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H2DCFDA

(Synonyms: DCFH-DA; 2',7'-Dichlorodihydrofluorescein diacetate)
808 Cited Publications
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Based on 808 publication(s) in Google Scholar

H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm).

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  • Pureté: 99.81%
  • CAS No.: 4091-99-0
  • Formule: C24H16Cl2O7
  • Masse moléculaire:487.29
  • Stockage:

    -20°C, protect from light

    * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

  • H2DCFDA Chemical Structure
    H2DCFDA Chemical Structure
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
  • Experimental Validation Results for H2DCFDA
CAS No.: 4091-99-0
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  • H2DCFDA Chemical Structure
    H2DCFDA Chemical Structure
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    H2DCFDA purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Jan 10:e2415030. [Abstract]

    Customer Validation
     DCFH-DA (5 μM; 30 min), a specific indicator for ROS detection, was employed to evaluate overall ROS levels in 4T1 cells.

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Feb 4;19(4):4251-4268. [Abstract]

    Customer Validation
     Inside A375 melanoma cells, the ROS level is measured with the help of the DCFH-DA probe under CLSM imaging.
    Representative CLSM images of ROS generation inside A375 cells after different treatments with or without light irradiation (scale bar: 50 μm; blue: Hoechst 33342, green: DCFH-DA probe; Xe lamp, 650 nm, 6.4 mW/cm2, 12 min).

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Feb 4;19(4):4403-4421. [Abstract]

    Customer Validation
     Flow cytometry analysis of ROS levels in HQMitofactories using the DCFH-DA (10 μM; 30 min) probe. HQ-Mitofactories were exposed to 400 μM H2O2 for 6 h prior to measurement.

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Feb 4;19(4):4403-4421. [Abstract]

    Customer Validation
     DCFH-DA (10 μM; 30 min). Fluorescent images of ROS levels in PC12 cells. ROS were labeled with the DCFH-DA probe (green) and nuclei were stained with Hoechst 33342 (blue). Scale bar, 40 μm. Data are presented as mean ± SD.

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Feb 4;19(4):4403-4421. [Abstract]

    Customer Validation
     Confocal fluorescent images of ROS levels in HQ-Mitofactories. ROS were labeled with the DCFH-DA (10 μM; 30 min) probe (green) and nuclei were stained with Hoechst 33342 (blue). Scale bar, 50 μm.

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Adv Mater. 2024 Nov 26:e2409176. [Abstract]

    Customer Validation
     The ROS levels in the oxygen-glucose deprivation/reoxygenation (OGD/R) model of HT22 cells were detected using DCFH-DA (10 µm; 20 min). Confocal laser scanning microscope (CLSM) images showed bright green fluorescence in the OGD/R group, indicating a significant increase in ROS.

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Adv Mater. 2024 Oct 12:e2411618. [Abstract]

    Customer Validation
     The capability of different biocatalysts to eliminate ROS was assessed through representative images of DCFH-DA (30 min) fluorescence across various cell types (MSCs, RSC96, Raw264.7) subjected to distinct treatments.

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2024 Jul 9:41:30-45. [Abstract]

    Customer Validation
     DCFH-DA (20 min) assay was further examined to evaluate the ROS scavenging capacity on the cellular level. As ROS oxidizes DCFH into DCF, it emits green fluorescence, which can be used to estimate the remaining ROS.

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2024 May 23.

    Customer Validation
     DCFH-DA (10 µM; 20 min). Fluorescence images of DCFH illustrating intracellular ROS in BMSCs after different treatment (scale bar: 200 µm).

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2024 Sep;13(9):e12505. [Abstract]

    Customer Validation
     H2DCFDA (10 µM; 20 min). Detection of intracellular ROS generation in A549 cells exposed to 20 µM H2O2. Scale bar: 200 µm.

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2023 Jan;13(1):113-127. [Abstract]

    Customer Validation
     Treated HL-1 cells were washed three times and incubated with DMEM medium containing H2DCFDA (10 μM; 30 min) at 37 °C. The cells then were washed three times with warm DMEM medium and intracellular ROS production was detected by flow cytometry.

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 May;9(13):e2103317. [Abstract]

    Customer Validation
     CLSM images of HUVEC cells using DCFH‐DA (0.5 hours) as the fluorescent probe: Control, H2O2, H2O2+10 µg/mL1 PDA NPs, H2O2+40 µg/mL PDA NPs, H2O2+80 µg/mL PDA NPs.

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Biomaterials. 2022 Nov:290:121842. [Abstract]

    Customer Validation
     DCFH-DA (10 μM; 30 min) or C11-BODIPY-labeled cells were substantially increased after treatment with 1 μM FeSO4.

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Biomaterials. 2022 May;284:121513. [Abstract]

    Customer Validation
     DCFH-DA (10 μM; 30 min). The ROS generation in 4T1 cells incubated with various formulations for 4 h observed by CLSM.

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Biomaterials. 2022 Jul 21;287:121688. [Abstract]

    Customer Validation
     CLSM images of 4T1 tumor cells stained with DCFH-DA (15 min) after different treatments.

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2022 Oct 5;439:129502. [Abstract]

    Customer Validation
     Representative DCFH-DA (10 μM; 30 min) staining images (Green) in PBNs exposed PSNPs and/or LPS in vivo and in vitro.

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Cell. 2021 Jun 24;184(13):3528-3541.e12. [Abstract]

    Customer Validation
     Stained protoplasts with H2DCFDA for the detection of ROS production and PI for PM integrity and tracked the process by live-cell imaging.

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: ACS Nano. 2021 Oct 26;15(10):16286-16297. [Abstract]

    Customer Validation
     CLSM image of 4T1 cells after being treated with MM, MMP, and MMTP; cells are stained with DCFH-DA (20 μM; for 1 h).

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Biomaterials. 2021 Oct;277:121103. [Abstract]

    Customer Validation
     In vitro experiments are conducted to measure the cellular ROS levels in A549 cells from different treatments via flow cytometry using fluorescent probe H2DCFDA (5 μM; 30 min) to better understand the potential molecular mechanism of SOD@ARA290-HBc on radiation-induced lung injury. H2DCFDA fluorescence intensity in A549 cells significantly increased after irradiation.

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Small. 2021 Aug;17(32):e2101368. [Abstract]

    Customer Validation
     Laser scanning confocal fluorescence microscopy images of HT-1080 cells treated with different nanodrugs for the detection of intracellular lipid peroxidation and total ROS using C11-BODIPY and DCFH-DA (100 nM; 10 min) as the corresponding fluorescence probes.

  • 1/16

    H2DCFDA purchased from MedChemExpress. Usage Cited in: Curr Biol. 2021 Jul 26;31(14):3004-3016.e4. [Abstract]

    Customer Validation
     Co-staining stigmas with H2DCFDA (50 μM; for 1-2 hours) and the cell wall indicator propidium iodide (PI) shows that ROS is located in the cytoplasm of papilla cells, near the periphery of the plasma membrane.

  • H2DCFDA