Baccatin III
Based on 2 publication(s) in Google Scholar
Baccatin III is an orally available, selective inhibitor of the TGF-β1 signaling pathway and myeloid-derived suppressor cell (MDSC) activation. Baccatin III targets the AKT/STAT6 and Smad2/3 pathways, blocking TGF-β1-induced fibroblast differentiation and MDSC-mediated immunosuppression. Baccatin III exerts anti-inflammatory and anti-fibrotic effects by inhibiting macrophage activation and extracellular matrix deposition, and shows potential in the treatment of pulmonary fibrosis and cancer in terms of regulating the tumor immune microenvironment.
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- Reinheit: 99.82%
- CAS. Nr.: 27548-93-2
- Formel: C31H38O11
- Molecular Weight:586.63
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
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Publications Citing Use of MedChemExpress (MCE) Baccatin III
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Biologische Aktivität
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A2780 | IC50 |
>25 μM
Compound: 15
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Antiproliferative activity against human A2780 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
Antiproliferative activity against human A2780 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
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[PMID: 25682561] |
| A549 | IC50 |
>25 μM
Compound: 15
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Antiproliferative activity against human A549 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
Antiproliferative activity against human A549 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
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[PMID: 25682561] |
| A549 | IC50 |
24 μM
Compound: 15
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Antiproliferative activity against human A549/CDDP cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
Antiproliferative activity against human A549/CDDP cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
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[PMID: 25682561] |
| B16 | ED50 |
>1000 nM
Compound: 4
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Cytotoxicity against B16 melanoma cell line.40% inhibition at 1 uM
Cytotoxicity against B16 melanoma cell line.40% inhibition at 1 uM
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[PMID: 1359143] |
| HCT-8 | IC50 |
12 μM
Compound: 15
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Antiproliferative activity against human HCT8 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
Antiproliferative activity against human HCT8 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
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[PMID: 25682561] |
| HepG2 | IC50 |
9.91 μg/mL
Compound: 4
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Cytotoxicity againt human HepG2 cells
Cytotoxicity againt human HepG2 cells
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[PMID: 17239597] |
| KB | IC50 |
10 μg/mL
Compound: 10
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Cytotoxic activity was evaluated against Epidermoid Carcinoma KB cells
Cytotoxic activity was evaluated against Epidermoid Carcinoma KB cells
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[PMID: 9873389] |
| L1210 | IC50 |
>10 μg/mL
Compound: 10
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Cytotoxic activity was evaluated against murine Leukemia L1210 cells
Cytotoxic activity was evaluated against murine Leukemia L1210 cells
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[PMID: 9873389] |
| MCF7 | IC50 |
7.4 μM
Compound: 15
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Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
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[PMID: 25682561] |
| NIH3T3 | IC50 |
>25 μM
Compound: 15
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Antiproliferative activity against mouse NIH/3T3 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
Antiproliferative activity against mouse NIH/3T3 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
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[PMID: 25682561] |
| WI-38 | IC50 |
16.1 μg/mL
Compound: 4
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Cytotoxicity againt human WI38 cells
Cytotoxicity againt human WI38 cells
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[PMID: 17239597] |
| WI-38 VA13 | IC50 |
0.84 μg/mL
Compound: 4
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Cytotoxicity againt human VA13 cells
Cytotoxicity againt human VA13 cells
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[PMID: 17239597] |
Baccatin III (10-100 μM; 16 h) significantly reduces the production of reactive oxygen species (ROS) and nitric oxide (NO) in myeloid-derived suppressor cells (MDSCs) stimulated by LPS/IFN-γ[1].
Baccatin III (3-30 μM; 24 h) dose-dependently inhibits IL-13-induced TGF-β1 mRNA expression in primary mouse lung macrophages[2].
Baccatin III (3-30 μM; 24 h) inhibits TGF-β1-induced mRNA expression of α-SMA, fibronectin and collagen I/III in primary mouse lung fibroblasts[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Baccatin III (5-10 mg/kg; intraperitoneally; single; 21 days) alleviates Bleomycin (HY-108345)-induced lung fibrosis in C57BL/6 mice by inhibiting TGF-β1[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 27548-93-2
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Appearance Solid
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Molecular Weight 586.63
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Formel C31H38O11
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Color White to off-white
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SMILES
O=C1[C@H](OC(C)=O)C(C2(C)C)=C(C)[C@@H](O)C[C@@]2(O)[C@@H](OC(C3=CC=CC=C3)=O)[C@@]4([H])[C@@]1(C)[C@@H](O)C[C@@]5([H])OC[C@]54OC(C)=O
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Nature
2025 Jul;643(8071):582-592. PMID: 40500440 -
bioRxiv
Multiplexed perturbation of yew reveals cryptic proteins that enable a total biosynthesis of baccatin III and Taxol precursors. [Abstract]2024 Nov 16:2024.11.06.622305. PMID: 39574719
Lösungsmittel & Löslichkeit
DMSO : 33.33 mg/mL (56.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.55 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.55 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (285 KB)
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SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Portuguese - PT (644 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Lee YH, et al. Baccatin III, a precursor for the semisynthesis of paclitaxel, inhibits the accumulation and suppressive activity of myeloid-derived suppressor cells in tumor-bearing mice. Int Immunopharmacol. 2014 Aug;21(2):487-93. [Content Brief]
[2]. Nie Y, et al. Baccatin III ameliorates bleomycin-induced pulmonary fibrosis via suppression of TGF-β1 production and TGF-β1-induced fibroblast differentiation. Int Immunopharmacol. 2019 Sep;74:105696. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7047 mL | 8.5233 mL | 17.0465 mL | 42.6163 mL |
| 5 mM | 0.3409 mL | 1.7047 mL | 3.4093 mL | 8.5233 mL | |
| 10 mM | 0.1705 mL | 0.8523 mL | 1.7047 mL | 4.2616 mL | |
| 15 mM | 0.1136 mL | 0.5682 mL | 1.1364 mL | 2.8411 mL | |
| 20 mM | 0.0852 mL | 0.4262 mL | 0.8523 mL | 2.1308 mL | |
| 25 mM | 0.0682 mL | 0.3409 mL | 0.6819 mL | 1.7047 mL | |
| 30 mM | 0.0568 mL | 0.2841 mL | 0.5682 mL | 1.4205 mL | |
| 40 mM | 0.0426 mL | 0.2131 mL | 0.4262 mL | 1.0654 mL | |
| 50 mM | 0.0341 mL | 0.1705 mL | 0.3409 mL | 0.8523 mL |
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