Art. -Nr.: HY-112754A

DOTAP chloride

(Synonyms: 1,2-Dioleoyl-3-trimethylammonium-propane chloride)

19 Cited Publications

Kundenbewertung

Based on 19 publication(s) in Google Scholar

DOTAP chloride is a useful and effective cationic lipid for transient and stable transfection DNA (plasmids, bacmids) and modified nucleic acids (antisense oligonucleotides) with out the use of helper lipid.

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Reinheit: 99.95%
CAS. Nr.: 132172-61-3
Formel: C₄₂H₈₀ClNO₄
Molecular Weight:698.54

Speicherung:

-20°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

DOTAP chloride Chemical Structure

CAS. Nr.: 132172-61-3

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- Clinical Trial
Chemical Information
Publications (19)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC50: Cationic Lipid^[1]

In Vitro

liposomes are reorganized by associating with DNA. The transfection efficiency of DOTAP liposomes is mainly influenced by lipid composition and cell type, but not by size or zeta potential^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 132172-61-3
Appearance <35°C Solid,>38°C Liquid
Molecular Weight 698.54
Formel C₄₂H₈₀ClNO₄
Color White to off-white
SMILES

CCCCCCCC/C=C\CCCCCCCC(OCC(OC(CCCCCCC/C=C\CCCCCCCC)=O)C[N+](C)(C)C)=O.[Cl-]
Synonyms

1,2-Dioleoyl-3-trimethylammonium-propane chloride
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Publications (19)

All Gel Electrophoresis

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Journal Impact Factor

Signal Transduct Target Ther

Intranasal prime-boost RNA vaccination elicits potent T cell response for lung cancer therapy. [Abstract]

2025 Mar 24;10(1):101. PMID: 40122855
Immunity

Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells. [Abstract]

2026 Feb 10;59(2):270-287.e11. PMID: 41672043
Exploration

A pH responsive nanocomposite for combination sonodynamic-immunotherapy with ferroptosis and calcium ion overload via SLC7A11/ACSL4/LPCAT3 pathway. [Abstract]

2024 Jun 26;5(1):20240002. PMID: 40040833
Nat Immunol

Delaying pyroptosis with an AI-screened gasdermin D pore blocker mitigates inflammatory response. [Abstract]

2025 Oct;26(10):1660-1672. PMID: 40954252
J Adv Res

4-Octyl itaconate-induced Nrf2 nuclear translocation mitigates Caspase-11-dependent noncanonical macrophage pyroptosis and acute lung injury. [Abstract]

2026 May 4:S2090-1232(26)00378-4. PMID: 42092588
Cell Rep Med

Engineering lipid nanoparticle-stabilized emulsions for spatiotemporal mRNA delivery and enhanced T cell immunity. [Abstract]

2026 Mar 17;7(3):102667. PMID: 41850240
Carbohydr Polym

Hyaluronic acid-engineered infection-responsive liposomes achieve biofilm penetration and synergistic photothermal-photodynamic antibacterial therapy. [Abstract]

2026 Jul 1:383:125313. PMID: 42067348
Asian J Pharm Sci

2026 Jan 10.
J Control Release

Antimicrobial peptide-derived lipid nanoparticles enable lung-targeted mRNA therapy for multidrug-resistant bacterial pneumonia. [Abstract]

2026 May 23:396:115047. PMID: 42178062
Adv Healthc Mater

Antitumor Activity of Anti-miR-21 Delivered through Lipid Nanoparticles. [Abstract]

2023 Jan;12(6):e2202412. PMID: 36412002
Pharmaceutics

MiR-126-Loaded Immunoliposomes against Vascular Endothelial Inflammation In Vitro and Vivo Evaluation. [Abstract]

2023 Apr 30;15(5):1379. PMID: 37242620

DOTAP chloride purchased from MedChemExpress. Usage Cited in: Pharmaceutics. 2023 Apr 30;15(5):1379. [Abstract]

(A) Identification of miR-126 conjugates using gel retardation assay. 1, miR-126; 2, Va-CLs; 3–8, DOTAP (DOTAP chloride):miR-126 = 16:1, 24:1, 32:1, 40:1, 48:1 and 56:1 incubated for 30 min at room temperature to obtain Va-CLs/miR-126 and CLs/miR-126 complexes. (B) The gray value of each miR-126 band was calculated
Nanoscale

Differential cellular responses to FDA-approved nanomedicines: an exploration of albumin-based nanocarriers and liposomes in protein corona formation. [Abstract]

2023 Nov 16;15(44):17825-17838. PMID: 37850423
PLoS Pathog

LL-37 selectively targets Plasmodium-infected erythrocytes and exhibits antimalarial activity. [Abstract]

2026 Mar 17;22(3):e1014062. PMID: 41843625
Microbiol Spectr

Lipid nanoparticle-delivered mRNA vaccine encoding the MOMP of Chlamydia psittaci elicits protective immune responses in BALB/c mice. [Abstract]

2025 Nov 5;13(12):e0143825. PMID: 41190885
bioRxiv

2026 May 4.
bioRxiv

2026 Apr 27.
bioRxiv

Genus-specific remodeling of carbon and energy metabolism facilitates acetoclastic methanogenesis in Methanosarcina spp. and Methanothrix spp. [Abstract]

2025 Oct 1:2025.10.01.679765. PMID: 41256488
University of Washington

2025.
SSRN

2024 Nov 12.

Lösungsmittel & Löslichkeit

In Vitro:

Ethanol : 130 mg/mL (186.10 mM; Need ultrasonic)

DMSO : 25 mg/mL (35.79 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.4316 mL

7.1578 mL

14.3156 mL

5 mM

0.2863 mL

1.4316 mL

2.8631 mL

10 mM

0.1432 mL

0.7158 mL

1.4316 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (3.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (3.58 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (3.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (266 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Kim BK, et al. DOTAP/DOPE ratio and cell type determine transfection efficiency with DOTAP-liposomes.Biochim Biophys Acta. 2015 Oct;1848(10 Pt A):1996-2001. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / Ethanol	1 mM	1.4316 mL	7.1578 mL	14.3156 mL	35.7889 mL
	5 mM	0.2863 mL	1.4316 mL	2.8631 mL	7.1578 mL
	10 mM	0.1432 mL	0.7158 mL	1.4316 mL	3.5789 mL
	15 mM	0.0954 mL	0.4772 mL	0.9544 mL	2.3859 mL
	20 mM	0.0716 mL	0.3579 mL	0.7158 mL	1.7894 mL
	25 mM	0.0573 mL	0.2863 mL	0.5726 mL	1.4316 mL
	30 mM	0.0477 mL	0.2386 mL	0.4772 mL	1.1930 mL
Ethanol	40 mM	0.0358 mL	0.1789 mL	0.3579 mL	0.8947 mL
	50 mM	0.0286 mL	0.1432 mL	0.2863 mL	0.7158 mL
	60 mM	0.0239 mL	0.1193 mL	0.2386 mL	0.5965 mL
	80 mM	0.0179 mL	0.0895 mL	0.1789 mL	0.4474 mL
	100 mM	0.0143 mL	0.0716 mL	0.1432 mL	0.3579 mL

Keywords

DOTAP chloride

19 Cited Publications

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DOTAP chloride

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Ionizable Lipid Compound Library

Publications Citing Use of MedChemExpress (MCE) DOTAP chloride

Biologische Aktivität

Chemical Information

Publications (19)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

DOTAP chloride

Metabolism/Protease Compound Library

Clinical Compound Library

Drug Repurposing Compound Library

Lipid Compound Library

FDA Approved & Pharmacopeial Drug Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Drug Repurposing Compound Library Plus

Clinical Compound Library Plus

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Liposome Library

Ionizable Lipid Compound Library

Publications Citing Use of MedChemExpress (MCE) DOTAP chloride

Biologische Aktivität

Chemical Information

Publications (19)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

DOTAP chloride Related Classifications

HY-112754A Related Classifications