CP-432
CP-432 (CP-734432) is a EP4 receptor agonist with an IC50 of 2 nM for human sources, 8 nM for canine sources, and an EC50 of 1 nM for human sources. CP-432 stimulates cAMP production and activates β-lactamase (β-lactamase) activity via the cAMP response element signaling pathway. CP-432 is applicable to research related to glaucoma.
For research use only. We do not sell to patients.
- CAS No.: 431990-08-8
- Formula: C23H26F3NO4S
- Molecular Weight:469.52
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All Beta-lactamase Isoforms
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Biological Activity
Description
IC50 & Target
[1]|
human EP4-R 2 nM (IC50) |
dog EP4 8 nM (IC50) |
human EP4-R 1 nM (EC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | EC50 |
0.5 nM
Compound: 16c
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Activity at rat EP4 transfected in HEK293 cells by cAMP accumulation
Activity at rat EP4 transfected in HEK293 cells by cAMP accumulation
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[PMID: 16442794] |
| HEK293 | EC50 |
0.5 nM
Compound: 50, CP432
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Agonist activity at rat EP4 receptor expressed in HEK293 cells assessed as cAMP activation
Agonist activity at rat EP4 receptor expressed in HEK293 cells assessed as cAMP activation
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[PMID: 20218623] |
| HEK293 | IC50 |
483 nM
Compound: 16c
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Binding affinity to rat EP2 receptor expressed in HEK293 cells
Binding affinity to rat EP2 receptor expressed in HEK293 cells
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[PMID: 16442794] |
| HEK293 | IC50 |
6 nM
Compound: 16c
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Binding affinity to rat EP4 receptor expressed in HEK293 cells
Binding affinity to rat EP4 receptor expressed in HEK293 cells
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[PMID: 16442794] |
In Vitro
CP-734432 (20 min) potently stimulates cAMP production in human EP4-expressing HEK293 cells with an EC50 of 1 nM[1].
CP-734432 (3 h pre-incubation; 3 h compound incubation; 2 h substrate incubation) potently activates human EP4 receptor-mediated CRE-β-lactamase signaling in CHO cells with an EC50 of 1 nM, and shows high selectivity over human EP2 receptors[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 431990-08-8
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Molecular Weight 469.52
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Formula C23H26F3NO4S
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SMILES
C(C[C@H](CC1=CC(C(F)(F)F)=CC=C1)O)[C@@H]2N(CCCC=3SC(C(O)=O)=CC3)C(=O)CC2
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Synonyms
CP-734432
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
CP-432 Related Classifications
HY-119236 Related Classifications
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