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Art. -Nr.: HY-108314

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GC-7

8 Cited Publications

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Based on 8 publication(s) in Google Scholar

GC7 Sulfate is a deoxyhypusine synthase (DHPS) inhibitor.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

CAS. Nr.: 150333-69-0
Formel: C₈H₂₀N₄
Molecular Weight:172.27

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (268 KB)

GC-7 Chemical Structure

CAS. Nr.: 150333-69-0

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Clinical Trial
Chemical Information
Publications (8)
Protokoll
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

GC7 Sulfate is a deoxyhypusine synthase (DHPS) inhibitor.

IC₅₀ & Target

DHPS^[1]

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	1.48 μM Compound: GC7	Cytotoxicity against human A-375 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Cytotoxicity against human A-375 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 34473510]
B16	IC₅₀	5.25 μM Compound: GC7	Cytotoxicity against mouse B16 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Cytotoxicity against mouse B16 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 34473510]
HaCaT	CC₅₀	3.96 μM Compound: GC7	Cytotoxicity against human HaCaT cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Cytotoxicity against human HaCaT cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 34473510]
SK-MEL-28	IC₅₀	0.79 μM Compound: GC7	Cytotoxicity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Cytotoxicity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 34473510]

In Vitro

The treatment of MYCN2 (±Dox) and BE(2)-C cells with GC7 for 72 h at various concentrations (0.1 to 100 μM) significantly reduces the number of viable cells in a dose-dependent manner. In MYCN2 cells, 5 μM of GC7 inhibits cell viability by ~40 and ~60 %, respectively, compare to untreated control cells. BE(2)-C cells require 25 μM of GC7 to reduce cell viability by ~50 %. Exposure to 10 and 100 μM GC7 for 72 h clearly decreases the levels of total retinoblastoma (Rb) and phosphorylated Rb as well as of Cdk4 protein, and increases the levels of p21 protein^[1]. Between 0 and 20 μM, GC7 induces little cytotoxicity in HCC cells, while higher concentrations of GC7 (50 to 100 μM) significantly inhibit the viability of all five HCC cell lines tested. Newly synthesized ³H-labeled hypusine of eIF5A1/eIF5A2 is rarely detected after 20 μM GC7 treatment, compare to untreated control. The activity of [³H]-spermidine incorporated into HCC cells is significantly decreased by 20 μM GC7 or higher concentration^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 150333-69-0
Molecular Weight 172.27
Formel C₈H₂₀N₄
SMILES

NC(NCCCCCCCN)=N
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (8)

All Cell Proliferation/Viability AssayWBIn Vivo Efficacy Study

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Journal Impact Factor

Nature

SNOR promotes translation restart after dormancy. [Abstract]

2026 May 13. PMID: 42129552
Immunity

Polyamine metabolism controls B-to-Z DNA transition to orchestrate DNA sensor cGAS activity. [Abstract]

2023 Nov 14;56(11):2508-2522.e6. PMID: 37848037
Nat Commun

Polyamine-mediated ferroptosis amplification acts as a targetable vulnerability in cancer. [Abstract]

2024 Mar 19;15(1):2461. PMID: 38504107

GC-7 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Mar 19;15(1):2461. [Abstract]

Cell viability in A549 or HT1080 cells treated with RSL3 following pre-treatment with 1 μM GC7 for 16 h and polyamines.
Acta Pharm Sin B

Spermidine inactivates proteasome activity and enhances ferroptosis in prostate cancer. [Abstract]

2025 Apr;15(4):2095-2113. PMID: 40486852

GC-7 purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Apr;15(4):2095-2113. [Abstract]

PC3 and DU145 cells were pretreated with GC7 (2 μmol/L) for 6 hours, then cultured for another 24 hours with the specified reagent, and cell viability was then assessed.

GC-7 purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Apr;15(4):2095-2113. [Abstract]

Western blot analysis of Hyp-EIF5A, EIF5A, NRF2, and HMOX1 in PC3 cells. Endogenous polyamines in PC3 cells were depleted by incubation with 1 mmol/L DFMO for 2 days. Polyamine-depleted cells were pretreated with GC7 (2 μmol/L) for 6 hours, followed by treatment with Spd and erastin for 8 hours.

GC-7 purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Apr;15(4):2095-2113. [Abstract]

GC 7 (2 mg/kg, intraperitoneal injection). The volume of the PC3 xenograft tumor was monitored every other day, and the tumor weight was divided into 4 groups.
bioRxiv

2026 Mar 15.
SSRN

2025 Dec 4.
SSRN

2025 Nov 6.
Research Square Preprint

2021 Nov.

GC-7 purchased from MedChemExpress. Usage Cited in: Research Square Preprint. 2021 Nov.

Experimental results show that GC7 inhibits hypoxanthine modification of eIF5A in a concentration-dependent manner.

Protokoll

Cell Assay^[1]

Cell viability is analyzed with the MTS assay kit in accordance with the manufacturer’s protocols. Briefly, after treatment with GC7 for 72 h at various concentrations (0.1 to 100 μM) in 96-well plates, cells are directly incubated with the MTS dye reagent for 1 h at 37°C in a 5 % CO₂ atmosphere. The absorbance is read at 490 nm using a reader^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (268 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Bandino A, et al. Deoxyhypusine synthase (DHPS) inhibitor GC7 induces p21/Rb-mediated inhibition of tumor cell growth and DHPS expression correlates with poor prognosis in neuroblastoma patients. Cell Oncol (Dordr). 2014 Dec;37(6):387-98. [Content Brief]

[2]. Lou B, et al. N1-guanyl-1,7-diaminoheptane (GC7) enhances the therapeutic efficacy of doxorubicin by inhibiting activation of eukaryotic translation initiation factor 5A2 (eIF5A2) and preventing the epithelial-mesenchymal transition in hepatocellular carcinoma cells. Exp Cell Res. 2013 Oct 15;319(17):2708-17. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- Clinical Trial
Chemical Information
Publications (8)
Protokoll
Reinheit & Dokumentation
Verweise

1/16
1/16

GC-7 purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Apr;15(4):2095-2113. [Abstract]

Customer Validation
PC3 and DU145 cells were pretreated with GC7 (2 μmol/L) for 6 hours, then cultured for another 24 hours with the specified reagent, and cell viability was then assessed.
1/16

GC-7 purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Apr;15(4):2095-2113. [Abstract]

Customer Validation
Western blot analysis of Hyp-EIF5A, EIF5A, NRF2, and HMOX1 in PC3 cells. Endogenous polyamines in PC3 cells were depleted by incubation with 1 mmol/L DFMO for 2 days. Polyamine-depleted cells were pretreated with GC7 (2 μmol/L) for 6 hours, followed by treatment with Spd and erastin for 8 hours.
1/16

GC-7 purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Apr;15(4):2095-2113. [Abstract]

Customer Validation
GC 7 (2 mg/kg, intraperitoneal injection). The volume of the PC3 xenograft tumor was monitored every other day, and the tumor weight was divided into 4 groups.
1/16

GC-7 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Mar 19;15(1):2461. [Abstract]

Customer Validation
Cell viability in A549 or HT1080 cells treated with RSL3 following pre-treatment with 1 μM GC7 for 16 h and polyamines.
1/16

GC-7 purchased from MedChemExpress. Usage Cited in: Research Square Preprint. 2021 Nov.

Customer Validation
Experimental results show that GC7 inhibits hypoxanthine modification of eIF5A in a concentration-dependent manner.

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GC-7

Publications Citing Use of MedChemExpress (MCE) GC-7

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