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Propidium Iodide

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Based on 156 publication(s) in Google Scholar

Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.

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  • Pureté: 99.77%
  • CAS No.: 25535-16-4
  • Formule: C27H34I2N4
  • Masse moléculaire:668.39
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    4°C, sealed storage, away from moisture and light

    * In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)

  • Propidium Iodide Chemical Structure
    Propidium Iodide Chemical Structure
  • Experimental Validation Results for Propidium Iodide
  • Experimental Validation Results for Propidium Iodide
  • Experimental Validation Results for Propidium Iodide
  • Experimental Validation Results for Propidium Iodide
  • Experimental Validation Results for Propidium Iodide
  • Experimental Validation Results for Propidium Iodide
  • Experimental Validation Results for Propidium Iodide
  • Experimental Validation Results for Propidium Iodide
CAS No.: 25535-16-4
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  • Propidium Iodide Chemical Structure
    Propidium Iodide Chemical Structure
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    Propidium Iodide purchased from MedChemExpress. Usage Cited in: ACS Nano. 2023 Feb 28;17(4):3334-3345. [Abstract]

    Customer Validation
     Propidium iodide (PI) staining for cell death with GCFN and ferroptosis inhibitor ferrostatin-1 treatment for 48 h.

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    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2022 Apr 5;920:174830. [Abstract]

    Customer Validation
     RCMs were stained with Propidium Iodide (PI, 2 μg/mL; 2 h) and analyzed using a Nikon Ti2 microscope.

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    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Molecules. 2022 Oct 9;27(19):6733. [Abstract]

    Customer Validation
     A549 cells were treated with erdafitinib (0, 6.25 and 12.5 μM, 24 h) and cell apoptosis was analyzed by flow cytometry and statistically analyzed. The Annexin V+/PI- and Annexin V+/PI+ cells were considered as early and late apoptotic cells, respectively, and the sum of the above two was calculated as apoptotic cells. Propidium iodide (PI, 50 μg/mL) was added for incubation for 30 min in the dark.

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    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Thorac Cancer. 2022 May;13(9):1299-1310. [Abstract]

    Customer Validation
     KYSE450 and ECA109 cells after 96 h various transfections were washed three times in cold PBS, stained with Propidium iodide (PI, 50 μg/mL) and Annexin V‐FITC (25 μg/mL) for 20 min in the dark, and analyzed within 1 h.

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    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Feb;49:102207. [Abstract]

    Customer Validation
     PAMs cells were washed with phosphate-buffered saline (PBS), and then incubated with the nuclear dye propidium iodide for 15 min. The nuclei were counterstained with propidium iodide (red). Fluorescent images were acquired with a confocal laser scanning microscope.

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    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Feb;49:102207. [Abstract]

    Customer Validation
     PK-15CD163 cells were washed with phosphate-buffered saline (PBS), and then incubated with the nuclear dye propidium iodide for 15 min. The nuclei were counterstained with propidium iodide (red). Fluorescent images were acquired with a confocal laser scanning microscope.

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    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2021 Aug 31;2021:5608133. [Abstract]

    Customer Validation
     The NP tissue slices are incubated with a culture medium containing 1 μg/ml Calcein AM and 1 μg/ml PI for one hour at 37°C. After washing with PBS, the slices are immediately viewed using an inverted confocal laser scanning microscope.

  • 1/16

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Aging (Albany NY). 2018 Nov 28;10(11):3353-3370. [Abstract]

    Customer Validation
     KLE cells were stained by 25 μg/mL Propidium Iodide (PI) in fluorescence-activated cell sorting buffer, incubated cells for 30 min in dark environment at room temperature. Cell apoptosis was determined by Annexin V/PI double staining and percentages of cells in each phase (LL, viable; LR, early apoptotic; UL and UR, late apoptotic/necrotic cell) were calculated.

  • Propidium Iodide