Indole-3-acetamide
Based on 2 publication(s) in Google Scholar
Indole-3-acetamide is a biosynthesis intermediate of 3-Indoleacetic acid (HY-18569). Indole-3-acetic acid is the most common natural plant growth hormone of the auxin class.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.93%
- CAS. Nr.: 879-37-8
- Formel: C10H10N2O
- Molecular Weight:174.20
-
Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biologische Aktivität
-
Chemical Information
-
Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
-
Help & FAQs
-
HY-W007376
Indole-3-carboxaldehydeEndogenous Metabolite -
HY-W028393
Indole-3-pyruvic acidAHR Agonist -
HY-W008253
5-Hydroxyindole-3-acetic acid -
HY-18569
3-Indoleacetic acidPlant Growth Regulator -
HY-W015229
3-Indolepropionic acid -
HY-N0170
Indole-3-carbinolEndogenous Metabolite -
HY-W016784R
Indole-3-acetamide (Standard)Endogenous Metabolite -
HY-113099
Indolelactic acidTryptophan Catabolite -
HY-W007355
SkatoleP38 Activator -
HY-W001132
Indole -
HY-Y0520
Itaconic acidFungi Metabolite -
HY-W010155
TryptopholAromatic Alcohol
Publications Citing Use of MedChemExpress (MCE) Indole-3-acetamide
More
Customer Validation & Images
-
WB
-
ELISA
Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen
More
Biologische Aktivität
Beschreibung
IC50 & Target
|
Human Endogenous Metabolite |
In Vitro
Indole-3-acetamide regulates the production of indole-3-acetic acid, which would be affected by factors such as bacterial growth stage, gene expression, and metal ions. Indole-3-acetic acid is a plant growth hormone that regulates the plants physiological processes, such as cell elongation, differentiation, flowering and fruit development[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
CAS. Nr. 879-37-8
-
Appearance Solid
-
Molecular Weight 174.20
-
Formel C10H10N2O
-
Color White to light brown
-
SMILES
NC(CC1=CNC2=C1C=CC=C2)=O
-
Structure Classification
-
Initial Source
-
Versand
Room temperature in continental US; may vary elsewhere.
-
Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
-
Journal Impact Factor
-
Most Recent
-
Theranostics
Activating AhR alleviates cognitive deficits of Alzheimer's disease model mice by upregulating endogenous Aβ catabolic enzyme Neprilysin. [Abstract]2021 Aug 11;11(18):8797-8812. PMID: 34522212
Indole-3-acetamide purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Aug 11;11(18):8797-8812. [Abstract]
N2a cells were treated with DMSO, Diosmin (Dio; 40 μM), FICZ (250 nM), Indole-3-acetamide (I3C; 10 μM) or L-KN (3 μM) for 12 h. Whole cell extracts were examined by western blotting with the indicated antibodies. NEP expression was adjusted to that of β-actin, which was used as the loading control. n = 3.
Indole-3-acetamide purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Aug 11;11(18):8797-8812. [Abstract]
N2a-APP cells were treated with Diosmin (Dio; 80 μM), FICZ (250 nM), L-KN (3 μM) or Indole-3-acetamide (I3C; 10 μM) with or without SR1 (2 μM) or Sacubitril (Sac; 1 μM) for 24 h, the supernatant was collected and the Aβ42 levels were determined by ELISA.
-
J Environ Sci (China)
Nanoplastics and triclosan co-exposure aggravates DSS-induced colitis in mice by interfering with Akkermansia muciniphila and tryptophan metabolism. [Abstract]2026 Mar:161:189-200. PMID: 41461466
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (574.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 1.3 mg/mL (7.46 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 2.5 mg/mL (14.35 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protokoll
-
Research Protocol for Endocrine Diseases
Endocrine diseases often arise from disrupted hormone production, hormone signaling, or target-tissue responsiveness; for diabetes-focused endocrine disease models, insulin signaling regulates glucose uptake, hepatic glucose output, lipid metabolism, and β-cell compensation. Type 2 diabetes develops through interacting defects in insulin resistance, β-cell dysfunction, adipose inflammation, hepatic glucose overproduction, altered incretin signaling, and ectopic lipid metabolism. A major unresolved question is whether endocrine dysfunction is driven primarily by target-tissue insulin resistance, intrinsic β-cell failure, immune/inflammatory stress, or combined multi-organ failure that differs by disease stage.
Reinheit & Dokumentation
-
Data Sheet (276 KB)
-
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
-
Handling Instructions (2659 KB)
Verweise
[1]. Thomas Lehmann, et al. Indole-3-acetamide-dependent auxin biosynthesis: a widely distributed way of indole-3-acetic acid production? Eur J Cell Biol. 2010 Dec;89(12):895-905. [Content Brief]
[2]. Dimkpa CO, et al., Production of indole-3-acetic acid via the indole-3-acetamide pathway in the plant-beneficial bacterium Pseudomonas chlororaphis O6 is inhibited by ZnO nanoparticles but enhanced by CuO nanoparticles. Appl Environ Microbiol. 2012 Mar;78(5):1404-10. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 5.7405 mL | 28.7026 mL | 57.4053 mL | 143.5132 mL |
| 5 mM | 1.1481 mL | 5.7405 mL | 11.4811 mL | 28.7026 mL | |
| DMSO | 10 mM | 0.5741 mL | 2.8703 mL | 5.7405 mL | 14.3513 mL |
| 15 mM | 0.3827 mL | 1.9135 mL | 3.8270 mL | 9.5675 mL | |
| 20 mM | 0.2870 mL | 1.4351 mL | 2.8703 mL | 7.1757 mL | |
| 25 mM | 0.2296 mL | 1.1481 mL | 2.2962 mL | 5.7405 mL | |
| 30 mM | 0.1914 mL | 0.9568 mL | 1.9135 mL | 4.7838 mL | |
| 40 mM | 0.1435 mL | 0.7176 mL | 1.4351 mL | 3.5878 mL | |
| 50 mM | 0.1148 mL | 0.5741 mL | 1.1481 mL | 2.8703 mL | |
| 60 mM | 0.0957 mL | 0.4784 mL | 0.9568 mL | 2.3919 mL | |
| 80 mM | 0.0718 mL | 0.3588 mL | 0.7176 mL | 1.7939 mL | |
| 100 mM | 0.0574 mL | 0.2870 mL | 0.5741 mL | 1.4351 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Indole-3-acetamide Related Classifications
HY-W016784 Related Classifications
Powered by Bioz