DHX9-IN-5
Based on 1 Customer Validation
DHX9-IN-5 (compound 277) is a RNA helicase DHX9 inhibitor with an IC50 of 4.3 nM.
For research use only. We do not sell to patients.
- Purity: 99.75%
- CAS No.: 2973398-17-1
- Formula: C23H24ClN5O4S
- Molecular Weight:501.99
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
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Helicase |
Chemical Information
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CAS No. 2973398-17-1
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Appearance Solid
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Molecular Weight 501.99
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Formula C23H24ClN5O4S
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Color White to off-white
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SMILES
O=C(C1=CN(C)C(C2=CC=C(N3CC4(C3)COC4)C=N2)=C1)NC5=CC(Cl)=CC(NS(=O)(C)=O)=C5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (199.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (9.96 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.9921 mL | 9.9604 mL | 19.9207 mL | 49.8018 mL |
| 5 mM | 0.3984 mL | 1.9921 mL | 3.9841 mL | 9.9604 mL | |
| 10 mM | 0.1992 mL | 0.9960 mL | 1.9921 mL | 4.9802 mL | |
| 15 mM | 0.1328 mL | 0.6640 mL | 1.3280 mL | 3.3201 mL | |
| 20 mM | 0.0996 mL | 0.4980 mL | 0.9960 mL | 2.4901 mL | |
| 25 mM | 0.0797 mL | 0.3984 mL | 0.7968 mL | 1.9921 mL | |
| 30 mM | 0.0664 mL | 0.3320 mL | 0.6640 mL | 1.6601 mL | |
| 40 mM | 0.0498 mL | 0.2490 mL | 0.4980 mL | 1.2450 mL | |
| 50 mM | 0.0398 mL | 0.1992 mL | 0.3984 mL | 0.9960 mL | |
| 60 mM | 0.0332 mL | 0.1660 mL | 0.3320 mL | 0.8300 mL | |
| 80 mM | 0.0249 mL | 0.1245 mL | 0.2490 mL | 0.6225 mL | |
| 100 mM | 0.0199 mL | 0.0996 mL | 0.1992 mL | 0.4980 mL |
DHX9-IN-5 Related Classifications
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