SP-96
Based on 2 publication(s) in Google Scholar
SP-96 is a highly potent, selective and non-ATP-competitive Aurora B (IC50=0.316 nM) inhibitor and shows >2000 fold selectivity against FLT3 and KIT. SP-96 shows selective growth inhibition in NCI60 screening, incluing MDA-MD-468 (GI50=107 nM). SP-96 can be used for the research of triple negative breast cancer (TNBC).
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.65%
- CAS. Nr.: 2682114-54-9
- Formel: C25H20FN7O
- Molecular Weight:453.47
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) SP-96
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Biologische Aktivität
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Aurora A 18.975 nM (IC50) |
Aurora B 0.316 nM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A498 | GI50 |
53.2 nM
Compound: 24; SP-96
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Antiproliferative activity against human A498 cells assessed as cell growth inhibition after 48 hrs by SRB assay
Antiproliferative activity against human A498 cells assessed as cell growth inhibition after 48 hrs by SRB assay
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[PMID: 32717530] |
| CCRF-CEM | GI50 |
47.4 nM
Compound: 24; SP-96
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Antiproliferative activity against human CCRF-CEM cells assessed as cell growth inhibition after 48 hrs by SRB assay
Antiproliferative activity against human CCRF-CEM cells assessed as cell growth inhibition after 48 hrs by SRB assay
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[PMID: 32717530] |
| COLO 205 | GI50 |
50.3 nM
Compound: 24; SP-96
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Antiproliferative activity against human COLO 205 cells assessed as cell growth inhibition after 48 hrs by SRB assay
Antiproliferative activity against human COLO 205 cells assessed as cell growth inhibition after 48 hrs by SRB assay
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[PMID: 32717530] |
| MDA-MB-468 | GI50 |
107 nM
Compound: 24; SP-96
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Antiproliferative activity against human MDA-MB-468 cells assessed as cell growth inhibition after 48 hrs by SRB assay
Antiproliferative activity against human MDA-MB-468 cells assessed as cell growth inhibition after 48 hrs by SRB assay
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[PMID: 32717530] |
SP-96 is a highly potent, selective and non-ATP-competitive Aurora B (IC50=0.316 nM) inhibitor and shows >2000 fold selectivity against FLT3 (IC50=1475.6 nM) and KIT (IC50=1307.6 nM)[1].SP-96 (0-1 μM; 24 hours) is not promiscuous, rather selective for a few cell lines, it inhibits MDA-MB-468, CCRF-CEM, COLO 205 and A498 cell growth with GI50 values of 107 nM,47.4 nM, 50.3 nM and 53.2 nM, respectively[1].SP-96 (63.2 nM) inhibits Aurora B activity in H460 cells by the characteristics of increased DNA content, and it increases cell volume with enormous nucleus[1].SP-96 (0-2 μM) inhibits Aurora B enzymatic activity with an IC50 of 0.316 nM and inhibits Aurora A with observed IC50 value of 18.975 nM. SP-96 shows >2000 fold selectivity against FLT3 (IC50=1475.6 nM) and KIT (IC50=1307.6 nM). Meanwhile, it exhibits inhibitory effects on other receptor tyrosine kinases (RTKs) namely EGFR, RET and HER2 with IC50 value ≥2 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MDA-MB-468, CCRF-CEM, COLO 205 and A498 cell
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Concentration:0-1 µM
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Incubation Time:24 hours
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Result:Showed good inhibitory activity on MDA-MB-468 cells.
Chemical Information
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CAS. Nr. 2682114-54-9
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Appearance Solid
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Molecular Weight 453.47
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Formel C25H20FN7O
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Color White to off-white
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SMILES
O=C(NC1=CC=CC(NC2=C3C=CC(C4=CN(C)N=C4)=CC3=NC=N2)=C1)NC5=CC=CC(F)=C5
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (2)
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Journal Impact Factor
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Most Recent
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Arch Pharm (Weinheim)
Discovery of potent CSK inhibitors through integrated virtual screening and molecular dynamic simulation. [Abstract]2024 Sep;357(9):e2400066. PMID: 38809025 -
Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (220.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2052 mL | 11.0261 mL | 22.0522 mL | 55.1304 mL |
| 5 mM | 0.4410 mL | 2.2052 mL | 4.4104 mL | 11.0261 mL | |
| 10 mM | 0.2205 mL | 1.1026 mL | 2.2052 mL | 5.5130 mL | |
| 15 mM | 0.1470 mL | 0.7351 mL | 1.4701 mL | 3.6754 mL | |
| 20 mM | 0.1103 mL | 0.5513 mL | 1.1026 mL | 2.7565 mL | |
| 25 mM | 0.0882 mL | 0.4410 mL | 0.8821 mL | 2.2052 mL | |
| 30 mM | 0.0735 mL | 0.3675 mL | 0.7351 mL | 1.8377 mL | |
| 40 mM | 0.0551 mL | 0.2757 mL | 0.5513 mL | 1.3783 mL | |
| 50 mM | 0.0441 mL | 0.2205 mL | 0.4410 mL | 1.1026 mL | |
| 60 mM | 0.0368 mL | 0.1838 mL | 0.3675 mL | 0.9188 mL | |
| 80 mM | 0.0276 mL | 0.1378 mL | 0.2757 mL | 0.6891 mL | |
| 100 mM | 0.0221 mL | 0.1103 mL | 0.2205 mL | 0.5513 mL |
SP-96 Related Classifications
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