CME-carbodiimide

Name: CME-carbodiimide
Brand: MedChemExpress
SKU: HY-W013741
Rating: 4.5 (1 reviews)

Cat. No.: HY-W013741 Purity: 98.0%: Data Sheet
COA

SDS
Handling Instructions
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CME-carbodiimide is a nucleic acid modification reagent. CME-carbodiimide reacts specifically with uracil and guanine residues of RNA, as well as guanine and thymine residues of denatured DNA; it does not react with native DNA. Modification of DNA by CME-carbodiimide inhibits phosphodiester bond cleavage or DNA hydrolysis mediated by pancreatic ribonuclease, snake venom phosphodiesterase and deoxyribonuclease.

For research use only. We do not sell to patients.

CME-carbodiimide Chemical Structure

CAS No. : 2491-17-0

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
250 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

CME-carbodiimide (10 mg/mL; up to 40 h) binds preferentially to denatured calf-thymus DNA, binding 40 moles per 100 moles of nucleotide in heat-denatured samples, while binding minimally to native calf-thymus DNA and increasing amounts to ultraviolet-irradiated calf-thymus DNA in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

423.57

Formula

C₂₁H₃₃N₃O₄S

CAS No.

2491-17-0

Appearance

Solid

Color

White to off-white

SMILES

C[N+]1(CCN=C=NC2CCCCC2)CCOCC1.O=S(C3=CC=C(C)C=C3)([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature, keep dry and cool

In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (236.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3609 mL	11.8044 mL	23.6088 mL
5 mM	0.4722 mL	2.3609 mL	4.7218 mL
10 mM	0.2361 mL	1.1804 mL	2.3609 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.0%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (253 KB)

Handling Instructions (2659 KB)

References

[1]. Drevitch VF, et al. The reaction of DNA with water-soluble carbodiimide and enzyme sensitivity of modified DNA. Biochim Biophys Acta. 1966 Jul 20;123(1):207-9.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3609 mL	11.8044 mL	23.6088 mL	59.0221 mL
	5 mM	0.4722 mL	2.3609 mL	4.7218 mL	11.8044 mL
	10 mM	0.2361 mL	1.1804 mL	2.3609 mL	5.9022 mL
	15 mM	0.1574 mL	0.7870 mL	1.5739 mL	3.9348 mL
	20 mM	0.1180 mL	0.5902 mL	1.1804 mL	2.9511 mL
	25 mM	0.0944 mL	0.4722 mL	0.9444 mL	2.3609 mL
	30 mM	0.0787 mL	0.3935 mL	0.7870 mL	1.9674 mL
	40 mM	0.0590 mL	0.2951 mL	0.5902 mL	1.4756 mL
	50 mM	0.0472 mL	0.2361 mL	0.4722 mL	1.1804 mL
	60 mM	0.0393 mL	0.1967 mL	0.3935 mL	0.9837 mL
	80 mM	0.0295 mL	0.1476 mL	0.2951 mL	0.7378 mL
	100 mM	0.0236 mL	0.1180 mL	0.2361 mL	0.5902 mL