Cat. No.: HY-N0188

Esculin

5 Cited Publications

Customer Review

Based on 5 publication(s) in Google Scholar

Esculin is a fluorescent coumarin glucoside, the active ingredient in ash bark. Esculin has antidiabetic effects, improves cognitive impairment in experimental diabetic nephropathy (DN) via the MAPK signaling pathway, and exerts antioxidant stress and anti-inflammatory effects. Esculin also has anticancer, antibacterial, antiviral, neuroprotective, antithrombotic, and therapeutic properties for eye diseases.

For research use only. We do not sell to patients.

Purity: 99.85%
CAS No.: 531-75-9
Formula: C₁₅H₁₆O₉
Molecular Weight:340.28

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Esculin Chemical Structure

CAS No.: 531-75-9

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[2]

p38 MAPK

All p38 MAPK Isoforms :

In Vitro

Esculin (100 μM; 2-8 h) has antiviral activity agasint White spot syndrome virus (WSSV) monomer in Litopenaeus vannamei larvae, with a safe concentration of 100 μM^[4].
Esculin (200 μM, 400 μM, 600 μM; 4 h or 2 h) is a chemical protectant of red blood cells, protecting them from oxidative damage. Esculin inhibits the increase of markers of oxidative stress (such as protein and lipid oxidation) induced by Bioallethrin (HY-122376) (50-200 μM; 4 h or 2 h) in red blood cells and lymphocytes, and restores reduced free amino groups. The ratio of reduced glutathione to oxidized glutathione is also increased^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Esculin (5, 10, and 20 mg/kg; Intragastric administration, once daily for 2 days) protects mice model of ethanol-induced gastric injury. Esculin inhibits NF-κB activation, thereby reducing the expression of iNOS, TNF-α and IL-6. Esculin reduces histopathological damage in a dose-dependent manner^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 531-75-9
Appearance Solid
Molecular Weight 340.28
Formula C₁₅H₁₆O₉
Color White to off-white
SMILES

O=C1C=CC2=CC(O[C@H]3[C@@H]([C@H]([C@@H]([C@@H](CO)O3)O)O)O)=C(O)C=C2O1
Structure Classification
- Phenylpropanoids
- Coumarins
- Phenols
- Monophenols
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (5)

All Cell Proliferation/Viability AssayIFFlow CytometryWBIHC

Sort by:

Journal Impact Factor

Phytomedicine

Aesculin suppresses the NLRP3 inflammasome-mediated pyroptosis via the Akt/GSK3β/NF-κB pathway to mitigate myocardial ischemia/reperfusion injury. [Abstract]

2021 Nov:92:153687. PMID: 34482222
Int Immunopharmacol

Esculin suppresses the PERK-eIF2α-CHOP pathway by enhancing SIRT1 expression in oxidative stress-induced rat chondrocytes, mitigating osteoarthritis progression in a rat model. [Abstract]

2024 May 10:132:112061. PMID: 38608474

Esculin purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 May 10:132:112061. [Abstract]

The viability of TBHP-treated (60 μM) chondrocytes after Esculin (0, 50, 100, 200, 300, 400, 500, 600 μM) treatment.

Esculin purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 May 10:132:112061. [Abstract]

Apoptotic chondrocytes were identified through TUNEL fluorescence immunocytochemistry treated with Esculin (300 μM).

Esculin purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 May 10:132:112061. [Abstract]

Flow cytometry was employed to assess chondrocyte apoptosis, with Annexin V and PI fluorescence labeling for detection treated with Esculin (300 μM).

Esculin purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 May 10:132:112061. [Abstract]

The protein levels of Bcl2, Bax, and cleaved caspase3 were measured by western blot treated with Esculin (300 μM).

Esculin purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 May 10:132:112061. [Abstract]

IHC staining was performed to assess the expression of cleaved caspase3, CHOP, and SIRT1 treated with Esculin (200, 400 μg/kg, knee joints injection).
Plant Methods

Regulating bulb dormancy release and flowering in lily through chemical modulation of intercellular communication. [Abstract]

2023 Nov 28;19(1):136. PMID: 38012626
Comput Biol Chem

Integrated strategy of serum metabolomics, network pharmacology and experimental validation on revealing the bioactive metabolites and mechanism of Lycium ruthenicum Murr. against colorectal carcinoma. [Abstract]

2025 Oct 15;120(Pt 2):108721. PMID: 41105991
Plant Direct

Evaluation of pathways to the C-glycosyl isoflavone puerarin in roots of kudzu ( Pueraria montana lobata). [Abstract]

2022 Sep 2;6(9):e442. PMID: 36091880

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (293.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9388 mL

14.6938 mL

29.3876 mL

5 mM

0.5878 mL

2.9388 mL

5.8775 mL

10 mM

0.2939 mL

1.4694 mL

2.9388 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (7.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Knox K, et al. The Coumarin Glucoside, Esculin, Reveals Rapid Changes in Phloem-Transport Velocity in Response to Environmental Cues. Plant Physiol. 2018 Oct;178(2):795-807. [Content Brief]

[2]. Song Y, et al. Esculin ameliorates cognitive impairment in experimental diabetic nephropathy and induces anti-oxidative stress and anti-inflammatory effects via the MAPK pathway. Mol Med Rep. 2018 May;17(5):7395-7402. [Content Brief]

[3]. Li CX, et al. Phytother Res. 2022 Jun;36(6):2434-2448. [Content Brief]

[4]. Li W, et al. Gastroprotective effect of esculin on ethanol-induced gastric lesion in mice. Fundam Clin Pharmacol. 2017 Apr;31(2):174-184. [Content Brief]

[5]. Arif A, et al. Esculin protects human blood cells from bioallethrin-induced toxicity: An ex vivo study. Pestic Biochem Physiol. 2023 Apr;191:105375. [Content Brief]

[6]. Shan LP, et al. Antiviral activity of esculin against white spot syndrome virus: A new starting point for prevention and control of white spot disease outbreaks in shrimp seedling culture. J Fish Dis. 2022 Jan;45(1):59-68. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9388 mL	14.6938 mL	29.3876 mL	73.4689 mL
	5 mM	0.5878 mL	2.9388 mL	5.8775 mL	14.6938 mL
	10 mM	0.2939 mL	1.4694 mL	2.9388 mL	7.3469 mL
	15 mM	0.1959 mL	0.9796 mL	1.9592 mL	4.8979 mL
	20 mM	0.1469 mL	0.7347 mL	1.4694 mL	3.6734 mL
	25 mM	0.1176 mL	0.5878 mL	1.1755 mL	2.9388 mL
	30 mM	0.0980 mL	0.4898 mL	0.9796 mL	2.4490 mL
	40 mM	0.0735 mL	0.3673 mL	0.7347 mL	1.8367 mL
	50 mM	0.0588 mL	0.2939 mL	0.5878 mL	1.4694 mL
	60 mM	0.0490 mL	0.2449 mL	0.4898 mL	1.2245 mL
	80 mM	0.0367 mL	0.1837 mL	0.3673 mL	0.9184 mL
	100 mM	0.0294 mL	0.1469 mL	0.2939 mL	0.7347 mL

Keywords

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Esculin

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

MAPK Compound Library

Natural Product Library

Anti-Cancer Compound Library

Antiviral Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Reprogramming Compound Library

Anti-diabetic Compound Library

Glycoside Compound Library

Oxygen Sensing Compound Library

Antibacterial Compound Library

Medicine Food Homology Compound Library

Phenols Library

Pyroptosis Compound Library

Orally Active Compound Library

Traditional Chinese Medicine Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Neuroprotective Compound Library

Anti-Lung Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Food-Sourced Compound Library

Anti-Cancer Natural Product Library

Antidepressant Compound Library

Antiviral Traditional Chinese Medicine Active Compound Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Pain-Related Compound Library

Serine/Threonine Kinase Inhibitor Library

Anti-Ovarian Cancer Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

Miao Ethnicity Medicine Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Protein Kinase Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Anti-Rheumatic Arthritis Compound Library

Mass Spectrometry Human Metabolite Library

Cosmetic Ingredient Compound Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Posttranslational Modification Library

Mongolian Medicine Compound Library

Chinese Baijiu Compound Library

Natural Product Diversity Scaffold Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Esculin

All p38 MAPK Isoforms

Biological Activity

Chemical Information

Publications (5)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Esculin Related Classifications

HY-N0188 Related Classifications