9207 mL 4.6036 mL 9.2073 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (11.51 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥98.0%

COA (248 KB) HNMR (223 KB) MS (69 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.6036 mL 23.0181 mL 46.0363 mL 115.0907 mL
5 mM 0.9207 mL 4.6036 mL 9.2073 mL 23.0181 mL
10 mM 0.4604 mL 2.3018 mL 4.6036 mL 11.5091 mL
15 mM 0.3069 mL 1.5345 mL 3.0691 mL 7.6727 mL
20 mM 0.2302 mL 1.1509 mL 2.3018 mL 5.7545 mL
25 mM 0.1841 mL 0.9207 mL 1.8415 mL 4.6036 mL
30 mM 0.1535 mL 0.7673 mL 1.5345 mL 3.8364 mL
40 mM 0.1151 mL 0.5755 mL 1.1509 mL 2.8773 mL
50 mM 0.0921 mL 0.4604 mL 0.9207 mL 2.3018 mL
60 mM 0.0767 mL 0.3836 mL 0.7673 mL 1.9182 mL
80 mM 0.0575 mL 0.2877 mL 0.5755 mL 1.4386 mL
100 mM 0.0460 mL 0.2302 mL 0.4604 mL 1.1509 mL
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Oxazolone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Oxazolone15646-46-5TNF ReceptorInterleukin RelatedTumor Necrosis Factor ReceptorTNFRILInhibitorinhibitorinhibit

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