SK48-E26

SK48-E26 effectively captures human recombinant IL-1β in an ELISA format, enabling detection of hrIL-1β at concentrations as low as 0.1 to 0.2 ng/mL with a linear response to IL-1β concentration^[1].
SK48-E26 specifically neutralizes human recombinant IL-1β activity in an EL-4 cell-based bioassay, binds a conformational epitope on hrIL-1β spanning amino acids 95-101, and has an affinity for IL-1β of 0.5 to 3 nM^[1].
Mutations in the heavy chain CDR3 region of SK48-E26 Fab fragments increase affinity for human recombinant IL-1β, with the V120T/R121K mutant showing a 9.79-fold improvement over the wild-type SK48-E26 Fab^[1].
SK48-E26 (10-200 nM) binds to human IL-1β with an equilibrium dissociation constant of 242 pM^[2].
SK48-E26 (1×10^-15-1×10^-7 M; 1 h pre-incubation with IL-1β, 16-20 h incubation with cells) neutralizes human IL-1β with an IC₅₀ of 400 pM and cynomolgus IL-1β with an IC₅₀ of 185 pM in MRC-5 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.