MU876
Based on 1 Customer Validation
MU876 is a MUS81 inhibitor (IC50 0.52 μM). MU876 inhibits Endonuclease activity and disrupts its binding to branched DNA substrates. MU876 inhibits homologous recombination and break-induced replication DNA repair without affecting non-homologous end joining. MU876 induces the accumulation of micronuclei, telomere-free chromosome ends, and DNA damage foci. MU876 sensitizes cancer cells to the cytotoxicity of Cisplatin (HY-17394). MU876 does not induce apoptosis after short-term exposure, nor does it significantly perturb cell cycle distribution at low concentrations.
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- Reinheit : 97.00%
- CAS. Nr.: 1208829-24-6
- Formel: C24H18N6O3
- Molecular Weight:438.44
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Speicherung:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biologische Aktivität
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Biologische Aktivität
Beschreibung
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| U2OS | IC50 |
0.24 μM
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Inhibition of break-induced replication by MU876 in U2OS BIR-GFP reporter cells assessed as reduction in GFP-positive cells by flow cytometry 72 hrs after I-SceI transfection.
Inhibition of break-induced replication by MU876 in U2OS BIR-GFP reporter cells assessed as reduction in GFP-positive cells by flow cytometry 72 hrs after I-SceI transfection.
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41740057 |
| U2OS | IC50 |
0.5-1 μM
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Inhibition of homologous recombination by MU876 in U2OS DR-GFP reporter cells assessed as reduction in GFP-positive cells by flow cytometry 72 hrs after I-SceI transfection.
Inhibition of homologous recombination by MU876 in U2OS DR-GFP reporter cells assessed as reduction in GFP-positive cells by flow cytometry 72 hrs after I-SceI transfection.
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41740057 |
In Vitro
MU876 inhibits BIR in U2OS BIR-GFP cells with an IC50 of 0.24 μM, and inhibits HR with an IC50 of 0.5-1 μM, without impairing NHEJ[1].
MU876 (0.25-0.5 μM; twice weekly for nearly 3 weeks) inhibits the proliferation of CAL51 WT cells over nearly three weeks[1].
MU876 (3-12 μM; 48 h) increases micronucleus formation in U2OS cells[1].
MU876 (72 hours) increases γH2AX foci in U2OS cells after 72 hours[1].
MU876 (maximum concentration 1 μM, 2-fold serial dilution) reduces clonogenic survival of U2OS cells[1].
MU876 enhances Cisplatin (HY-17394)-induced loss of viability in U2OS, HEK293, and CAL51 WT cells, but has no such effect on CAL51 MUS81−/− cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:CAL51 WT cells
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Concentration:0.25 and 0.5 μM
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Incubation Time:twice weekly for almost 3 weeks
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Result:Strongly suppressed proliferation at 0.25 and 0.5 μM.
- CRISPR-Cas9 HDR knock-in/precise editing
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Cell Cytotoxicity Assay
- Mammalian live/dead viability and cytotoxicity staining
- Flow cytometric DNA-content cell-cycle staining
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Apoptosis Solutions
- Protocol for Cell Cycle
- Genotoxicity/Mutagenicity Study
- Cell Viability Determination by MTT Colorimetric Assay
Chemical Information
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CAS. Nr. 1208829-24-6
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Appearance Solid
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Molecular Weight 438.44
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Formel C24H18N6O3
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Color White to off-white
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SMILES
O=C(C1=NOC(C2=CC=CC=C2)=C1)NC3=CC(C4=CC=CC=C4)=NN3C5=NC(C)=CC(N5)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Reinheit & Dokumentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
MU876 Related Classifications
HY-175812 Related Classifications
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