DOTAP chloride
Based on 19 publication(s) in Google Scholar
DOTAP chloride is a useful and effective cationic lipid for transient and stable transfection DNA (plasmids, bacmids) and modified nucleic acids (antisense oligonucleotides) with out the use of helper lipid.
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 132172-61-3
- Formula: C42H80ClNO4
- Molecular Weight:698.54
-
Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Metabolism/Protease Compound Library
HY-L012
-
Clinical Compound Library
HY-L026
-
Drug Repurposing Compound Library
HY-L035
-
Lipid Compound Library
HY-L043
-
FDA Approved & Pharmacopeial Drug Library
HY-L066
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Drug Repurposing Compound Library Plus
HY-L035P
-
Clinical Compound Library Plus
HY-L026P
-
Bioactive Compound Library
HY-L001
-
High-Throughput Bioactive Compound Library
HY-L205
-
Liposome Library
HY-L214
-
Ionizable Lipid Compound Library
HY-L251
Publications Citing Use of MedChemExpress (MCE) DOTAP chloride
More- Signal Transduct Target Ther. 2025 Mar 24;10(1):101. [Abstract]
- Nat Immunol. 2025 Oct;26(10):1660-1672. [Abstract]
- Immunity. 2026 Feb 10;59(2):270-287.e11. [Abstract]
- Exploration. 2024 Jun 26;5(1):20240002. [Abstract]
- J Adv Res. 2026 May 4:S2090-1232(26)00378-4. [Abstract]
- Carbohydr Polym. 2026 Jul 1:383:125313. [Abstract]
- Asian J Pharm Sci. 2026 Jan 10.
- J Control Release. 2026 May 23:396:115047. [Abstract]
- Cell Rep Med. 2026 Mar 17;7(3):102667. [Abstract]
- Adv Healthc Mater. 2023 Jan;12(6):e2202412. [Abstract]
- Pharmaceutics. 2023 Apr 30;15(5):1379. [Abstract]
- Nanoscale. 2023 Nov 16;15(44):17825-17838. [Abstract]
- PLoS Pathog. 2026 Mar 17;22(3):e1014062. [Abstract]
- Microbiol Spectr. 2025 Nov 5;13(12):e0143825. [Abstract]
- bioRxiv. 2026 May 4.
- bioRxiv. 2026 Apr 27.
- bioRxiv. 2025 Oct 1:2025.10.01.679765. [Abstract]
- University of Washington. 2025.
- SSRN. 2024 Nov 12.
Customer Validation & Images
-
Gel Electrophoresis
Biological Activity
IC50: Cationic Lipid[1]
liposomes are reorganized by associating with DNA. The transfection efficiency of DOTAP liposomes is mainly influenced by lipid composition and cell type, but not by size or zeta potential[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
-
CAS No. 132172-61-3
-
Appearance <35°C Solid,>38°C Liquid
-
Molecular Weight 698.54
-
Formula C42H80ClNO4
-
Color White to off-white
-
SMILES
CCCCCCCC/C=C\CCCCCCCC(OCC(OC(CCCCCCC/C=C\CCCCCCCC)=O)C[N+](C)(C)C)=O.[Cl-]
-
Synonyms
1,2-Dioleoyl-3-trimethylammonium-propane chloride
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (19)
-
Journal Impact Factor
-
Most Recent
-
Signal Transduct Target Ther
Intranasal prime-boost RNA vaccination elicits potent T cell response for lung cancer therapy. [Abstract]2025 Mar 24;10(1):101. PMID: 40122855 -
Nat Immunol
Delaying pyroptosis with an AI-screened gasdermin D pore blocker mitigates inflammatory response. [Abstract]2025 Oct;26(10):1660-1672. PMID: 40954252 -
Immunity
Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells. [Abstract]2026 Feb 10;59(2):270-287.e11. PMID: 41672043 -
Exploration
A pH responsive nanocomposite for combination sonodynamic-immunotherapy with ferroptosis and calcium ion overload via SLC7A11/ACSL4/LPCAT3 pathway. [Abstract]2024 Jun 26;5(1):20240002. PMID: 40040833 -
J Adv Res
4-Octyl itaconate-induced Nrf2 nuclear translocation mitigates Caspase-11-dependent noncanonical macrophage pyroptosis and acute lung injury. [Abstract]2026 May 4:S2090-1232(26)00378-4. PMID: 42092588 -
Carbohydr Polym
Hyaluronic acid-engineered infection-responsive liposomes achieve biofilm penetration and synergistic photothermal-photodynamic antibacterial therapy. [Abstract]2026 Jul 1:383:125313. PMID: 42067348 -
-
J Control Release
Antimicrobial peptide-derived lipid nanoparticles enable lung-targeted mRNA therapy for multidrug-resistant bacterial pneumonia. [Abstract]2026 May 23:396:115047. PMID: 42178062 -
Cell Rep Med
Engineering lipid nanoparticle-stabilized emulsions for spatiotemporal mRNA delivery and enhanced T cell immunity. [Abstract]2026 Mar 17;7(3):102667. PMID: 41850240 -
Adv Healthc Mater
2023 Jan;12(6):e2202412. PMID: 36412002 -
Pharmaceutics
MiR-126-Loaded Immunoliposomes against Vascular Endothelial Inflammation In Vitro and Vivo Evaluation. [Abstract]2023 Apr 30;15(5):1379. PMID: 37242620
DOTAP chloride purchased from MedChemExpress. Usage Cited in: Pharmaceutics. 2023 Apr 30;15(5):1379. [Abstract]
(A) Identification of miR-126 conjugates using gel retardation assay. 1, miR-126; 2, Va-CLs; 3–8, DOTAP (DOTAP chloride):miR-126 = 16:1, 24:1, 32:1, 40:1, 48:1 and 56:1 incubated for 30 min at room temperature to obtain Va-CLs/miR-126 and CLs/miR-126 complexes. (B) The gray value of each miR-126 band was calculated
-
Nanoscale
Differential cellular responses to FDA-approved nanomedicines: an exploration of albumin-based nanocarriers and liposomes in protein corona formation. [Abstract]2023 Nov 16;15(44):17825-17838. PMID: 37850423 -
PLoS Pathog
LL-37 selectively targets Plasmodium-infected erythrocytes and exhibits antimalarial activity. [Abstract]2026 Mar 17;22(3):e1014062. PMID: 41843625 -
Microbiol Spectr
Lipid nanoparticle-delivered mRNA vaccine encoding the MOMP of Chlamydia psittaci elicits protective immune responses in BALB/c mice. [Abstract]2025 Nov 5;13(12):e0143825. PMID: 41190885 -
-
-
bioRxiv
Genus-specific remodeling of carbon and energy metabolism facilitates acetoclastic methanogenesis in Methanosarcina spp. and Methanothrix spp. [Abstract]2025 Oct 1:2025.10.01.679765. PMID: 41256488 -
-
Solvent & Solubility
Ethanol : 130 mg/mL (186.10 mM; Need ultrasonic)
DMSO : 25 mg/mL (35.79 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.58 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (3.58 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (266 KB)
-
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / Ethanol | 1 mM | 1.4316 mL | 7.1578 mL | 14.3156 mL | 35.7889 mL |
| 5 mM | 0.2863 mL | 1.4316 mL | 2.8631 mL | 7.1578 mL | |
| 10 mM | 0.1432 mL | 0.7158 mL | 1.4316 mL | 3.5789 mL | |
| 15 mM | 0.0954 mL | 0.4772 mL | 0.9544 mL | 2.3859 mL | |
| 20 mM | 0.0716 mL | 0.3579 mL | 0.7158 mL | 1.7894 mL | |
| 25 mM | 0.0573 mL | 0.2863 mL | 0.5726 mL | 1.4316 mL | |
| 30 mM | 0.0477 mL | 0.2386 mL | 0.4772 mL | 1.1930 mL | |
| Ethanol | 40 mM | 0.0358 mL | 0.1789 mL | 0.3579 mL | 0.8947 mL |
| 50 mM | 0.0286 mL | 0.1432 mL | 0.2863 mL | 0.7158 mL | |
| 60 mM | 0.0239 mL | 0.1193 mL | 0.2386 mL | 0.5965 mL | |
| 80 mM | 0.0179 mL | 0.0895 mL | 0.1789 mL | 0.4474 mL | |
| 100 mM | 0.0143 mL | 0.0716 mL | 0.1432 mL | 0.3579 mL |
DOTAP chloride Related Classifications
HY-112754A Related Classifications
Powered by Bioz