Dipivefrin
Dipivefrin is an α/β-adrenoceptor agonist and a prodrug of epinephrine. Dipivefrin regulates intraocular pressure by agonizing α/β-adrenoceptors and modulating the aqueous humor outflow pathway. Dipivefrin acts on neuroblastoma cells and downregulates glutamate-induced intracellular calcium levels. Dipivefrin is used in research related to α/β-adrenoceptor-mediated diseases, such as glaucoma.
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- CAS No.: 52365-63-6
- Formule: C19H29NO5
- Masse moléculaire:351.44
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
IC50 & Target
[1]|
α adrenergic receptor |
β adrenergic receptor |
In Vitro
Dipivefrin (2.8-280 μM; 100 min) significantly inhibits the proliferation of cultured human corneal stromal cells only at a high concentration of 280 μM[3].
Dipivefrin (0.28-28 mM; 100 seconds) dose-dependently inhibits glutamate-induced elevation of [Ca2+]i and decreases basal [Ca2+]i in cultured N1E-115 neuroblastoma cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
Dipivefrin (10 µg/kg; i.p.; before memory retrieval) administered before memory retrieval enhances the ability of alcohol-associated light cues to serve as conditioned reinforcers, indicating bidirectional modulation of alcohol memory reconsolidation through enhanced central adrenergic signaling[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 52365-63-6
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Masse moléculaire 351.44
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Formule C19H29NO5
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SMILES
CC(C)(C(OC1=CC=C(C=C1OC(C(C)(C)C)=O)C(CNC)O)=O)C
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Calcium Spark Assay
Calcium sparks are localized, transient increases in intracellular calcium concentration ([Ca2+]i) that occur in cardiac myocytes and represent elementary events underlying excitation-contraction coupling. These events are generated by the coordinated opening of clusters of ryanodine receptors (RyRs) on the sarcoplasmic reticulum membrane, leading to a brief release of Ca2+ into the cytosol. The detection and analysis of calcium sparks provide insights into the mechanisms of calcium handling and signaling in cardiac cells. Imaging techniques using fluorescent calcium indicators such as Fluo-3 are employed to visualize these subcellular calcium transients with high spatial and temporal resolution. The protocol is based on established methodologies described in primary literature for both experimental measurement and automated analysis of calcium sparks.
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Ca2+ Staining Technique
Ca2+ staining is an experimental technique that utilizes specific fluorescent probes (such as Fluo-4 AM, Fura-2, etc.) to qualitatively or quantitatively detect dynamic changes in intracellular Ca2+ concentrations; this is achieved by monitoring the changes in fluorescent signals generated when these probes bind to free intracellular calcium ions. The underlying principle relies primarily on the presence of chelating groups within the probe's molecular structure that possess high affinity for calcium ions.
Pureté et documentation
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Fiche technique (283 KB)
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SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Dipivefrin Related Classifications
HY-121398 Related Classifications
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